Abstract
Objective: To identify the location of human β-defensin-2 and human α-defensins in odontogenic keratocysts and radicular cysts. Patients and Methods: Immunohistochemical staining of tissue sections from enucleation specimens of 14 patients with odontogenic keratocysts and 19 patients with radicular cysts, and from normal oral mucosa from 2 healthy volunteers was done using mouse monoclonal antibodies against human cytokeratin 5/6 and rabbit polyclonal antibodies against human β-defensin-2. Results: Human β-defensin-2 immunoreactivity was evident in 10 of 14 odontogenic keratocysts (72%), and in 7 of 19 radicular cysts (37%). Signals for human β-defensin-2 were detected in the cytoplasm of keratinocytes in the parakeratinised and granular layers in both types of cysts. The localisation of human β-defensin-2 did not differ between cysts and normal mucosa. Human α-defensin immunoreactivity was detected in both types of cysts, but not in keratinocytes. Signals for human α-defensins were detected in the cytoplasm of neutrophils in the lamina propria. Conclusion: Many cysts generate human β-defensin-2 and human α-defensins suggesting that similar defence mechanisms utilising defensins exist in both types of cysts, as well as in normal oral mucosa.
Original language | English |
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Pages (from-to) | 242-247 |
Number of pages | 6 |
Journal | Asian Journal of Oral and Maxillofacial Surgery |
Volume | 16 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2004 |
Keywords
- Alpha-Defensins
- Beta-defensin-2
- Odontogenic cysts
- Radicular cyst
ASJC Scopus subject areas
- Surgery
- Oral Surgery
- Orthopedics and Sports Medicine