TY - JOUR
T1 - Expression of neurocan after transient middle cerebral artery occlusion in adult rat brain
AU - Deguchi, Kentaro
AU - Takaishi, Mikiro
AU - Hayashi, Takeshi
AU - Oohira, Atsuhiko
AU - Nagotani, Shoko
AU - Li, Feng
AU - Jin, Guang
AU - Nagano, Isao
AU - Shoji, Mikio
AU - Miyazaki, Masahiro
AU - Abe, Koji
AU - Huh, Nam Ho
N1 - Funding Information:
This work was partly supported by a Grant-in-Aid for Scientific Research (B) 15390273 and (Hoga) 15659338 and National Project on Protein Structural and Functional Analyses from the Ministry of Education, Science, Culture and Sports of Japan, and by grants (Itoyama Y, Kimura I and Kuzuhara S) from the Ministry of Health and Welfare of Japan. The authors (M.T. and N.H.) sincerely thank Dr. Taketoshi Ono and Dr. Hisao Nishijo (Toyama Medical and Pharmaceutical University) for their support for this study.
PY - 2005/3/10
Y1 - 2005/3/10
N2 - Neurocan is one of the major chondroitin sulfate proteoglycans in the nervous tissues. The expression and proteolytic cleavage of neurocan are developmentally regulated in the normal rat brain, and the full-length neurocan is detected in juvenile brains but not in normal adult brains. Recently, some studies showed that the full-length neurocan was detectable even in the adult brain when it was exposed to mechanical incision or epileptic stimulation. In the present study, we demonstrated by Western blot analysis that the full-length neurocan transiently appeared in the peri-ischemic region of transient middle cerebral artery occlusion (tMCAO) in adult rat with a peak level at 4 days after tMCAO. Immunohistochemical analysis showed that a clear positive signal of neurocan was observed 4 days after tMCAO in the peri-ischemic region of cerebral cortex and caudate, where cells strongly positive in GFAP expression were also distributed. These results indicate that accumulation of the full-length neurocan produced by reactive astrocytes may be one of the processes for tissue repair and reconstruction of neural networks after focal brain ischemia as well.
AB - Neurocan is one of the major chondroitin sulfate proteoglycans in the nervous tissues. The expression and proteolytic cleavage of neurocan are developmentally regulated in the normal rat brain, and the full-length neurocan is detected in juvenile brains but not in normal adult brains. Recently, some studies showed that the full-length neurocan was detectable even in the adult brain when it was exposed to mechanical incision or epileptic stimulation. In the present study, we demonstrated by Western blot analysis that the full-length neurocan transiently appeared in the peri-ischemic region of transient middle cerebral artery occlusion (tMCAO) in adult rat with a peak level at 4 days after tMCAO. Immunohistochemical analysis showed that a clear positive signal of neurocan was observed 4 days after tMCAO in the peri-ischemic region of cerebral cortex and caudate, where cells strongly positive in GFAP expression were also distributed. These results indicate that accumulation of the full-length neurocan produced by reactive astrocytes may be one of the processes for tissue repair and reconstruction of neural networks after focal brain ischemia as well.
KW - Brain ischemia
KW - Chondroitin sulfate proteoglycan
KW - Neurocan
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U2 - 10.1016/j.brainres.2004.12.016
DO - 10.1016/j.brainres.2004.12.016
M3 - Article
C2 - 15777769
AN - SCOPUS:20144376460
VL - 1037
SP - 194
EP - 199
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1-2
ER -