Expression of L-histidine decarboxylase in mouse male germ cells

Faizah Safina, Satoshi Tanaka, Mariko Inagaki, Kazuhito Tsuboi, Yukihiko Sugimoto, Atsushi Ichikawa

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Histamine synthesis in male reproductive tissues remains largely unknown. The interaction between stem cell factor and its receptor, c-Kit, has been found to be essential for the maturation of male germ cells and peripheral mast cells. Based on this analogy, we investigated the expression of histidine decarboxylase (HDC), the rate-limiting enzyme of histamine synthesis, in mouse male germ cells. Immunohistochemical analyses revealed that HDC is localized in the acrosomes of spermatids and spermatozoa. In the testis, epididymis, and spermatozoa, a significant amount of histamine and HDC activity were detected. W/Wv mice, known to lack most of their germ cells in the seminiferous tubules, were found to lack HDC protein expression as well as HDC activity in the testis. An in vitro acrosome reaction induced by a calcium ionophore, A23187, caused the release of histamine from epididymal spermatozoa. Our observations indicate that histamine is produced in and released from the acrosomes.

Original languageEnglish
Pages (from-to)14211-14215
Number of pages5
JournalJournal of Biological Chemistry
Volume277
Issue number16
DOIs
Publication statusPublished - Apr 19 2002
Externally publishedYes

Fingerprint

Histidine Decarboxylase
Histidine
Germ Cells
Histamine
Cells
Spermatozoa
Acrosome
Testis
Proto-Oncogene Proteins c-kit
Acrosome Reaction
Seminiferous Tubules
Spermatids
Calcium Ionophores
Epididymis
Histamine Release
Calcimycin
Mast Cells
Tissue
Enzymes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Safina, F., Tanaka, S., Inagaki, M., Tsuboi, K., Sugimoto, Y., & Ichikawa, A. (2002). Expression of L-histidine decarboxylase in mouse male germ cells. Journal of Biological Chemistry, 277(16), 14211-14215. https://doi.org/10.1074/jbc.M200702200

Expression of L-histidine decarboxylase in mouse male germ cells. / Safina, Faizah; Tanaka, Satoshi; Inagaki, Mariko; Tsuboi, Kazuhito; Sugimoto, Yukihiko; Ichikawa, Atsushi.

In: Journal of Biological Chemistry, Vol. 277, No. 16, 19.04.2002, p. 14211-14215.

Research output: Contribution to journalArticle

Safina, F, Tanaka, S, Inagaki, M, Tsuboi, K, Sugimoto, Y & Ichikawa, A 2002, 'Expression of L-histidine decarboxylase in mouse male germ cells', Journal of Biological Chemistry, vol. 277, no. 16, pp. 14211-14215. https://doi.org/10.1074/jbc.M200702200
Safina F, Tanaka S, Inagaki M, Tsuboi K, Sugimoto Y, Ichikawa A. Expression of L-histidine decarboxylase in mouse male germ cells. Journal of Biological Chemistry. 2002 Apr 19;277(16):14211-14215. https://doi.org/10.1074/jbc.M200702200
Safina, Faizah ; Tanaka, Satoshi ; Inagaki, Mariko ; Tsuboi, Kazuhito ; Sugimoto, Yukihiko ; Ichikawa, Atsushi. / Expression of L-histidine decarboxylase in mouse male germ cells. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 16. pp. 14211-14215.
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