Expression of Osteonectin in Articular Cartilage of Osteoarthritic Knees

Yoshifumi Nanba, Keiichiro Nishida, Takeshi Yoshikawa, Toru Sato, Hajime Inoue, Yoshinori Kuboki

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The expression of osteonectin (ON) in osteoarthritic articular cartilage was investigated by enzyme immunohistochemistry and colloidal gold immunoelectron microscopy. A total of 96 specimens from 9 knees of 8 patients with osteoarthritis (OA) were examined. In OA cartilage, ON-positive cells varied in distribution and were not seen in all the specimens obtained from the same patient. However, in over half of the specimens (56 of 96), especially in the specimens of Mankin's grades from 4 to 9, which corresponds to relatively early stages of OA, ON was expressed in the cartilage above the calcified layer. On the other hand, ON was detected only in the calcified layer below the tidemark in normal articular cartilage. In addition, colloidal gold immunoelectron microscopy revealed ON in chondrocytes and matrix vesicles (MVs). These findings suggest that ON acts through MVs in the early stages of OA as a significant pathogenetic factor involved in intracartilage calcification, which is known to have a close relationship to the progression of OA.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalActa Medica Okayama
Volume51
Issue number5
Publication statusPublished - Oct 1997

Fingerprint

Osteonectin
Cartilage
Articular Cartilage
Knee
Osteoarthritis
Gold Colloid
Immunoelectron Microscopy
Microscopic examination
Chondrocytes
Immunohistochemistry
Cells
Enzymes

Keywords

  • Calcification
  • Chondrocalcinosis
  • Mankin's histologic-histochemical grading
  • Osteoarthritis
  • Osteonectin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Nanba, Y., Nishida, K., Yoshikawa, T., Sato, T., Inoue, H., & Kuboki, Y. (1997). Expression of Osteonectin in Articular Cartilage of Osteoarthritic Knees. Acta Medica Okayama, 51(5), 239-243.

Expression of Osteonectin in Articular Cartilage of Osteoarthritic Knees. / Nanba, Yoshifumi; Nishida, Keiichiro; Yoshikawa, Takeshi; Sato, Toru; Inoue, Hajime; Kuboki, Yoshinori.

In: Acta Medica Okayama, Vol. 51, No. 5, 10.1997, p. 239-243.

Research output: Contribution to journalArticle

Nanba, Y, Nishida, K, Yoshikawa, T, Sato, T, Inoue, H & Kuboki, Y 1997, 'Expression of Osteonectin in Articular Cartilage of Osteoarthritic Knees', Acta Medica Okayama, vol. 51, no. 5, pp. 239-243.
Nanba Y, Nishida K, Yoshikawa T, Sato T, Inoue H, Kuboki Y. Expression of Osteonectin in Articular Cartilage of Osteoarthritic Knees. Acta Medica Okayama. 1997 Oct;51(5):239-243.
Nanba, Yoshifumi ; Nishida, Keiichiro ; Yoshikawa, Takeshi ; Sato, Toru ; Inoue, Hajime ; Kuboki, Yoshinori. / Expression of Osteonectin in Articular Cartilage of Osteoarthritic Knees. In: Acta Medica Okayama. 1997 ; Vol. 51, No. 5. pp. 239-243.
@article{dacc0251471c467a93161c272605caf9,
title = "Expression of Osteonectin in Articular Cartilage of Osteoarthritic Knees",
abstract = "The expression of osteonectin (ON) in osteoarthritic articular cartilage was investigated by enzyme immunohistochemistry and colloidal gold immunoelectron microscopy. A total of 96 specimens from 9 knees of 8 patients with osteoarthritis (OA) were examined. In OA cartilage, ON-positive cells varied in distribution and were not seen in all the specimens obtained from the same patient. However, in over half of the specimens (56 of 96), especially in the specimens of Mankin's grades from 4 to 9, which corresponds to relatively early stages of OA, ON was expressed in the cartilage above the calcified layer. On the other hand, ON was detected only in the calcified layer below the tidemark in normal articular cartilage. In addition, colloidal gold immunoelectron microscopy revealed ON in chondrocytes and matrix vesicles (MVs). These findings suggest that ON acts through MVs in the early stages of OA as a significant pathogenetic factor involved in intracartilage calcification, which is known to have a close relationship to the progression of OA.",
keywords = "Calcification, Chondrocalcinosis, Mankin's histologic-histochemical grading, Osteoarthritis, Osteonectin",
author = "Yoshifumi Nanba and Keiichiro Nishida and Takeshi Yoshikawa and Toru Sato and Hajime Inoue and Yoshinori Kuboki",
year = "1997",
month = "10",
language = "English",
volume = "51",
pages = "239--243",
journal = "Acta Medica Okayama",
issn = "0386-300X",
publisher = "Okayama University",
number = "5",

}

TY - JOUR

T1 - Expression of Osteonectin in Articular Cartilage of Osteoarthritic Knees

AU - Nanba, Yoshifumi

AU - Nishida, Keiichiro

AU - Yoshikawa, Takeshi

AU - Sato, Toru

AU - Inoue, Hajime

AU - Kuboki, Yoshinori

PY - 1997/10

Y1 - 1997/10

N2 - The expression of osteonectin (ON) in osteoarthritic articular cartilage was investigated by enzyme immunohistochemistry and colloidal gold immunoelectron microscopy. A total of 96 specimens from 9 knees of 8 patients with osteoarthritis (OA) were examined. In OA cartilage, ON-positive cells varied in distribution and were not seen in all the specimens obtained from the same patient. However, in over half of the specimens (56 of 96), especially in the specimens of Mankin's grades from 4 to 9, which corresponds to relatively early stages of OA, ON was expressed in the cartilage above the calcified layer. On the other hand, ON was detected only in the calcified layer below the tidemark in normal articular cartilage. In addition, colloidal gold immunoelectron microscopy revealed ON in chondrocytes and matrix vesicles (MVs). These findings suggest that ON acts through MVs in the early stages of OA as a significant pathogenetic factor involved in intracartilage calcification, which is known to have a close relationship to the progression of OA.

AB - The expression of osteonectin (ON) in osteoarthritic articular cartilage was investigated by enzyme immunohistochemistry and colloidal gold immunoelectron microscopy. A total of 96 specimens from 9 knees of 8 patients with osteoarthritis (OA) were examined. In OA cartilage, ON-positive cells varied in distribution and were not seen in all the specimens obtained from the same patient. However, in over half of the specimens (56 of 96), especially in the specimens of Mankin's grades from 4 to 9, which corresponds to relatively early stages of OA, ON was expressed in the cartilage above the calcified layer. On the other hand, ON was detected only in the calcified layer below the tidemark in normal articular cartilage. In addition, colloidal gold immunoelectron microscopy revealed ON in chondrocytes and matrix vesicles (MVs). These findings suggest that ON acts through MVs in the early stages of OA as a significant pathogenetic factor involved in intracartilage calcification, which is known to have a close relationship to the progression of OA.

KW - Calcification

KW - Chondrocalcinosis

KW - Mankin's histologic-histochemical grading

KW - Osteoarthritis

KW - Osteonectin

UR - http://www.scopus.com/inward/record.url?scp=0031255745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031255745&partnerID=8YFLogxK

M3 - Article

C2 - 9359920

AN - SCOPUS:0031255745

VL - 51

SP - 239

EP - 243

JO - Acta Medica Okayama

JF - Acta Medica Okayama

SN - 0386-300X

IS - 5

ER -