Expression of nerve growth factor family neurotrophins in a mouse osteoblastic cell line

Tohru Nakanishi, Kojiro Takahashi, Chiharu Aoki, Kiyoshi Nishikawa, Takako Hattori, Shigehiko Taniguchi

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50 Citations (Scopus)

Abstract

With cultured osteoblastic cells, clone MC3T3-E1 derived from newborn mouse calvaria, the mRNAs encoding three representative neurotrophins, namely, nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), were proved in estimation by the polymerase chain reaction (PCR) method. The increase and then subsequent decrease of all three mRNAs within the proliferation phase were followed by their gradual increase in the differentiation phase, with a tendency of enhancement by exogenous TGF-β as was particularly evident in the case of NGF. These findings were further substantiated by identification of NGF-like neurotrophins in the conditioned medium of the osteoblastic cells by an immunoblotting and neurite extension assay.

Original languageEnglish
Pages (from-to)891-897
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume198
Issue number3
DOIs
Publication statusPublished - Feb 14 1994

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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