Expression of neuropeptide- and hormone-encoding genes in the Ciona intestinalis larval brain

Mayuko Hamada, Naoki Shimozono, Naoyuki Ohta, Yutaka Satou, Takeo Horie, Tsuyoshi Kawada, Honoo Satake, Yasunori Sasakura, Nori Satoh

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Despite containing only approximately 330 cells, the central nervous system (CNS) of Ciona intestinalis larvae has an architecture that is similar to the vertebrate CNS. Although only vertebrates have a distinct hypothalamus-the source of numerous neurohormone peptides that play pivotal roles in the development, function, and maintenance of various neuronal and endocrine systems, it is suggested that the Ciona brain contains a region that corresponds to the vertebrate hypothalamus. To identify genes expressed in the brain, we isolated brain vesicles using transgenic embryos carrying Ci-β-tubulin(promoter)::Kaede, which resulted in robust Kaede expression in the larval CNS. The associated transcriptome was investigated using microarray analysis. We identified 565 genes that were preferentially expressed in the larval brain. Among these genes, 11 encoded neurohormone peptides including such hypothalamic peptides as gonadotropin-releasing hormone and oxytocin/vasopressin. Six of the identified peptide genes had not been previously described. We also found that genes encoding receptors for some of the peptides were expressed in the brain. Interestingly, whole-mount in situ hybridization showed that most of the peptide genes were expressed in the ventral brain. This catalog of the genes expressed in the larval brain should help elucidate the evolution, development, and functioning of the chordate brain.

Original languageEnglish
Pages (from-to)202-214
Number of pages13
JournalDevelopmental Biology
Volume352
Issue number2
DOIs
Publication statusPublished - Apr 15 2011
Externally publishedYes

Keywords

  • Ciona intestinalis
  • Genes expressed in the brain
  • Hormone peptides
  • Microarray
  • Neuropeptides
  • Receptors
  • Transgenic line

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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  • Cite this

    Hamada, M., Shimozono, N., Ohta, N., Satou, Y., Horie, T., Kawada, T., Satake, H., Sasakura, Y., & Satoh, N. (2011). Expression of neuropeptide- and hormone-encoding genes in the Ciona intestinalis larval brain. Developmental Biology, 352(2), 202-214. https://doi.org/10.1016/j.ydbio.2011.01.006