Expression of mRNA encoding neurotrophic factors and its regulation in a hybrid neuronal cell line

Tohru Nakanishi, Kazuhiro Ishii, Nobuyuki Fukushima, Masato Asanuma, Emi Iwata, Norio Ogawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In a cultured hybrid neuronal cell line (BIM) which was produced between human neuroblastoma cells (IMR32) and thymidine auxotrophs (B3T) of rat nerve-like cells (B103), the mRNAs encoding ciliary neurotrophic factor (CNTF) and neurotrophins were detected by the polymerase chain reaction method. The conditioned medium of BIM cells enhanced choline acetyltransferase (ChAT) activity in septal neurons and survival of ciliary ganglion neurons. The mRNA expression of CNTF and neurotrophins in BIM cells was differently regulated by the stimulation with cAMP, FGF and retinoic acid. These data suggest multiple regulation and collaboration of neurotrophic factors.

Original languageEnglish
Pages (from-to)763-772
Number of pages10
JournalBiochemistry and Molecular Biology International
Volume38
Issue number4
Publication statusPublished - 1996

Keywords

  • CNTF
  • Choline acetyltransferase
  • FGF
  • Neuroblastoma
  • Neurotrophin
  • Retinoic acid
  • cAMP

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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