Expression of neural cell adhesion molecules in the mouse anterior pituitary gland and pituitary tumor cells

Yoshihiro Yayoi, Sumio Takahashi, Sakae Takeuchi, Makoto Takeuchi, Tatsunori Seki, Yasumasa Arai

Research output: Contribution to journalArticle

Abstract

Neural cell adhesion molecules (NCAMs) were immunocytochemically detected in the pituitary glands of female ICR mice and in the pituitary tumor cell lines (GH3 cells and AtT-20 cells) using the antibody against mouse NCAM. Immunoreactive NCAMs (ir-NCAMs) were detected in the cell membranes of mouse pituitary cells, GH3 cells and AtT-20 cells. Fifty four percent of anterior pituitary cells, 58% of neurointermediate cells (cells of both posterior and intermediate lobes), 13% of GH3 cells and 43% of AtT-20 cells expressed ir-NCAMs. Anterior pituitary cell types of NCAM-expressing cells were identified. Most of somatotrophs, gonadotrophs, and thyrotrophs showed NCAM-immunoreactivity, while parts of mammotrophs and corticotrophs did. The cell-type-specific pattern of NCAM expression in the mouse pituitary may be closely associated with physiological roles of pituitary NCAMs. For clarifying roles of pituitary NCAM, AtT-20 cells were treated with antisense oligodeoxynucleotide (ODN) which was complementary to the translation initiation site of NCAM mRNA, or with sense ODN which corresponded to the sense sequence for 7 days. Antisense ODN treatment (100 nM) decreased the percentage of NCAM-expressing cells, and surprisingly increased the cell number of AtT-20 cells compared with sense ODN treatment and non-treatment. This result suggests that NCAM is involved in the growth control of AtT-20 cells.

Original languageEnglish
Pages (from-to)269-277
Number of pages9
JournalBiomedical Research
Volume19
Issue number4
Publication statusPublished - Aug 1998

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Neural Cell Adhesion Molecules
Anterior Pituitary Gland
Pituitary Neoplasms
Tumors
Cells
Oligodeoxyribonucleotides
Cell membranes
Thyrotrophs
Corticotrophs
Somatotrophs
Gonadotrophs
Inbred ICR Mouse

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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Expression of neural cell adhesion molecules in the mouse anterior pituitary gland and pituitary tumor cells. / Yayoi, Yoshihiro; Takahashi, Sumio; Takeuchi, Sakae; Takeuchi, Makoto; Seki, Tatsunori; Arai, Yasumasa.

In: Biomedical Research, Vol. 19, No. 4, 08.1998, p. 269-277.

Research output: Contribution to journalArticle

Yayoi, Y, Takahashi, S, Takeuchi, S, Takeuchi, M, Seki, T & Arai, Y 1998, 'Expression of neural cell adhesion molecules in the mouse anterior pituitary gland and pituitary tumor cells', Biomedical Research, vol. 19, no. 4, pp. 269-277.
Yayoi, Yoshihiro ; Takahashi, Sumio ; Takeuchi, Sakae ; Takeuchi, Makoto ; Seki, Tatsunori ; Arai, Yasumasa. / Expression of neural cell adhesion molecules in the mouse anterior pituitary gland and pituitary tumor cells. In: Biomedical Research. 1998 ; Vol. 19, No. 4. pp. 269-277.
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