Abstract
To study the expression of OCT4 protein in bladder cancer and its correlation to the clinicopathologic features and prognosis of bladder cancer. OCT4 mRNA and protein expression was detected in 5 bladder cancer cell lines (RT-4, Tcc-Sup, KK47, T24, and 5637) and 1 normal bladder cell lines by real-time PCR and Western blotting, respectively. Immunohistochemical analysis was used to detect the expression of OCT4 protein in 46 bladder cancer samples. All the 5 bladder cancer cell lines expressed detectable levels of OCT4 mRNA and proteins, whereas the normal bladder cell line SV-HUC-1 was negative for OCT4 expression. The clinical bladder cancer tissues showed a high positivity rate of OCT4 expression (76.1%), which was not detected in normal bladder tissues. Specific OCT-4 signals were localized mainly in the nuclei of the cancer cells. The expression rate of OCT4 protein was significantly higher in bladder cancer tissue than in normal bladder epithelium (P
| Original language | English |
|---|---|
| Pages (from-to) | 643-646 |
| Number of pages | 4 |
| Journal | Nan fang yi ke da xue xue bao = Journal of Southern Medical University |
| Volume | 32 |
| Issue number | 5 |
| Publication status | Published - May 2012 |
| Externally published | Yes |
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- Medicine(all)
Cite this
[Expression of OCT4 protein in bladder cancer and its clinicopathological implications]. / Zhao, Pengpeng; Liu, Chunxiao; Xu, Kai; Zheng, Shaobo; Li, Hulin; Xu, Yawen; Xu, Abai; Li, Bingkun; Huang, Peng.
In: Nan fang yi ke da xue xue bao = Journal of Southern Medical University, Vol. 32, No. 5, 05.2012, p. 643-646.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - [Expression of OCT4 protein in bladder cancer and its clinicopathological implications].
AU - Zhao, Pengpeng
AU - Liu, Chunxiao
AU - Xu, Kai
AU - Zheng, Shaobo
AU - Li, Hulin
AU - Xu, Yawen
AU - Xu, Abai
AU - Li, Bingkun
AU - Huang, Peng
PY - 2012/5
Y1 - 2012/5
N2 - To study the expression of OCT4 protein in bladder cancer and its correlation to the clinicopathologic features and prognosis of bladder cancer. OCT4 mRNA and protein expression was detected in 5 bladder cancer cell lines (RT-4, Tcc-Sup, KK47, T24, and 5637) and 1 normal bladder cell lines by real-time PCR and Western blotting, respectively. Immunohistochemical analysis was used to detect the expression of OCT4 protein in 46 bladder cancer samples. All the 5 bladder cancer cell lines expressed detectable levels of OCT4 mRNA and proteins, whereas the normal bladder cell line SV-HUC-1 was negative for OCT4 expression. The clinical bladder cancer tissues showed a high positivity rate of OCT4 expression (76.1%), which was not detected in normal bladder tissues. Specific OCT-4 signals were localized mainly in the nuclei of the cancer cells. The expression rate of OCT4 protein was significantly higher in bladder cancer tissue than in normal bladder epithelium (P
AB - To study the expression of OCT4 protein in bladder cancer and its correlation to the clinicopathologic features and prognosis of bladder cancer. OCT4 mRNA and protein expression was detected in 5 bladder cancer cell lines (RT-4, Tcc-Sup, KK47, T24, and 5637) and 1 normal bladder cell lines by real-time PCR and Western blotting, respectively. Immunohistochemical analysis was used to detect the expression of OCT4 protein in 46 bladder cancer samples. All the 5 bladder cancer cell lines expressed detectable levels of OCT4 mRNA and proteins, whereas the normal bladder cell line SV-HUC-1 was negative for OCT4 expression. The clinical bladder cancer tissues showed a high positivity rate of OCT4 expression (76.1%), which was not detected in normal bladder tissues. Specific OCT-4 signals were localized mainly in the nuclei of the cancer cells. The expression rate of OCT4 protein was significantly higher in bladder cancer tissue than in normal bladder epithelium (P
UR - http://www.scopus.com/inward/record.url?scp=84871919177&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871919177&partnerID=8YFLogxK
M3 - Article
C2 - 22588915
AN - SCOPUS:84871919177
VL - 32
SP - 643
EP - 646
JO - Di yi jun yi da xue xue bao = Academic journal of the First Medical College of PLA
JF - Di yi jun yi da xue xue bao = Academic journal of the First Medical College of PLA
SN - 1673-4254
IS - 5
ER -