[Expression of OCT4 protein in bladder cancer and its clinicopathological implications].

Pengpeng Zhao, Chunxiao Liu, Kai Xu, Shaobo Zheng, Hulin Li, Yawen Xu, Abai Xu, Bingkun Li, Peng Huang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To study the expression of OCT4 protein in bladder cancer and its correlation to the clinicopathologic features and prognosis of bladder cancer. OCT4 mRNA and protein expression was detected in 5 bladder cancer cell lines (RT-4, Tcc-Sup, KK47, T24, and 5637) and 1 normal bladder cell lines by real-time PCR and Western blotting, respectively. Immunohistochemical analysis was used to detect the expression of OCT4 protein in 46 bladder cancer samples. All the 5 bladder cancer cell lines expressed detectable levels of OCT4 mRNA and proteins, whereas the normal bladder cell line SV-HUC-1 was negative for OCT4 expression. The clinical bladder cancer tissues showed a high positivity rate of OCT4 expression (76.1%), which was not detected in normal bladder tissues. Specific OCT-4 signals were localized mainly in the nuclei of the cancer cells. The expression rate of OCT4 protein was significantly higher in bladder cancer tissue than in normal bladder epithelium (P

Original languageEnglish
Pages (from-to)643-646
Number of pages4
JournalNan fang yi ke da xue xue bao = Journal of Southern Medical University
Volume32
Issue number5
Publication statusPublished - May 2012
Externally publishedYes

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Urinary Bladder Neoplasms
Urinary Bladder
Proteins
Cell Line
Messenger RNA
Cell Nucleus
Real-Time Polymerase Chain Reaction
Epithelium
Western Blotting
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)

Cite this

[Expression of OCT4 protein in bladder cancer and its clinicopathological implications]. / Zhao, Pengpeng; Liu, Chunxiao; Xu, Kai; Zheng, Shaobo; Li, Hulin; Xu, Yawen; Xu, Abai; Li, Bingkun; Huang, Peng.

In: Nan fang yi ke da xue xue bao = Journal of Southern Medical University, Vol. 32, No. 5, 05.2012, p. 643-646.

Research output: Contribution to journalArticle

Zhao, Pengpeng ; Liu, Chunxiao ; Xu, Kai ; Zheng, Shaobo ; Li, Hulin ; Xu, Yawen ; Xu, Abai ; Li, Bingkun ; Huang, Peng. / [Expression of OCT4 protein in bladder cancer and its clinicopathological implications]. In: Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 2012 ; Vol. 32, No. 5. pp. 643-646.
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AU - Li, Bingkun

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