Abstract
Neural cell adhesion molecules (NCAMs) were immunocytochemically detected in the pituitary glands of female ICR mice and in the pituitary tumor cell lines (GH3 cells and AtT-20 cells) using the antibody against mouse NCAM. Immunoreactive NCAMs (ir-NCAMs) were detected in the cell membranes of mouse pituitary cells, GH3 cells and AtT-20 cells. Fifty four percent of anterior pituitary cells, 58% of neurointermediate cells (cells of both posterior and intermediate lobes), 13% of GH3 cells and 43% of AtT-20 cells expressed ir-NCAMs. Anterior pituitary cell types of NCAM-expressing cells were identified. Most of somatotrophs, gonadotrophs, and thyrotrophs showed NCAM-immunoreactivity, while parts of mammotrophs and corticotrophs did. The cell-type-specific pattern of NCAM expression in the mouse pituitary may be closely associated with physiological roles of pituitary NCAMs. For clarifying roles of pituitary NCAM, AtT-20 cells were treated with antisense oligodeoxynucleotide (ODN) which was complementary to the translation initiation site of NCAM mRNA, or with sense ODN which corresponded to the sense sequence for 7 days. Antisense ODN treatment (100 nM) decreased the percentage of NCAM-expressing cells, and surprisingly increased the cell number of AtT-20 cells compared with sense ODN treatment and non-treatment. This result suggests that NCAM is involved in the growth control of AtT-20 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 269-277 |
| Number of pages | 9 |
| Journal | Biomedical Research |
| Volume | 19 |
| Issue number | 4 |
| Publication status | Published - Aug 1998 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Expression of neural cell adhesion molecules in the mouse anterior pituitary gland and pituitary tumor cells. / Yayoi, Yoshihiro; Takahashi, Sumio; Takeuchi, Sakae; Takeuchi, Makoto; Seki, Tatsunori; Arai, Yasumasa.
In: Biomedical Research, Vol. 19, No. 4, 08.1998, p. 269-277.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of neural cell adhesion molecules in the mouse anterior pituitary gland and pituitary tumor cells
AU - Yayoi, Yoshihiro
AU - Takahashi, Sumio
AU - Takeuchi, Sakae
AU - Takeuchi, Makoto
AU - Seki, Tatsunori
AU - Arai, Yasumasa
PY - 1998/8
Y1 - 1998/8
N2 - Neural cell adhesion molecules (NCAMs) were immunocytochemically detected in the pituitary glands of female ICR mice and in the pituitary tumor cell lines (GH3 cells and AtT-20 cells) using the antibody against mouse NCAM. Immunoreactive NCAMs (ir-NCAMs) were detected in the cell membranes of mouse pituitary cells, GH3 cells and AtT-20 cells. Fifty four percent of anterior pituitary cells, 58% of neurointermediate cells (cells of both posterior and intermediate lobes), 13% of GH3 cells and 43% of AtT-20 cells expressed ir-NCAMs. Anterior pituitary cell types of NCAM-expressing cells were identified. Most of somatotrophs, gonadotrophs, and thyrotrophs showed NCAM-immunoreactivity, while parts of mammotrophs and corticotrophs did. The cell-type-specific pattern of NCAM expression in the mouse pituitary may be closely associated with physiological roles of pituitary NCAMs. For clarifying roles of pituitary NCAM, AtT-20 cells were treated with antisense oligodeoxynucleotide (ODN) which was complementary to the translation initiation site of NCAM mRNA, or with sense ODN which corresponded to the sense sequence for 7 days. Antisense ODN treatment (100 nM) decreased the percentage of NCAM-expressing cells, and surprisingly increased the cell number of AtT-20 cells compared with sense ODN treatment and non-treatment. This result suggests that NCAM is involved in the growth control of AtT-20 cells.
AB - Neural cell adhesion molecules (NCAMs) were immunocytochemically detected in the pituitary glands of female ICR mice and in the pituitary tumor cell lines (GH3 cells and AtT-20 cells) using the antibody against mouse NCAM. Immunoreactive NCAMs (ir-NCAMs) were detected in the cell membranes of mouse pituitary cells, GH3 cells and AtT-20 cells. Fifty four percent of anterior pituitary cells, 58% of neurointermediate cells (cells of both posterior and intermediate lobes), 13% of GH3 cells and 43% of AtT-20 cells expressed ir-NCAMs. Anterior pituitary cell types of NCAM-expressing cells were identified. Most of somatotrophs, gonadotrophs, and thyrotrophs showed NCAM-immunoreactivity, while parts of mammotrophs and corticotrophs did. The cell-type-specific pattern of NCAM expression in the mouse pituitary may be closely associated with physiological roles of pituitary NCAMs. For clarifying roles of pituitary NCAM, AtT-20 cells were treated with antisense oligodeoxynucleotide (ODN) which was complementary to the translation initiation site of NCAM mRNA, or with sense ODN which corresponded to the sense sequence for 7 days. Antisense ODN treatment (100 nM) decreased the percentage of NCAM-expressing cells, and surprisingly increased the cell number of AtT-20 cells compared with sense ODN treatment and non-treatment. This result suggests that NCAM is involved in the growth control of AtT-20 cells.
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M3 - Article
VL - 19
SP - 269
EP - 277
JO - Biomedical Research
JF - Biomedical Research
SN - 0388-6107
IS - 4
ER -