Expression of insulin-like growth factor I gene in osmoregulatory organs during seawater adaptation of the salmonid fish

Possible mode of osmoregulatory action of growth hormone

Tatsuya Sakamoto, Tetsuya Hirano

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Growth hormone has been shown to contribute to seawater adaptation of salmonid fishes. The growth influence of growth hormone is mediated largely by hepatic production of insulin-like growth factor I (IGF-I). To study the growth hormone-IGF-I axis in osmoregulation, we measured IGF-I mRNA in the liver, gill, and body kidney from rainbow trout by Northern analysis. The levels of IGF-I mRNA in all tissues increased significantly after injection of growth hormone. Transfer of trout from fresh water to 80% seawater evoked an increase in plasma growth hormone after 1 day. IGF-I mRNA was not altered significantly in the liver, but it was increased in the gill and body kidney after 1 and 8 days, respectively. These observations indicate that the IGF-I gene is expressed differently among these organs during seawater adaptation. Growth hormone may stimulate hypoosmoregulatory ability by inducing local expression of IGF-I in osmoregulatory organs, although the possibility that IGF-I expression might occur in part independently of growth hormone during seawater adaptation cannot be excluded.

Original languageEnglish
Pages (from-to)1912-1916
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number5
Publication statusPublished - Mar 1 1993
Externally publishedYes

Fingerprint

Seawater
Insulin-Like Growth Factor I
Growth Hormone
Fishes
Genes
Messenger RNA
Liver
Kidney
Osmoregulation
Trout
Oncorhynchus mykiss
Fresh Water
Injections
Growth

Keywords

  • Gene regulation
  • Gill
  • Kidney
  • Rainbow trout
  • RNA hybridization

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

@article{260b75ae49f64d78adee7fdefbdddcef,
title = "Expression of insulin-like growth factor I gene in osmoregulatory organs during seawater adaptation of the salmonid fish: Possible mode of osmoregulatory action of growth hormone",
abstract = "Growth hormone has been shown to contribute to seawater adaptation of salmonid fishes. The growth influence of growth hormone is mediated largely by hepatic production of insulin-like growth factor I (IGF-I). To study the growth hormone-IGF-I axis in osmoregulation, we measured IGF-I mRNA in the liver, gill, and body kidney from rainbow trout by Northern analysis. The levels of IGF-I mRNA in all tissues increased significantly after injection of growth hormone. Transfer of trout from fresh water to 80{\%} seawater evoked an increase in plasma growth hormone after 1 day. IGF-I mRNA was not altered significantly in the liver, but it was increased in the gill and body kidney after 1 and 8 days, respectively. These observations indicate that the IGF-I gene is expressed differently among these organs during seawater adaptation. Growth hormone may stimulate hypoosmoregulatory ability by inducing local expression of IGF-I in osmoregulatory organs, although the possibility that IGF-I expression might occur in part independently of growth hormone during seawater adaptation cannot be excluded.",
keywords = "Gene regulation, Gill, Kidney, Rainbow trout, RNA hybridization",
author = "Tatsuya Sakamoto and Tetsuya Hirano",
year = "1993",
month = "3",
day = "1",
language = "English",
volume = "90",
pages = "1912--1916",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "5",

}

TY - JOUR

T1 - Expression of insulin-like growth factor I gene in osmoregulatory organs during seawater adaptation of the salmonid fish

T2 - Possible mode of osmoregulatory action of growth hormone

AU - Sakamoto, Tatsuya

AU - Hirano, Tetsuya

PY - 1993/3/1

Y1 - 1993/3/1

N2 - Growth hormone has been shown to contribute to seawater adaptation of salmonid fishes. The growth influence of growth hormone is mediated largely by hepatic production of insulin-like growth factor I (IGF-I). To study the growth hormone-IGF-I axis in osmoregulation, we measured IGF-I mRNA in the liver, gill, and body kidney from rainbow trout by Northern analysis. The levels of IGF-I mRNA in all tissues increased significantly after injection of growth hormone. Transfer of trout from fresh water to 80% seawater evoked an increase in plasma growth hormone after 1 day. IGF-I mRNA was not altered significantly in the liver, but it was increased in the gill and body kidney after 1 and 8 days, respectively. These observations indicate that the IGF-I gene is expressed differently among these organs during seawater adaptation. Growth hormone may stimulate hypoosmoregulatory ability by inducing local expression of IGF-I in osmoregulatory organs, although the possibility that IGF-I expression might occur in part independently of growth hormone during seawater adaptation cannot be excluded.

AB - Growth hormone has been shown to contribute to seawater adaptation of salmonid fishes. The growth influence of growth hormone is mediated largely by hepatic production of insulin-like growth factor I (IGF-I). To study the growth hormone-IGF-I axis in osmoregulation, we measured IGF-I mRNA in the liver, gill, and body kidney from rainbow trout by Northern analysis. The levels of IGF-I mRNA in all tissues increased significantly after injection of growth hormone. Transfer of trout from fresh water to 80% seawater evoked an increase in plasma growth hormone after 1 day. IGF-I mRNA was not altered significantly in the liver, but it was increased in the gill and body kidney after 1 and 8 days, respectively. These observations indicate that the IGF-I gene is expressed differently among these organs during seawater adaptation. Growth hormone may stimulate hypoosmoregulatory ability by inducing local expression of IGF-I in osmoregulatory organs, although the possibility that IGF-I expression might occur in part independently of growth hormone during seawater adaptation cannot be excluded.

KW - Gene regulation

KW - Gill

KW - Kidney

KW - Rainbow trout

KW - RNA hybridization

UR - http://www.scopus.com/inward/record.url?scp=0027512549&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027512549&partnerID=8YFLogxK

M3 - Article

VL - 90

SP - 1912

EP - 1916

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 5

ER -