TY - JOUR
T1 - Expression of human immunoglobulin E ε chain cDNA in E.coli
AU - Kurokawa, Tsutornu
AU - Seno, Masaharu
AU - Sasada, Reiko
AU - Ona, Yoshitaka
AU - Onda, Haruo
AU - Igarashi, Koichi
AU - Kikuchi, Masakazu
AU - Sugino, Yukio
AU - Honjo, Tasuku
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 1983/5/25
Y1 - 1983/5/25
N2 - Using the cDNA of human 1D6DC; chain, three expression plasmids that code directly the constant portion of the 1D6DC; chain (C1D6DC;l-C 1D6DC;4, C 1D6DC;2-C 1D6DC;C4 and C 1D6DC;3-C 1D6DC;4 domains) were constructed. These 1D6DC; chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human 1D6DC; chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids.
AB - Using the cDNA of human 1D6DC; chain, three expression plasmids that code directly the constant portion of the 1D6DC; chain (C1D6DC;l-C 1D6DC;4, C 1D6DC;2-C 1D6DC;C4 and C 1D6DC;3-C 1D6DC;4 domains) were constructed. These 1D6DC; chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human 1D6DC; chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids.
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U2 - 10.1093/nar/11.10.3077
DO - 10.1093/nar/11.10.3077
M3 - Article
C2 - 6304621
AN - SCOPUS:0021112016
VL - 11
SP - 3077
EP - 3085
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 10
ER -