Neural cell adhesion molecules (NCAMs) were immunocytochemically detected in the pituitary glands of female ICR mice and in the pituitary tumor cell lines (GH3 cells and AtT-20 cells) using the antibody against mouse NCAM. Immunoreactive NCAMs (ir-NCAMs) were detected in the cell membranes of mouse pituitary cells, GH3 cells and AtT-20 cells. Fifty four percent of anterior pituitary cells, 58% of neurointermediate cells (cells of both posterior and intermediate lobes), 13% of GH3 cells and 43% of AtT-20 cells expressed ir-NCAMs. Anterior pituitary cell types of NCAM-expressing cells were identified. Most of somatotrophs, gonadotrophs, and thyrotrophs showed NCAM-immunoreactivity, while parts of mammotrophs and corticotrophs did. The cell-type-specific pattern of NCAM expression in the mouse pituitary may be closely associated with physiological roles of pituitary NCAMs. For clarifying roles of pituitary NCAM, AtT-20 cells were treated with antisense oligodeoxynucleotide (ODN) which was complementary to the translation initiation site of NCAM mRNA, or with sense ODN which corresponded to the sense sequence for 7 days. Antisense ODN treatment (100 nM) decreased the percentage of NCAM-expressing cells, and surprisingly increased the cell number of AtT-20 cells compared with sense ODN treatment and non-treatment. This result suggests that NCAM is involved in the growth control of AtT-20 cells.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)