Expression of human α- and β-defensins in odontogenic keratocytes and radicular cysts

Tomohito Yoshimoto, Nobuyoshi Mizukawa, Koichi Sawaki, Makoto Nakano, Eiki Yamachika, Toshio Sugahara, Tomoichiro Yamaai

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: To identify the location of human β-defensin-2 and human α-defensins in odontogenic keratocysts and radicular cysts. Patients and Methods: Immunohistochemical staining of tissue sections from enucleation specimens of 14 patients with odontogenic keratocysts and 19 patients with radicular cysts, and from normal oral mucosa from 2 healthy volunteers was done using mouse monoclonal antibodies against human cytokeratin 5/6 and rabbit polyclonal antibodies against human β-defensin-2. Results: Human β-defensin-2 immunoreactivity was evident in 10 of 14 odontogenic keratocysts (72%), and in 7 of 19 radicular cysts (37%). Signals for human β-defensin-2 were detected in the cytoplasm of keratinocytes in the parakeratinised and granular layers in both types of cysts. The localisation of human β-defensin-2 did not differ between cysts and normal mucosa. Human α-defensin immunoreactivity was detected in both types of cysts, but not in keratinocytes. Signals for human α-defensins were detected in the cytoplasm of neutrophils in the lamina propria. Conclusion: Many cysts generate human β-defensin-2 and human α-defensins suggesting that similar defence mechanisms utilising defensins exist in both types of cysts, as well as in normal oral mucosa.

Original languageEnglish
Pages (from-to)242-247
Number of pages6
JournalAsian Journal of Oral and Maxillofacial Surgery
Volume16
Issue number4
Publication statusPublished - 2004

Fingerprint

Radicular Cyst
Odontogenic Cysts
Defensins
Cysts
Mouth Mucosa
Keratinocytes
Cytoplasm
Mucous Membrane
Keratin-6
Keratin-5

Keywords

  • Alpha-Defensins
  • Beta-defensin-2
  • Odontogenic cysts
  • Radicular cyst

ASJC Scopus subject areas

  • Surgery

Cite this

Expression of human α- and β-defensins in odontogenic keratocytes and radicular cysts. / Yoshimoto, Tomohito; Mizukawa, Nobuyoshi; Sawaki, Koichi; Nakano, Makoto; Yamachika, Eiki; Sugahara, Toshio; Yamaai, Tomoichiro.

In: Asian Journal of Oral and Maxillofacial Surgery, Vol. 16, No. 4, 2004, p. 242-247.

Research output: Contribution to journalArticle

Yoshimoto, Tomohito ; Mizukawa, Nobuyoshi ; Sawaki, Koichi ; Nakano, Makoto ; Yamachika, Eiki ; Sugahara, Toshio ; Yamaai, Tomoichiro. / Expression of human α- and β-defensins in odontogenic keratocytes and radicular cysts. In: Asian Journal of Oral and Maxillofacial Surgery. 2004 ; Vol. 16, No. 4. pp. 242-247.
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AB - Objective: To identify the location of human β-defensin-2 and human α-defensins in odontogenic keratocysts and radicular cysts. Patients and Methods: Immunohistochemical staining of tissue sections from enucleation specimens of 14 patients with odontogenic keratocysts and 19 patients with radicular cysts, and from normal oral mucosa from 2 healthy volunteers was done using mouse monoclonal antibodies against human cytokeratin 5/6 and rabbit polyclonal antibodies against human β-defensin-2. Results: Human β-defensin-2 immunoreactivity was evident in 10 of 14 odontogenic keratocysts (72%), and in 7 of 19 radicular cysts (37%). Signals for human β-defensin-2 were detected in the cytoplasm of keratinocytes in the parakeratinised and granular layers in both types of cysts. The localisation of human β-defensin-2 did not differ between cysts and normal mucosa. Human α-defensin immunoreactivity was detected in both types of cysts, but not in keratinocytes. Signals for human α-defensins were detected in the cytoplasm of neutrophils in the lamina propria. Conclusion: Many cysts generate human β-defensin-2 and human α-defensins suggesting that similar defence mechanisms utilising defensins exist in both types of cysts, as well as in normal oral mucosa.

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