Expression of IL-8 during reperfusion of renal allografts is dependent on ischemic time

Motoo Araki, Nader Fahmy, Lingmei Zhou, Hiromi Kumon, Venkatesh Krishnamurthi, David Goldfarb, Charles Modlin, Stuart Flechner, Andrew C. Novick, Robert L. Fairchild

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background. Ischemia/reperfusion injury is an inherent consequence of solid organ transplantation that increases tissue inflammation and negatively impacts organ transplant function and survival. This study investigated the expression levels of chemokine and chemokine receptor genes in living versus cadaver donor renal allografts before and after reperfusion. Methods. This study involved 39 renal transplant patients (19 cadaveric and 20 living donor). The ischemia biopsy was taken just before graft declamping and the reperfusion biopsy 30 min after declamping. Whole-cell RNA was isolated and chemokine (IL-8, CCL2/MCP-1, CXCL10/IP-10 and CCL5/RANTES) and chemokine receptor (CCR2 and CCR5) expression was tested by quantitative PCR. Results. Just prior to declamping, ischemic cadaveric donor grafts had higher expression of CXCL10/IP-10 but not IL-8 or CCL2/MCP-1 than living donor grafts. IL-8 expression increased 50% from ischemia to reperfusion in living donor grafts but increased more than 13-fold during reperfusion of cadaver donor grafts. Increased total ischemia time induced greater IL-8 expression during reperfusion. MCP-1 expression also increased during reperfusion of living and cadaver donor grafts but differences were not observed between the two groups of grafts. RANTES, CCR2, and CCR5 expression did not change in ischemic vs. reperfusion biopsies. Conclusions. The expression of chemokines directing neutrophil and macrophage recruitment increases during reperfusion of living and cadaveric donor renal allografts. Expression levels of IL-8 correlate with the ischemic time imposed on the renal graft. Early tissue injury may be attenuated by strategies antagonizing chemokines directing the recruitment of neutrophils and macrophages into kidney grafts.

Original languageEnglish
Pages (from-to)783-788
Number of pages6
JournalTransplantation
Volume81
Issue number5
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Interleukin-8
Reperfusion
Allografts
Transplants
Kidney
Living Donors
Chemokines
Cadaver
Chemokine CCL5
Ischemia
Neutrophil Infiltration
Chemokine Receptors
Tissue Donors
Biopsy
Macrophages
Tissue Survival
Organ Transplantation
Reperfusion Injury
RNA
Inflammation

Keywords

  • Chemokines
  • Inflammation
  • Interleukin-8
  • Ischemia/reperfusion
  • Renal graft

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Araki, M., Fahmy, N., Zhou, L., Kumon, H., Krishnamurthi, V., Goldfarb, D., ... Fairchild, R. L. (2006). Expression of IL-8 during reperfusion of renal allografts is dependent on ischemic time. Transplantation, 81(5), 783-788. https://doi.org/10.1097/01.tp.0000198736.69527.32

Expression of IL-8 during reperfusion of renal allografts is dependent on ischemic time. / Araki, Motoo; Fahmy, Nader; Zhou, Lingmei; Kumon, Hiromi; Krishnamurthi, Venkatesh; Goldfarb, David; Modlin, Charles; Flechner, Stuart; Novick, Andrew C.; Fairchild, Robert L.

In: Transplantation, Vol. 81, No. 5, 03.2006, p. 783-788.

Research output: Contribution to journalArticle

Araki, M, Fahmy, N, Zhou, L, Kumon, H, Krishnamurthi, V, Goldfarb, D, Modlin, C, Flechner, S, Novick, AC & Fairchild, RL 2006, 'Expression of IL-8 during reperfusion of renal allografts is dependent on ischemic time', Transplantation, vol. 81, no. 5, pp. 783-788. https://doi.org/10.1097/01.tp.0000198736.69527.32
Araki, Motoo ; Fahmy, Nader ; Zhou, Lingmei ; Kumon, Hiromi ; Krishnamurthi, Venkatesh ; Goldfarb, David ; Modlin, Charles ; Flechner, Stuart ; Novick, Andrew C. ; Fairchild, Robert L. / Expression of IL-8 during reperfusion of renal allografts is dependent on ischemic time. In: Transplantation. 2006 ; Vol. 81, No. 5. pp. 783-788.
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abstract = "Background. Ischemia/reperfusion injury is an inherent consequence of solid organ transplantation that increases tissue inflammation and negatively impacts organ transplant function and survival. This study investigated the expression levels of chemokine and chemokine receptor genes in living versus cadaver donor renal allografts before and after reperfusion. Methods. This study involved 39 renal transplant patients (19 cadaveric and 20 living donor). The ischemia biopsy was taken just before graft declamping and the reperfusion biopsy 30 min after declamping. Whole-cell RNA was isolated and chemokine (IL-8, CCL2/MCP-1, CXCL10/IP-10 and CCL5/RANTES) and chemokine receptor (CCR2 and CCR5) expression was tested by quantitative PCR. Results. Just prior to declamping, ischemic cadaveric donor grafts had higher expression of CXCL10/IP-10 but not IL-8 or CCL2/MCP-1 than living donor grafts. IL-8 expression increased 50{\%} from ischemia to reperfusion in living donor grafts but increased more than 13-fold during reperfusion of cadaver donor grafts. Increased total ischemia time induced greater IL-8 expression during reperfusion. MCP-1 expression also increased during reperfusion of living and cadaver donor grafts but differences were not observed between the two groups of grafts. RANTES, CCR2, and CCR5 expression did not change in ischemic vs. reperfusion biopsies. Conclusions. The expression of chemokines directing neutrophil and macrophage recruitment increases during reperfusion of living and cadaveric donor renal allografts. Expression levels of IL-8 correlate with the ischemic time imposed on the renal graft. Early tissue injury may be attenuated by strategies antagonizing chemokines directing the recruitment of neutrophils and macrophages into kidney grafts.",
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AU - Araki, Motoo

AU - Fahmy, Nader

AU - Zhou, Lingmei

AU - Kumon, Hiromi

AU - Krishnamurthi, Venkatesh

AU - Goldfarb, David

AU - Modlin, Charles

AU - Flechner, Stuart

AU - Novick, Andrew C.

AU - Fairchild, Robert L.

PY - 2006/3

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N2 - Background. Ischemia/reperfusion injury is an inherent consequence of solid organ transplantation that increases tissue inflammation and negatively impacts organ transplant function and survival. This study investigated the expression levels of chemokine and chemokine receptor genes in living versus cadaver donor renal allografts before and after reperfusion. Methods. This study involved 39 renal transplant patients (19 cadaveric and 20 living donor). The ischemia biopsy was taken just before graft declamping and the reperfusion biopsy 30 min after declamping. Whole-cell RNA was isolated and chemokine (IL-8, CCL2/MCP-1, CXCL10/IP-10 and CCL5/RANTES) and chemokine receptor (CCR2 and CCR5) expression was tested by quantitative PCR. Results. Just prior to declamping, ischemic cadaveric donor grafts had higher expression of CXCL10/IP-10 but not IL-8 or CCL2/MCP-1 than living donor grafts. IL-8 expression increased 50% from ischemia to reperfusion in living donor grafts but increased more than 13-fold during reperfusion of cadaver donor grafts. Increased total ischemia time induced greater IL-8 expression during reperfusion. MCP-1 expression also increased during reperfusion of living and cadaver donor grafts but differences were not observed between the two groups of grafts. RANTES, CCR2, and CCR5 expression did not change in ischemic vs. reperfusion biopsies. Conclusions. The expression of chemokines directing neutrophil and macrophage recruitment increases during reperfusion of living and cadaveric donor renal allografts. Expression levels of IL-8 correlate with the ischemic time imposed on the renal graft. Early tissue injury may be attenuated by strategies antagonizing chemokines directing the recruitment of neutrophils and macrophages into kidney grafts.

AB - Background. Ischemia/reperfusion injury is an inherent consequence of solid organ transplantation that increases tissue inflammation and negatively impacts organ transplant function and survival. This study investigated the expression levels of chemokine and chemokine receptor genes in living versus cadaver donor renal allografts before and after reperfusion. Methods. This study involved 39 renal transplant patients (19 cadaveric and 20 living donor). The ischemia biopsy was taken just before graft declamping and the reperfusion biopsy 30 min after declamping. Whole-cell RNA was isolated and chemokine (IL-8, CCL2/MCP-1, CXCL10/IP-10 and CCL5/RANTES) and chemokine receptor (CCR2 and CCR5) expression was tested by quantitative PCR. Results. Just prior to declamping, ischemic cadaveric donor grafts had higher expression of CXCL10/IP-10 but not IL-8 or CCL2/MCP-1 than living donor grafts. IL-8 expression increased 50% from ischemia to reperfusion in living donor grafts but increased more than 13-fold during reperfusion of cadaver donor grafts. Increased total ischemia time induced greater IL-8 expression during reperfusion. MCP-1 expression also increased during reperfusion of living and cadaver donor grafts but differences were not observed between the two groups of grafts. RANTES, CCR2, and CCR5 expression did not change in ischemic vs. reperfusion biopsies. Conclusions. The expression of chemokines directing neutrophil and macrophage recruitment increases during reperfusion of living and cadaveric donor renal allografts. Expression levels of IL-8 correlate with the ischemic time imposed on the renal graft. Early tissue injury may be attenuated by strategies antagonizing chemokines directing the recruitment of neutrophils and macrophages into kidney grafts.

KW - Chemokines

KW - Inflammation

KW - Interleukin-8

KW - Ischemia/reperfusion

KW - Renal graft

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