Expression of LOX-1 in spontaneously hypertensive rat carotid atherosclerotic lesions

Y. Hamakawa, N. Omori, K. Sato, I. Nagano, M. Shoji, Koji Abe

Research output: Contribution to journalArticle

Abstract

Oxidized low-density lipoprotein (Ox-LDL) has been implicated in the pathogenesis of atherosclerosis (Proc. Natl. Acad. Sci. U. S. A. 84 (1987) 5928, N. Engl. J. Med. 320 (1989) 915, J. Clin. Invest. 88 (1991) 1785). It is hypothesized that Ox-LDL is trapped by a scavenger receptor on the surface of macrophages, resulting in the formation of foam cells and subsequent atherosclerotic lesion (Nature (Lond.) 343 (1990) 531). Ox-LDL is also reported to be internalized and degraded in the endothelium, attenuates the endothelium-dependent vasodilatory response through reduced production of nitric oxide (NO), and induces endothelial expression of leucocyte adhesion molecules (J. Clin. Invest. 90 (1992) 1138) and smooth-muscle growth factors (Nature 362 (1993) 801). In this study, we examined the vascular expression of lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) in hypercholesterolaemic SHR-SP/Izm rats by immunohistochemistry. Hypercholesterolaemic SHR-SP/Izm rats were produced by feeding SHR-SP/Izm a high fat and high cholesterol diet (H.F.C.) containing 0.023% NG-nitro-L-arginine (L-NNA) and 1% NaCl in their drinking water (Clin. Exp. Pharmacol. Physiol. 24 (1997) 344). We found that LOX-1 expression was low in the common carotid artery of WKY/Izm, whereas it was markedly up-regulated in those of SHR-SP/Izm rats and hypercholesterolaemic SHR-SP/Izm rats. These results indicated that LOX-1 expression in the carotid artery were up-regulated not only in hypertensive rats but in hypercholesterolaemic rats, which may be involved in the impaired endothelium-dependent vasodilatation in these rats.

Original languageEnglish
Pages (from-to)209-212
Number of pages4
JournalInternational Congress Series
Volume1252
Issue numberC
DOIs
Publication statusPublished - Jun 1 2003

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Inbred SHR Rats
Endothelium
Class E Scavenger Receptors
Scavenger Receptors
Foam Cells
Nitroarginine
Common Carotid Artery
Cell Adhesion Molecules
High Fat Diet
Carotid Arteries
Vasodilation
Drinking Water
Smooth Muscle
Blood Vessels
Atherosclerosis
Intercellular Signaling Peptides and Proteins
Nitric Oxide
Immunohistochemistry
Macrophages
Cholesterol

Keywords

  • Atherosclerosis
  • Carotid artery
  • Hypercholesterolaemic stroke-prone spontaneously hypertensive rats (SHR-SP/Izm)
  • Lectin-like oxidized low density lipoprotein receptor-1 (LOX-1)
  • Oxidized low-density lipoprotein (Ox-LDL)

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Expression of LOX-1 in spontaneously hypertensive rat carotid atherosclerotic lesions. / Hamakawa, Y.; Omori, N.; Sato, K.; Nagano, I.; Shoji, M.; Abe, Koji.

In: International Congress Series, Vol. 1252, No. C, 01.06.2003, p. 209-212.

Research output: Contribution to journalArticle

Hamakawa, Y. ; Omori, N. ; Sato, K. ; Nagano, I. ; Shoji, M. ; Abe, Koji. / Expression of LOX-1 in spontaneously hypertensive rat carotid atherosclerotic lesions. In: International Congress Series. 2003 ; Vol. 1252, No. C. pp. 209-212.
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AU - Abe, Koji

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AB - Oxidized low-density lipoprotein (Ox-LDL) has been implicated in the pathogenesis of atherosclerosis (Proc. Natl. Acad. Sci. U. S. A. 84 (1987) 5928, N. Engl. J. Med. 320 (1989) 915, J. Clin. Invest. 88 (1991) 1785). It is hypothesized that Ox-LDL is trapped by a scavenger receptor on the surface of macrophages, resulting in the formation of foam cells and subsequent atherosclerotic lesion (Nature (Lond.) 343 (1990) 531). Ox-LDL is also reported to be internalized and degraded in the endothelium, attenuates the endothelium-dependent vasodilatory response through reduced production of nitric oxide (NO), and induces endothelial expression of leucocyte adhesion molecules (J. Clin. Invest. 90 (1992) 1138) and smooth-muscle growth factors (Nature 362 (1993) 801). In this study, we examined the vascular expression of lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) in hypercholesterolaemic SHR-SP/Izm rats by immunohistochemistry. Hypercholesterolaemic SHR-SP/Izm rats were produced by feeding SHR-SP/Izm a high fat and high cholesterol diet (H.F.C.) containing 0.023% NG-nitro-L-arginine (L-NNA) and 1% NaCl in their drinking water (Clin. Exp. Pharmacol. Physiol. 24 (1997) 344). We found that LOX-1 expression was low in the common carotid artery of WKY/Izm, whereas it was markedly up-regulated in those of SHR-SP/Izm rats and hypercholesterolaemic SHR-SP/Izm rats. These results indicated that LOX-1 expression in the carotid artery were up-regulated not only in hypertensive rats but in hypercholesterolaemic rats, which may be involved in the impaired endothelium-dependent vasodilatation in these rats.

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