Expression of human immunoglobulin E ε chain cDNA in E.coli

Tsutornu Kurokawa, Masaharu Seno, Reiko Sasada, Yoshitaka Ona, Haruo Onda, Koichi Igarashi, Masakazu Kikuchi, Yukio Sugino, Tasuku Honjo

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26 Citations (Scopus)

Abstract

Using the cDNA of human 1D6DC; chain, three expression plasmids that code directly the constant portion of the 1D6DC; chain (C1D6DC;l-C 1D6DC;4, C 1D6DC;2-C 1D6DC;C4 and C 1D6DC;3-C 1D6DC;4 domains) were constructed. These 1D6DC; chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human 1D6DC; chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids.

Original languageEnglish
Pages (from-to)3077-3085
Number of pages9
JournalNucleic acids research
Volume11
Issue number10
DOIs
Publication statusPublished - May 25 1983
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Kurokawa, T., Seno, M., Sasada, R., Ona, Y., Onda, H., Igarashi, K., Kikuchi, M., Sugino, Y., & Honjo, T. (1983). Expression of human immunoglobulin E ε chain cDNA in E.coli. Nucleic acids research, 11(10), 3077-3085. https://doi.org/10.1093/nar/11.10.3077