Expression of human immunoglobulin E ε chain cDNA in E.coli

Tsutornu Kurokawa, Masaharu Seno, Reiko Sasada, Yoshitaka Ona, Haruo Onda, Koichi Igarashi, Masakazu Kikuchi, Yukio Sugino, Tasuku Honjo

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Using the cDNA of human 1D6DC; chain, three expression plasmids that code directly the constant portion of the 1D6DC; chain (C1D6DC;l-C 1D6DC;4, C 1D6DC;2-C 1D6DC;C4 and C 1D6DC;3-C 1D6DC;4 domains) were constructed. These 1D6DC; chain peptides were synthesized in E. coli under the control of the trp promoter-operator. The bacterially produced peptides have the antigenicity of human 1D6DC; chain and gave the molecular weights equal to the values calculated from the amino acid sequence of the constructed plasmids.

Original languageEnglish
Pages (from-to)3077-3085
Number of pages9
JournalNucleic acids research.
Volume11
Issue number10
DOIs
Publication statusPublished - May 25 1983
Externally publishedYes

Fingerprint

Immunoglobulin Subunits
Immunoglobulin
CDNA
Escherichia coli
Immunoglobulin E
Escherichia Coli
Peptides
Plasmids
Complementary DNA
Amino acids
Amino Acid Sequence
Molecular Weight
Molecular weight
Amino Acids
Promoter
Human
cDNA
Operator

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Kurokawa, T., Seno, M., Sasada, R., Ona, Y., Onda, H., Igarashi, K., ... Honjo, T. (1983). Expression of human immunoglobulin E ε chain cDNA in E.coli. Nucleic acids research., 11(10), 3077-3085. https://doi.org/10.1093/nar/11.10.3077

Expression of human immunoglobulin E ε chain cDNA in E.coli. / Kurokawa, Tsutornu; Seno, Masaharu; Sasada, Reiko; Ona, Yoshitaka; Onda, Haruo; Igarashi, Koichi; Kikuchi, Masakazu; Sugino, Yukio; Honjo, Tasuku.

In: Nucleic acids research., Vol. 11, No. 10, 25.05.1983, p. 3077-3085.

Research output: Contribution to journalArticle

Kurokawa, T, Seno, M, Sasada, R, Ona, Y, Onda, H, Igarashi, K, Kikuchi, M, Sugino, Y & Honjo, T 1983, 'Expression of human immunoglobulin E ε chain cDNA in E.coli', Nucleic acids research., vol. 11, no. 10, pp. 3077-3085. https://doi.org/10.1093/nar/11.10.3077
Kurokawa T, Seno M, Sasada R, Ona Y, Onda H, Igarashi K et al. Expression of human immunoglobulin E ε chain cDNA in E.coli. Nucleic acids research. 1983 May 25;11(10):3077-3085. https://doi.org/10.1093/nar/11.10.3077
Kurokawa, Tsutornu ; Seno, Masaharu ; Sasada, Reiko ; Ona, Yoshitaka ; Onda, Haruo ; Igarashi, Koichi ; Kikuchi, Masakazu ; Sugino, Yukio ; Honjo, Tasuku. / Expression of human immunoglobulin E ε chain cDNA in E.coli. In: Nucleic acids research. 1983 ; Vol. 11, No. 10. pp. 3077-3085.
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