Expression of neurotrophins and their receptors tropomyosin-related kinases (Trk) under tension-stress during distraction osteogenesis

Ayako Aiga, Koji Asaumi, You Jin Lee, Hiroaki Kadota, Shigeru Mitani, Toshihumi Ozaki, Masaharu Takigawa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The localization and expression of neurotrophins and their receptors during distraction osteogenesis was investigated in 72 male rat femurs (11 weeks old) to further clarify the concurrence of cellular and molecular events of new bone formation. After osteotomy, a 7-day lag phase was followed by distraction at the rate of 0.25 mm/12 h for 21 days (distraction phase), and a 7-day consolidation phase. The localization of neurotrophins (NGF, BDNF and NT-3) and their receptors tropomyosin-related kinases (TRKA, TRKB and TRKC) by immunostaining showed positive staining in bone forming cells in each stage, although the presence and staining intensity varied by cell type and phase. The expressions of NGF, BDNF and NT-3 by real-time polymerase chain reaction (real-time PCR) showed that the peak of the mRNA expression of NGF occurred 10 days after distraction. NT-3 increased during bone extension, but decreased when distraction stopped. In contrast, BDNF continued to increase gradually throughout the distraction and consolidation phases. These findings suggest that neurotrophins and their receptors may play different roles in endochondral and intramembranous ossification in distraction osteogenesis. The tension stress caused by distraction may stimulate the expression of neurotrophins and their receptors, and promote osteogenesis. Copyright

Original languageEnglish
Pages (from-to)267-277
Number of pages11
JournalActa Medica Okayama
Volume60
Issue number5
Publication statusPublished - Oct 2006

Fingerprint

Nerve Growth Factor Receptors
Distraction Osteogenesis
Tropomyosin
Brain-Derived Neurotrophic Factor
Nerve Growth Factor
Osteogenesis
Bone
Phosphotransferases
Consolidation
Staining and Labeling
Bone and Bones
Polymerase chain reaction
Nerve Growth Factors
Osteotomy
Femur
Rats
Real-Time Polymerase Chain Reaction
Messenger RNA
tropomyosin kinase

Keywords

  • Distraction osteogenesis
  • Mechanical stress
  • Neurotrophin
  • Trk

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Expression of neurotrophins and their receptors tropomyosin-related kinases (Trk) under tension-stress during distraction osteogenesis. / Aiga, Ayako; Asaumi, Koji; Lee, You Jin; Kadota, Hiroaki; Mitani, Shigeru; Ozaki, Toshihumi; Takigawa, Masaharu.

In: Acta Medica Okayama, Vol. 60, No. 5, 10.2006, p. 267-277.

Research output: Contribution to journalArticle

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