Abstract
Osteopontin (OPN) is considered as a key protein in cell regeneration. OPN is thought to have many functions in cell-cell binding and cell-matrix binding via OPN receptors in various organs. But there is little information on the precise role of OPN. To clarify the functional role of OPN in tubular injury, we performed in situ hybridization and immunohistochemical analysis of the expression of OPN in a renal cortical necrosis model induced by cisplatin from the acute injury to late recovery phases. In the acute injury phase, both mRNA and protein of OPN were markedly induced in damaged tubular lumens with cell debris. In the late recovery phase, on the other hand, OPN protein and mRNA were observed in dilated and flattened tubular epithelial cells showing a regenerative appearance. Most of these cells were also immunostained with CD44, a receptor of OPN. PCNA staining was also co-localized with these expressions. In light of the CD44 function regulating cell proliferation, these findings suggest that OPN may contribute to regeneration of tubular epithelial cells during the acute to late recovery phases of cortical tubular damage induced by cisplatin.
| Original language | English |
|---|---|
| Journal | Nephron - Experimental Nephrology |
| Volume | 97 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2004 |
| Externally published | Yes |
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Keywords
- Cisplatin
- Osteopontin
- Tubular injury
ASJC Scopus subject areas
- Nephrology
Cite this
Expression of osteopontin in cisplatin-induced tubular injury. / Iguchi, Seitaro; Nishi, Shinichi; Ikegame, Mika; Hoshi, Kazuto; Yoshizawa, Tatsuya; Kawashima, Hiroyuki; Arakawa, Masaaki; Ozawa, Hidehiro; Gejyo, Fumitake.
In: Nephron - Experimental Nephrology, Vol. 97, No. 3, 2004.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of osteopontin in cisplatin-induced tubular injury
AU - Iguchi, Seitaro
AU - Nishi, Shinichi
AU - Ikegame, Mika
AU - Hoshi, Kazuto
AU - Yoshizawa, Tatsuya
AU - Kawashima, Hiroyuki
AU - Arakawa, Masaaki
AU - Ozawa, Hidehiro
AU - Gejyo, Fumitake
PY - 2004
Y1 - 2004
N2 - Osteopontin (OPN) is considered as a key protein in cell regeneration. OPN is thought to have many functions in cell-cell binding and cell-matrix binding via OPN receptors in various organs. But there is little information on the precise role of OPN. To clarify the functional role of OPN in tubular injury, we performed in situ hybridization and immunohistochemical analysis of the expression of OPN in a renal cortical necrosis model induced by cisplatin from the acute injury to late recovery phases. In the acute injury phase, both mRNA and protein of OPN were markedly induced in damaged tubular lumens with cell debris. In the late recovery phase, on the other hand, OPN protein and mRNA were observed in dilated and flattened tubular epithelial cells showing a regenerative appearance. Most of these cells were also immunostained with CD44, a receptor of OPN. PCNA staining was also co-localized with these expressions. In light of the CD44 function regulating cell proliferation, these findings suggest that OPN may contribute to regeneration of tubular epithelial cells during the acute to late recovery phases of cortical tubular damage induced by cisplatin.
AB - Osteopontin (OPN) is considered as a key protein in cell regeneration. OPN is thought to have many functions in cell-cell binding and cell-matrix binding via OPN receptors in various organs. But there is little information on the precise role of OPN. To clarify the functional role of OPN in tubular injury, we performed in situ hybridization and immunohistochemical analysis of the expression of OPN in a renal cortical necrosis model induced by cisplatin from the acute injury to late recovery phases. In the acute injury phase, both mRNA and protein of OPN were markedly induced in damaged tubular lumens with cell debris. In the late recovery phase, on the other hand, OPN protein and mRNA were observed in dilated and flattened tubular epithelial cells showing a regenerative appearance. Most of these cells were also immunostained with CD44, a receptor of OPN. PCNA staining was also co-localized with these expressions. In light of the CD44 function regulating cell proliferation, these findings suggest that OPN may contribute to regeneration of tubular epithelial cells during the acute to late recovery phases of cortical tubular damage induced by cisplatin.
KW - Cisplatin
KW - Osteopontin
KW - Tubular injury
UR - http://www.scopus.com/inward/record.url?scp=4043086986&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4043086986&partnerID=8YFLogxK
U2 - 10.1159/000078643
DO - 10.1159/000078643
M3 - Article
C2 - 15292680
AN - SCOPUS:4043086986
VL - 97
JO - Experimental Nephrology
JF - Experimental Nephrology
SN - 0028-2766
IS - 3
ER -