TY - JOUR
T1 - Expression of mRNAs for interleukin-4, interleukin-6 and their receptors in porcine corpus luteum during the estrous cycle
AU - Sakumoto, Ryosuke
AU - Komatsu, Tokushi
AU - Kasuya, Etsuko
AU - Saito, Toshiyuki
AU - Okuda, Kiyoshi
N1 - Funding Information:
This research was supported by Grants-in-Aid for Scientific Research (No. 17780212, No. 14360168) from the Japan Society for the Promotion of Science (JSPS). The authors thank all the members of the Swine Management Section of the National Institute of Livestock and Grassland Science for their skilled technical assistance, and Dr. Seiji Ito, Kansai Medical University, for the antiserum against PGF2α.
PY - 2006/10
Y1 - 2006/10
N2 - There is increasing evidence that inflammatory cytokines regulate corpus luteum (CL) function in many species. The purpose of the present study was to determine whether interleukin (IL)-4 and IL-6 are expressed in the porcine CL, and whether these cytokines influence porcine luteal steroidogenesis. The gene expressions of IL-4, IL-6 and their specific receptors were determined in the CL of Chinese Meishan pigs during the estrous cycle. Moreover, the effects of these cytokines on progesterone (P4), estradiol-17β (E2) and prostaglandin (PG) F2α secretion by cultured luteal cells were investigated. IL-4 and IL-6 mRNAs were detected in the CL at all luteal stages. Furthermore, mRNAs of the receptors for IL-4 and IL-6 were clearly expressed in the CL throughout the estrous cycle. Real-time PCR analysis revealed that IL-6 receptor (IL-6R) mRNA expression was higher in the regressed CL (days 19-21 after ovulation) than in the CL at other stages (P < 0.01). Exposure of cultured luteal cells obtained from mid-stage CL (days 8-11) to IL-6 (1-100 ng/ml), it inhibited P4 and E2 secretion by the cells (P < 0.05). Although IL-4 (1-100 ng/ml) did not significantly alter P4 secretion, it inhibited E2 secretion by the cells (P < 0.05). Neither IL-4 nor IL-6 had any effect on PGF2α secretion by the cells. These results suggest that IL-4 and IL-6 are locally produced in the porcine CL, and that they inhibit steroid production from luteal cells via their specific receptors. Collectively, both IL-4 and IL-6 may play roles in regulating porcine CL function throughout the estrous cycle.
AB - There is increasing evidence that inflammatory cytokines regulate corpus luteum (CL) function in many species. The purpose of the present study was to determine whether interleukin (IL)-4 and IL-6 are expressed in the porcine CL, and whether these cytokines influence porcine luteal steroidogenesis. The gene expressions of IL-4, IL-6 and their specific receptors were determined in the CL of Chinese Meishan pigs during the estrous cycle. Moreover, the effects of these cytokines on progesterone (P4), estradiol-17β (E2) and prostaglandin (PG) F2α secretion by cultured luteal cells were investigated. IL-4 and IL-6 mRNAs were detected in the CL at all luteal stages. Furthermore, mRNAs of the receptors for IL-4 and IL-6 were clearly expressed in the CL throughout the estrous cycle. Real-time PCR analysis revealed that IL-6 receptor (IL-6R) mRNA expression was higher in the regressed CL (days 19-21 after ovulation) than in the CL at other stages (P < 0.01). Exposure of cultured luteal cells obtained from mid-stage CL (days 8-11) to IL-6 (1-100 ng/ml), it inhibited P4 and E2 secretion by the cells (P < 0.05). Although IL-4 (1-100 ng/ml) did not significantly alter P4 secretion, it inhibited E2 secretion by the cells (P < 0.05). Neither IL-4 nor IL-6 had any effect on PGF2α secretion by the cells. These results suggest that IL-4 and IL-6 are locally produced in the porcine CL, and that they inhibit steroid production from luteal cells via their specific receptors. Collectively, both IL-4 and IL-6 may play roles in regulating porcine CL function throughout the estrous cycle.
KW - Corpus luteum
KW - Cytokine
KW - Pig
KW - Real-time PCR
KW - Receptor
UR - http://www.scopus.com/inward/record.url?scp=33748195532&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748195532&partnerID=8YFLogxK
U2 - 10.1016/j.domaniend.2005.11.001
DO - 10.1016/j.domaniend.2005.11.001
M3 - Article
C2 - 16332426
AN - SCOPUS:33748195532
VL - 31
SP - 246
EP - 257
JO - Domestic Animal Endocrinology
JF - Domestic Animal Endocrinology
SN - 0739-7240
IS - 3
ER -