Expression of netrin-1 and its receptors DCC and neogenin in rat brain after ischemia

Atsushi Tsuchiya, Takeshi Hayashi, Kentaro Deguchi, Yoshihide Sehara, Toru Yamashita, Han Zhe Zhang, Violeta Lukic, Makiko Nagai, Tatsushi Kamiya, Koji Abe

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40 Citations (Scopus)

Abstract

It is very important to investigate the mechanism of axonal growth in the ischemic brain in order to consider a novel mean of therapy for stroke. Netrins are chemotropic factors for axon with chemoattractant or chemorepellant guidance activities, and deleted in colorectal cancer (DCC) and neogenin are receptors for netrins. In this study, we examined expressions of netrin-1, DCC, and neogenin in the brain after 90 min of transient middle cerebral artery occlusion (tMCAO). Netrin-1 was expressed in neurons at the peri-ischemic area with a peak at 14 days. DCC was expressed both in neurons and astrocytic feet with a peak at 14 days, though neogenin was expressed in endothelial cells at MCA territory with a peak at the same time point. These results suggest that netrin-1 is involved in the promotion of axonal growth. The expression of netrin-1 and DCC was overlapped both in the spatial and temporal patterns, indicating that DCC plays a role in netrin-1's axonal growth promoting effects. The location of neogenin positive cells differed from that of netrin-1 positive cells, thus its angiogenic activity may not have relevance with netrin-1.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalBrain Research
Volume1159
Issue number1
DOIs
Publication statusPublished - Jul 23 2007

Keywords

  • Axon growth
  • Brain
  • Ischemia
  • Plasticity
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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    Tsuchiya, A., Hayashi, T., Deguchi, K., Sehara, Y., Yamashita, T., Zhang, H. Z., Lukic, V., Nagai, M., Kamiya, T., & Abe, K. (2007). Expression of netrin-1 and its receptors DCC and neogenin in rat brain after ischemia. Brain Research, 1159(1), 1-7. https://doi.org/10.1016/j.brainres.2006.12.096