Expression of MicroRNA-146 in rheumatoid arthritis synovial tissue

Tomoyuki Nakasa, Shigeru Miyaki, Atsuko Okubo, Megumi Hashimoto, Keiichiro Nishida, Mitsuo Ochi, Hiroshi Asahara

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462 Citations (Scopus)

Abstract

Objective. Several microRNA, which are ∼22-nucleotide noncoding RNAs, exhibit tissue-specific or developmental stage-specific expression patterns and are associated with human diseases. The objective of this study was to identify the expression pattern of microRNA-146 (miR-146) in synovial tissue from patients with rheumatoid arthritis (RA). Methods. The expression of miR-146 in synovial tissue from 5 patients with RA, 5 patients with osteoarthritis (OA), and 1 normal subject was analyzed by quantitative reverse transcription- polymerase chain reaction (RT-PCR) and by in situ hybridization and immunohistochemistry of tissue sections. Induction of miR-146 following stimulation with tumor necrosis factor α (TNFα) and interleukin-1β (IL-1β) of cultures of human rheumatoid arthritis synovial fibroblasts (RASFs) was examined by quantitative PCR and RT-PCR. Results. Mature miR-146a and primary miR-146a/b were highly expressed in RA synovial tissue, which also expressed TNFα, but the 2 microRNA were less highly expressed in OA and normal synovial tissue. In situ hybridization showed primary miR-146a expression in cells of the superficial and sublining layers in synovial tissue from RA patients. Cells positive for miR-146a were primarily CD68+ macrophages, but included several CD3+ T cell subsets and CD79a+ B cells. Expression of miR-146a/b was markedly up-regulated in RASFs after stimulation with TNFα and IL-1β. Conclusion. This study shows that miR-146 is expressed in RA synovial tissue and that its expression is induced by stimulation with TNFα and IL-1β. Further studies are required to elucidate the function of miR-146 in these tissues.

Original languageEnglish
Pages (from-to)1284-1292
Number of pages9
JournalArthritis and Rheumatism
Volume58
Issue number5
DOIs
Publication statusPublished - May 2008

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

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    Nakasa, T., Miyaki, S., Okubo, A., Hashimoto, M., Nishida, K., Ochi, M., & Asahara, H. (2008). Expression of MicroRNA-146 in rheumatoid arthritis synovial tissue. Arthritis and Rheumatism, 58(5), 1284-1292. https://doi.org/10.1002/art.23429