Abstract
There is increasing evidence that inflammatory cytokines regulate corpus luteum (CL) function in many species. The purpose of the present study was to determine whether interleukin (IL)-4 and IL-6 are expressed in the porcine CL, and whether these cytokines influence porcine luteal steroidogenesis. The gene expressions of IL-4, IL-6 and their specific receptors were determined in the CL of Chinese Meishan pigs during the estrous cycle. Moreover, the effects of these cytokines on progesterone (P4), estradiol-17β (E2) and prostaglandin (PG) F2α secretion by cultured luteal cells were investigated. IL-4 and IL-6 mRNAs were detected in the CL at all luteal stages. Furthermore, mRNAs of the receptors for IL-4 and IL-6 were clearly expressed in the CL throughout the estrous cycle. Real-time PCR analysis revealed that IL-6 receptor (IL-6R) mRNA expression was higher in the regressed CL (days 19-21 after ovulation) than in the CL at other stages (P <0.01). Exposure of cultured luteal cells obtained from mid-stage CL (days 8-11) to IL-6 (1-100 ng/ml), it inhibited P4 and E2 secretion by the cells (P <0.05). Although IL-4 (1-100 ng/ml) did not significantly alter P4 secretion, it inhibited E2 secretion by the cells (P <0.05). Neither IL-4 nor IL-6 had any effect on PGF2α secretion by the cells. These results suggest that IL-4 and IL-6 are locally produced in the porcine CL, and that they inhibit steroid production from luteal cells via their specific receptors. Collectively, both IL-4 and IL-6 may play roles in regulating porcine CL function throughout the estrous cycle.
| Original language | English |
|---|---|
| Pages (from-to) | 246-257 |
| Number of pages | 12 |
| Journal | Domestic Animal Endocrinology |
| Volume | 31 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Oct 2006 |
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Keywords
- Corpus luteum
- Cytokine
- Pig
- Real-time PCR
- Receptor
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
- Endocrinology
Cite this
Expression of mRNAs for interleukin-4, interleukin-6 and their receptors in porcine corpus luteum during the estrous cycle. / Sakumoto, Ryosuke; Komatsu, Tokushi; Kasuya, Etsuko; Saito, Toshiyuki; Okuda, Kiyoshi.
In: Domestic Animal Endocrinology, Vol. 31, No. 3, 10.2006, p. 246-257.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of mRNAs for interleukin-4, interleukin-6 and their receptors in porcine corpus luteum during the estrous cycle
AU - Sakumoto, Ryosuke
AU - Komatsu, Tokushi
AU - Kasuya, Etsuko
AU - Saito, Toshiyuki
AU - Okuda, Kiyoshi
PY - 2006/10
Y1 - 2006/10
N2 - There is increasing evidence that inflammatory cytokines regulate corpus luteum (CL) function in many species. The purpose of the present study was to determine whether interleukin (IL)-4 and IL-6 are expressed in the porcine CL, and whether these cytokines influence porcine luteal steroidogenesis. The gene expressions of IL-4, IL-6 and their specific receptors were determined in the CL of Chinese Meishan pigs during the estrous cycle. Moreover, the effects of these cytokines on progesterone (P4), estradiol-17β (E2) and prostaglandin (PG) F2α secretion by cultured luteal cells were investigated. IL-4 and IL-6 mRNAs were detected in the CL at all luteal stages. Furthermore, mRNAs of the receptors for IL-4 and IL-6 were clearly expressed in the CL throughout the estrous cycle. Real-time PCR analysis revealed that IL-6 receptor (IL-6R) mRNA expression was higher in the regressed CL (days 19-21 after ovulation) than in the CL at other stages (P <0.01). Exposure of cultured luteal cells obtained from mid-stage CL (days 8-11) to IL-6 (1-100 ng/ml), it inhibited P4 and E2 secretion by the cells (P <0.05). Although IL-4 (1-100 ng/ml) did not significantly alter P4 secretion, it inhibited E2 secretion by the cells (P <0.05). Neither IL-4 nor IL-6 had any effect on PGF2α secretion by the cells. These results suggest that IL-4 and IL-6 are locally produced in the porcine CL, and that they inhibit steroid production from luteal cells via their specific receptors. Collectively, both IL-4 and IL-6 may play roles in regulating porcine CL function throughout the estrous cycle.
AB - There is increasing evidence that inflammatory cytokines regulate corpus luteum (CL) function in many species. The purpose of the present study was to determine whether interleukin (IL)-4 and IL-6 are expressed in the porcine CL, and whether these cytokines influence porcine luteal steroidogenesis. The gene expressions of IL-4, IL-6 and their specific receptors were determined in the CL of Chinese Meishan pigs during the estrous cycle. Moreover, the effects of these cytokines on progesterone (P4), estradiol-17β (E2) and prostaglandin (PG) F2α secretion by cultured luteal cells were investigated. IL-4 and IL-6 mRNAs were detected in the CL at all luteal stages. Furthermore, mRNAs of the receptors for IL-4 and IL-6 were clearly expressed in the CL throughout the estrous cycle. Real-time PCR analysis revealed that IL-6 receptor (IL-6R) mRNA expression was higher in the regressed CL (days 19-21 after ovulation) than in the CL at other stages (P <0.01). Exposure of cultured luteal cells obtained from mid-stage CL (days 8-11) to IL-6 (1-100 ng/ml), it inhibited P4 and E2 secretion by the cells (P <0.05). Although IL-4 (1-100 ng/ml) did not significantly alter P4 secretion, it inhibited E2 secretion by the cells (P <0.05). Neither IL-4 nor IL-6 had any effect on PGF2α secretion by the cells. These results suggest that IL-4 and IL-6 are locally produced in the porcine CL, and that they inhibit steroid production from luteal cells via their specific receptors. Collectively, both IL-4 and IL-6 may play roles in regulating porcine CL function throughout the estrous cycle.
KW - Corpus luteum
KW - Cytokine
KW - Pig
KW - Real-time PCR
KW - Receptor
UR - http://www.scopus.com/inward/record.url?scp=33748195532&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748195532&partnerID=8YFLogxK
U2 - 10.1016/j.domaniend.2005.11.001
DO - 10.1016/j.domaniend.2005.11.001
M3 - Article
C2 - 16332426
AN - SCOPUS:33748195532
VL - 31
SP - 246
EP - 257
JO - Domestic Animal Endocrinology
JF - Domestic Animal Endocrinology
SN - 0739-7240
IS - 3
ER -