Expression of Medicago truncatula ecto-apyrase MtAPY1;1 in leaves of Nicotiana benthamiana restricts necrotic lesions induced by a virulent fungus

Kazuhiro Toyoda; Eriko Kawakami; Hideaki Nagai; Taiki Shiobara-Komatsu; Kaori Tanaka; Yoshishige Inagaki; Yuki Ichinose; Tomonori Shiraishi

doi:10.1007/s10327-014-0510-x

Expression of Medicago truncatula ecto-apyrase MtAPY1;1 in leaves of Nicotiana benthamiana restricts necrotic lesions induced by a virulent fungus

Kazuhiro Toyoda, Eriko Kawakami, Hideaki Nagai, Taiki Shiobara-Komatsu, Kaori Tanaka, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Ecto-apyrase(s) participates in cell-wall-associated defense through ATP hydrolysis. Here we analyzed Medicago truncatula genes through cDNA screening and in silico analyses against known databases. This study revealed seven genes, five of which (MtAPY1;1 to MtAPY1;5) are members of a legume-specific family, whereas two genes (MtAPY2;1 and MtAPY2;2) are close to those in other plants. Agrobacterium-based transient expression in Nicotiana benthamiana, combined with a c-myc epitope tag technology, confirmed that the MtAPY1;1 is a secreted protein. Transient expression of MtAPY1;1 in leaves of N. benthamiana restricted disease development by a virulent fungus, suggesting a role in disease resistance.

Original language	English
Pages (from-to)	222-229
Number of pages	8
Journal	Journal of General Plant Pathology
Volume	80
Issue number	3
DOIs	https://doi.org/10.1007/s10327-014-0510-x
Publication status	Published - May 2014

Keywords

Ecto-apyrase
Medicago truncatula
MtAPY1;1
Nicotiana benthamiana
Triple c-myc epitope tag technology

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1007/s10327-014-0510-x

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title = "Expression of Medicago truncatula ecto-apyrase MtAPY1;1 in leaves of Nicotiana benthamiana restricts necrotic lesions induced by a virulent fungus",

abstract = "Ecto-apyrase(s) participates in cell-wall-associated defense through ATP hydrolysis. Here we analyzed Medicago truncatula genes through cDNA screening and in silico analyses against known databases. This study revealed seven genes, five of which (MtAPY1;1 to MtAPY1;5) are members of a legume-specific family, whereas two genes (MtAPY2;1 and MtAPY2;2) are close to those in other plants. Agrobacterium-based transient expression in Nicotiana benthamiana, combined with a c-myc epitope tag technology, confirmed that the MtAPY1;1 is a secreted protein. Transient expression of MtAPY1;1 in leaves of N. benthamiana restricted disease development by a virulent fungus, suggesting a role in disease resistance.",

keywords = "Ecto-apyrase, Medicago truncatula, MtAPY1;1, Nicotiana benthamiana, Triple c-myc epitope tag technology",

author = "Kazuhiro Toyoda and Eriko Kawakami and Hideaki Nagai and Taiki Shiobara-Komatsu and Kaori Tanaka and Yoshishige Inagaki and Yuki Ichinose and Tomonori Shiraishi",

year = "2014",

month = may,

doi = "10.1007/s10327-014-0510-x",

language = "English",

volume = "80",

pages = "222--229",

journal = "Journal of General Plant Pathology",

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T1 - Expression of Medicago truncatula ecto-apyrase MtAPY1;1 in leaves of Nicotiana benthamiana restricts necrotic lesions induced by a virulent fungus

AU - Toyoda, Kazuhiro

AU - Kawakami, Eriko

AU - Nagai, Hideaki

AU - Shiobara-Komatsu, Taiki

AU - Tanaka, Kaori

AU - Inagaki, Yoshishige

AU - Ichinose, Yuki

AU - Shiraishi, Tomonori

PY - 2014/5

Y1 - 2014/5

N2 - Ecto-apyrase(s) participates in cell-wall-associated defense through ATP hydrolysis. Here we analyzed Medicago truncatula genes through cDNA screening and in silico analyses against known databases. This study revealed seven genes, five of which (MtAPY1;1 to MtAPY1;5) are members of a legume-specific family, whereas two genes (MtAPY2;1 and MtAPY2;2) are close to those in other plants. Agrobacterium-based transient expression in Nicotiana benthamiana, combined with a c-myc epitope tag technology, confirmed that the MtAPY1;1 is a secreted protein. Transient expression of MtAPY1;1 in leaves of N. benthamiana restricted disease development by a virulent fungus, suggesting a role in disease resistance.

AB - Ecto-apyrase(s) participates in cell-wall-associated defense through ATP hydrolysis. Here we analyzed Medicago truncatula genes through cDNA screening and in silico analyses against known databases. This study revealed seven genes, five of which (MtAPY1;1 to MtAPY1;5) are members of a legume-specific family, whereas two genes (MtAPY2;1 and MtAPY2;2) are close to those in other plants. Agrobacterium-based transient expression in Nicotiana benthamiana, combined with a c-myc epitope tag technology, confirmed that the MtAPY1;1 is a secreted protein. Transient expression of MtAPY1;1 in leaves of N. benthamiana restricted disease development by a virulent fungus, suggesting a role in disease resistance.

KW - Ecto-apyrase

KW - Medicago truncatula

KW - MtAPY1;1

KW - Nicotiana benthamiana

KW - Triple c-myc epitope tag technology

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DO - 10.1007/s10327-014-0510-x

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EP - 229

JO - Journal of General Plant Pathology

JF - Journal of General Plant Pathology

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ER -

Expression of Medicago truncatula ecto-apyrase MtAPY1;1 in leaves of Nicotiana benthamiana restricts necrotic lesions induced by a virulent fungus

Abstract

Keywords

ASJC Scopus subject areas

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