Abstract
Ecto-apyrase(s) participates in cell-wall-associated defense through ATP hydrolysis. Here we analyzed Medicago truncatula genes through cDNA screening and in silico analyses against known databases. This study revealed seven genes, five of which (MtAPY1;1 to MtAPY1;5) are members of a legume-specific family, whereas two genes (MtAPY2;1 and MtAPY2;2) are close to those in other plants. Agrobacterium-based transient expression in Nicotiana benthamiana, combined with a c-myc epitope tag technology, confirmed that the MtAPY1;1 is a secreted protein. Transient expression of MtAPY1;1 in leaves of N. benthamiana restricted disease development by a virulent fungus, suggesting a role in disease resistance.
| Original language | English |
|---|---|
| Pages (from-to) | 222-229 |
| Number of pages | 8 |
| Journal | Journal of General Plant Pathology |
| Volume | 80 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - May 2014 |
Keywords
- Ecto-apyrase
- Medicago truncatula
- MtAPY1;1
- Nicotiana benthamiana
- Triple c-myc epitope tag technology
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science
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Toyoda, K., Kawakami, E., Nagai, H., Shiobara-Komatsu, T., Tanaka, K., Inagaki, Y., Ichinose, Y., & Shiraishi, T. (2014). Expression of Medicago truncatula ecto-apyrase MtAPY1;1 in leaves of Nicotiana benthamiana restricts necrotic lesions induced by a virulent fungus. Journal of General Plant Pathology, 80(3), 222-229. https://doi.org/10.1007/s10327-014-0510-x