Abstract
Ecto-apyrase(s) participates in cell-wall-associated defense through ATP hydrolysis. Here we analyzed Medicago truncatula genes through cDNA screening and in silico analyses against known databases. This study revealed seven genes, five of which (MtAPY1;1 to MtAPY1;5) are members of a legume-specific family, whereas two genes (MtAPY2;1 and MtAPY2;2) are close to those in other plants. Agrobacterium-based transient expression in Nicotiana benthamiana, combined with a c-myc epitope tag technology, confirmed that the MtAPY1;1 is a secreted protein. Transient expression of MtAPY1;1 in leaves of N. benthamiana restricted disease development by a virulent fungus, suggesting a role in disease resistance.
| Original language | English |
|---|---|
| Pages (from-to) | 222-229 |
| Number of pages | 8 |
| Journal | Journal of General Plant Pathology |
| Volume | 80 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- Ecto-apyrase
- Medicago truncatula
- MtAPY1;1
- Nicotiana benthamiana
- Triple c-myc epitope tag technology
ASJC Scopus subject areas
- Plant Science
- Agronomy and Crop Science
Cite this
Expression of Medicago truncatula ecto-apyrase MtAPY1;1 in leaves of Nicotiana benthamiana restricts necrotic lesions induced by a virulent fungus. / Toyoda, Kazuhiro; Kawakami, Eriko; Nagai, Hideaki; Shiobara-Komatsu, Taiki; Tanaka, Kaori; Inagaki, Yoshishige; Ichinose, Yuki; Shiraishi, Tomonori.
In: Journal of General Plant Pathology, Vol. 80, No. 3, 2014, p. 222-229.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of Medicago truncatula ecto-apyrase MtAPY1;1 in leaves of Nicotiana benthamiana restricts necrotic lesions induced by a virulent fungus
AU - Toyoda, Kazuhiro
AU - Kawakami, Eriko
AU - Nagai, Hideaki
AU - Shiobara-Komatsu, Taiki
AU - Tanaka, Kaori
AU - Inagaki, Yoshishige
AU - Ichinose, Yuki
AU - Shiraishi, Tomonori
PY - 2014
Y1 - 2014
N2 - Ecto-apyrase(s) participates in cell-wall-associated defense through ATP hydrolysis. Here we analyzed Medicago truncatula genes through cDNA screening and in silico analyses against known databases. This study revealed seven genes, five of which (MtAPY1;1 to MtAPY1;5) are members of a legume-specific family, whereas two genes (MtAPY2;1 and MtAPY2;2) are close to those in other plants. Agrobacterium-based transient expression in Nicotiana benthamiana, combined with a c-myc epitope tag technology, confirmed that the MtAPY1;1 is a secreted protein. Transient expression of MtAPY1;1 in leaves of N. benthamiana restricted disease development by a virulent fungus, suggesting a role in disease resistance.
AB - Ecto-apyrase(s) participates in cell-wall-associated defense through ATP hydrolysis. Here we analyzed Medicago truncatula genes through cDNA screening and in silico analyses against known databases. This study revealed seven genes, five of which (MtAPY1;1 to MtAPY1;5) are members of a legume-specific family, whereas two genes (MtAPY2;1 and MtAPY2;2) are close to those in other plants. Agrobacterium-based transient expression in Nicotiana benthamiana, combined with a c-myc epitope tag technology, confirmed that the MtAPY1;1 is a secreted protein. Transient expression of MtAPY1;1 in leaves of N. benthamiana restricted disease development by a virulent fungus, suggesting a role in disease resistance.
KW - Ecto-apyrase
KW - Medicago truncatula
KW - MtAPY1;1
KW - Nicotiana benthamiana
KW - Triple c-myc epitope tag technology
UR - http://www.scopus.com/inward/record.url?scp=84901239384&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901239384&partnerID=8YFLogxK
U2 - 10.1007/s10327-014-0510-x
DO - 10.1007/s10327-014-0510-x
M3 - Article
VL - 80
SP - 222
EP - 229
JO - Journal of General Plant Pathology
JF - Journal of General Plant Pathology
SN - 1345-2630
IS - 3
ER -