Expression of junctional adhesion molecules on the human lymphatic endothelium

Takeshi Ueki, Kana Iwasawa, Hiroyuki Ishikawa, Yoshihiko Sawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Human lymphatic vessels express several leukocyte adhesion molecules. The study here investigated the expression of three junctional adhesion molecules (JAM) which are a newly reported glycoprotein family of adhesion molecules on human lymphatic endothelium. In this study, JAM-1 and JAM-3 but not JAM-2 were detected in cultured human neonatal dermal lymphatic endothelial cells (LEC) at the gene and protein levels by microarray, RT-PCR, real-time PCR, and immunohistochemical analysis. The JAM-1 and JAM-3 expression was not altered in the TNF-α-treated LEC or in the untreated cells. In human tissue, the expression of JAM-1, and the expression of JAM-1, JAM-2, and JAM-3 were observed in collecting lymphatic vessels of uninflamed small intestine, and in initial lymphatics of inflamed tongue and uninflamed gingival tissue. It is thought that JAM-2 mRNA could be produced in mature vascular endothelium but not in cultured cells, and that human intestinal and oral lymphatic vessels usually express JAM-1, JAM-2, and JAM-3. There were initial lymphatics simultaneously expressing JAM-1, JAM-2, and JAM-3 in the mucosal connective tissue papillae of gingival tissue. The three JAM expressions on the lymphatic endothelium may contribute to both seal the cell-cell contact at interendothelial junctions and also allow lymphocytes to transmigrate into lymphatic vessels from tissue, independent of inflammatory cytokines.

Original languageEnglish
Pages (from-to)269-278
Number of pages10
JournalMicrovascular Research
Volume75
Issue number2
DOIs
Publication statusPublished - Mar 2008
Externally publishedYes

Fingerprint

Junctional Adhesion Molecules
Lymphatic Endothelium
Junctional Adhesion Molecule B
Junctional Adhesion Molecule C
Lymphatic Vessels
Tissue
Endothelial cells
Endothelial Cells
Protein Array Analysis
Lymphocytes
Vascular Endothelium
Cell Adhesion Molecules
Lymphoid Tissue
Microarrays
Tongue
Connective Tissue
Small Intestine
Seals
Real-Time Polymerase Chain Reaction
Cultured Cells

Keywords

  • JAM-1
  • JAM-2
  • JAM-3
  • Lymphatic endothelium
  • TNF-α

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

Expression of junctional adhesion molecules on the human lymphatic endothelium. / Ueki, Takeshi; Iwasawa, Kana; Ishikawa, Hiroyuki; Sawa, Yoshihiko.

In: Microvascular Research, Vol. 75, No. 2, 03.2008, p. 269-278.

Research output: Contribution to journalArticle

Ueki, Takeshi ; Iwasawa, Kana ; Ishikawa, Hiroyuki ; Sawa, Yoshihiko. / Expression of junctional adhesion molecules on the human lymphatic endothelium. In: Microvascular Research. 2008 ; Vol. 75, No. 2. pp. 269-278.
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