Expression of MicroRNA-146 in rheumatoid arthritis synovial tissue

Tomoyuki Nakasa, Shigeru Miyaki, Atsuko Okubo, Megumi Hashimoto, Keiichiro Nishida, Mitsuo Ochi, Hiroshi Asahara

Research output: Contribution to journalArticle

440 Citations (Scopus)

Abstract

Objective. Several microRNA, which are ∼22-nucleotide noncoding RNAs, exhibit tissue-specific or developmental stage-specific expression patterns and are associated with human diseases. The objective of this study was to identify the expression pattern of microRNA-146 (miR-146) in synovial tissue from patients with rheumatoid arthritis (RA). Methods. The expression of miR-146 in synovial tissue from 5 patients with RA, 5 patients with osteoarthritis (OA), and 1 normal subject was analyzed by quantitative reverse transcription- polymerase chain reaction (RT-PCR) and by in situ hybridization and immunohistochemistry of tissue sections. Induction of miR-146 following stimulation with tumor necrosis factor α (TNFα) and interleukin-1β (IL-1β) of cultures of human rheumatoid arthritis synovial fibroblasts (RASFs) was examined by quantitative PCR and RT-PCR. Results. Mature miR-146a and primary miR-146a/b were highly expressed in RA synovial tissue, which also expressed TNFα, but the 2 microRNA were less highly expressed in OA and normal synovial tissue. In situ hybridization showed primary miR-146a expression in cells of the superficial and sublining layers in synovial tissue from RA patients. Cells positive for miR-146a were primarily CD68+ macrophages, but included several CD3+ T cell subsets and CD79a+ B cells. Expression of miR-146a/b was markedly up-regulated in RASFs after stimulation with TNFα and IL-1β. Conclusion. This study shows that miR-146 is expressed in RA synovial tissue and that its expression is induced by stimulation with TNFα and IL-1β. Further studies are required to elucidate the function of miR-146 in these tissues.

Original languageEnglish
Pages (from-to)1284-1292
Number of pages9
JournalArthritis and Rheumatism
Volume58
Issue number5
DOIs
Publication statusPublished - May 2008

Fingerprint

MicroRNAs
Rheumatoid Arthritis
Tumor Necrosis Factor-alpha
Interleukin-1
Osteoarthritis
Polymerase Chain Reaction
Reverse Transcription
In Situ Hybridization
Fibroblasts
Untranslated RNA
T-Lymphocyte Subsets
B-Lymphocytes
Nucleotides
Immunohistochemistry
Macrophages

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Nakasa, T., Miyaki, S., Okubo, A., Hashimoto, M., Nishida, K., Ochi, M., & Asahara, H. (2008). Expression of MicroRNA-146 in rheumatoid arthritis synovial tissue. Arthritis and Rheumatism, 58(5), 1284-1292. https://doi.org/10.1002/art.23429

Expression of MicroRNA-146 in rheumatoid arthritis synovial tissue. / Nakasa, Tomoyuki; Miyaki, Shigeru; Okubo, Atsuko; Hashimoto, Megumi; Nishida, Keiichiro; Ochi, Mitsuo; Asahara, Hiroshi.

In: Arthritis and Rheumatism, Vol. 58, No. 5, 05.2008, p. 1284-1292.

Research output: Contribution to journalArticle

Nakasa, T, Miyaki, S, Okubo, A, Hashimoto, M, Nishida, K, Ochi, M & Asahara, H 2008, 'Expression of MicroRNA-146 in rheumatoid arthritis synovial tissue', Arthritis and Rheumatism, vol. 58, no. 5, pp. 1284-1292. https://doi.org/10.1002/art.23429
Nakasa, Tomoyuki ; Miyaki, Shigeru ; Okubo, Atsuko ; Hashimoto, Megumi ; Nishida, Keiichiro ; Ochi, Mitsuo ; Asahara, Hiroshi. / Expression of MicroRNA-146 in rheumatoid arthritis synovial tissue. In: Arthritis and Rheumatism. 2008 ; Vol. 58, No. 5. pp. 1284-1292.
@article{745f96d7723945f2ae5cc3f0af92c4ce,
title = "Expression of MicroRNA-146 in rheumatoid arthritis synovial tissue",
abstract = "Objective. Several microRNA, which are ∼22-nucleotide noncoding RNAs, exhibit tissue-specific or developmental stage-specific expression patterns and are associated with human diseases. The objective of this study was to identify the expression pattern of microRNA-146 (miR-146) in synovial tissue from patients with rheumatoid arthritis (RA). Methods. The expression of miR-146 in synovial tissue from 5 patients with RA, 5 patients with osteoarthritis (OA), and 1 normal subject was analyzed by quantitative reverse transcription- polymerase chain reaction (RT-PCR) and by in situ hybridization and immunohistochemistry of tissue sections. Induction of miR-146 following stimulation with tumor necrosis factor α (TNFα) and interleukin-1β (IL-1β) of cultures of human rheumatoid arthritis synovial fibroblasts (RASFs) was examined by quantitative PCR and RT-PCR. Results. Mature miR-146a and primary miR-146a/b were highly expressed in RA synovial tissue, which also expressed TNFα, but the 2 microRNA were less highly expressed in OA and normal synovial tissue. In situ hybridization showed primary miR-146a expression in cells of the superficial and sublining layers in synovial tissue from RA patients. Cells positive for miR-146a were primarily CD68+ macrophages, but included several CD3+ T cell subsets and CD79a+ B cells. Expression of miR-146a/b was markedly up-regulated in RASFs after stimulation with TNFα and IL-1β. Conclusion. This study shows that miR-146 is expressed in RA synovial tissue and that its expression is induced by stimulation with TNFα and IL-1β. Further studies are required to elucidate the function of miR-146 in these tissues.",
author = "Tomoyuki Nakasa and Shigeru Miyaki and Atsuko Okubo and Megumi Hashimoto and Keiichiro Nishida and Mitsuo Ochi and Hiroshi Asahara",
year = "2008",
month = "5",
doi = "10.1002/art.23429",
language = "English",
volume = "58",
pages = "1284--1292",
journal = "Arthritis and Rheumatology",
issn = "2326-5191",
publisher = "John Wiley and Sons Ltd",
number = "5",

}

TY - JOUR

T1 - Expression of MicroRNA-146 in rheumatoid arthritis synovial tissue

AU - Nakasa, Tomoyuki

AU - Miyaki, Shigeru

AU - Okubo, Atsuko

AU - Hashimoto, Megumi

AU - Nishida, Keiichiro

AU - Ochi, Mitsuo

AU - Asahara, Hiroshi

PY - 2008/5

Y1 - 2008/5

N2 - Objective. Several microRNA, which are ∼22-nucleotide noncoding RNAs, exhibit tissue-specific or developmental stage-specific expression patterns and are associated with human diseases. The objective of this study was to identify the expression pattern of microRNA-146 (miR-146) in synovial tissue from patients with rheumatoid arthritis (RA). Methods. The expression of miR-146 in synovial tissue from 5 patients with RA, 5 patients with osteoarthritis (OA), and 1 normal subject was analyzed by quantitative reverse transcription- polymerase chain reaction (RT-PCR) and by in situ hybridization and immunohistochemistry of tissue sections. Induction of miR-146 following stimulation with tumor necrosis factor α (TNFα) and interleukin-1β (IL-1β) of cultures of human rheumatoid arthritis synovial fibroblasts (RASFs) was examined by quantitative PCR and RT-PCR. Results. Mature miR-146a and primary miR-146a/b were highly expressed in RA synovial tissue, which also expressed TNFα, but the 2 microRNA were less highly expressed in OA and normal synovial tissue. In situ hybridization showed primary miR-146a expression in cells of the superficial and sublining layers in synovial tissue from RA patients. Cells positive for miR-146a were primarily CD68+ macrophages, but included several CD3+ T cell subsets and CD79a+ B cells. Expression of miR-146a/b was markedly up-regulated in RASFs after stimulation with TNFα and IL-1β. Conclusion. This study shows that miR-146 is expressed in RA synovial tissue and that its expression is induced by stimulation with TNFα and IL-1β. Further studies are required to elucidate the function of miR-146 in these tissues.

AB - Objective. Several microRNA, which are ∼22-nucleotide noncoding RNAs, exhibit tissue-specific or developmental stage-specific expression patterns and are associated with human diseases. The objective of this study was to identify the expression pattern of microRNA-146 (miR-146) in synovial tissue from patients with rheumatoid arthritis (RA). Methods. The expression of miR-146 in synovial tissue from 5 patients with RA, 5 patients with osteoarthritis (OA), and 1 normal subject was analyzed by quantitative reverse transcription- polymerase chain reaction (RT-PCR) and by in situ hybridization and immunohistochemistry of tissue sections. Induction of miR-146 following stimulation with tumor necrosis factor α (TNFα) and interleukin-1β (IL-1β) of cultures of human rheumatoid arthritis synovial fibroblasts (RASFs) was examined by quantitative PCR and RT-PCR. Results. Mature miR-146a and primary miR-146a/b were highly expressed in RA synovial tissue, which also expressed TNFα, but the 2 microRNA were less highly expressed in OA and normal synovial tissue. In situ hybridization showed primary miR-146a expression in cells of the superficial and sublining layers in synovial tissue from RA patients. Cells positive for miR-146a were primarily CD68+ macrophages, but included several CD3+ T cell subsets and CD79a+ B cells. Expression of miR-146a/b was markedly up-regulated in RASFs after stimulation with TNFα and IL-1β. Conclusion. This study shows that miR-146 is expressed in RA synovial tissue and that its expression is induced by stimulation with TNFα and IL-1β. Further studies are required to elucidate the function of miR-146 in these tissues.

UR - http://www.scopus.com/inward/record.url?scp=43949136123&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43949136123&partnerID=8YFLogxK

U2 - 10.1002/art.23429

DO - 10.1002/art.23429

M3 - Article

C2 - 18438844

AN - SCOPUS:43949136123

VL - 58

SP - 1284

EP - 1292

JO - Arthritis and Rheumatology

JF - Arthritis and Rheumatology

SN - 2326-5191

IS - 5

ER -