Abstract
Objective: To identify the location of human β-defensin-2 and human α-defensins in odontogenic keratocysts and radicular cysts. Patients and Methods: Immunohistochemical staining of tissue sections from enucleation specimens of 14 patients with odontogenic keratocysts and 19 patients with radicular cysts, and from normal oral mucosa from 2 healthy volunteers was done using mouse monoclonal antibodies against human cytokeratin 5/6 and rabbit polyclonal antibodies against human β-defensin-2. Results: Human β-defensin-2 immunoreactivity was evident in 10 of 14 odontogenic keratocysts (72%), and in 7 of 19 radicular cysts (37%). Signals for human β-defensin-2 were detected in the cytoplasm of keratinocytes in the parakeratinised and granular layers in both types of cysts. The localisation of human β-defensin-2 did not differ between cysts and normal mucosa. Human α-defensin immunoreactivity was detected in both types of cysts, but not in keratinocytes. Signals for human α-defensins were detected in the cytoplasm of neutrophils in the lamina propria. Conclusion: Many cysts generate human β-defensin-2 and human α-defensins suggesting that similar defence mechanisms utilising defensins exist in both types of cysts, as well as in normal oral mucosa.
| Original language | English |
|---|---|
| Pages (from-to) | 242-247 |
| Number of pages | 6 |
| Journal | Asian Journal of Oral and Maxillofacial Surgery |
| Volume | 16 |
| Issue number | 4 |
| Publication status | Published - 2004 |
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Keywords
- Alpha-Defensins
- Beta-defensin-2
- Odontogenic cysts
- Radicular cyst
ASJC Scopus subject areas
- Surgery
Cite this
Expression of human α- and β-defensins in odontogenic keratocytes and radicular cysts. / Yoshimoto, Tomohito; Mizukawa, Nobuyoshi; Sawaki, Koichi; Nakano, Makoto; Yamachika, Eiki; Sugahara, Toshio; Yamaai, Tomoichiro.
In: Asian Journal of Oral and Maxillofacial Surgery, Vol. 16, No. 4, 2004, p. 242-247.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of human α- and β-defensins in odontogenic keratocytes and radicular cysts
AU - Yoshimoto, Tomohito
AU - Mizukawa, Nobuyoshi
AU - Sawaki, Koichi
AU - Nakano, Makoto
AU - Yamachika, Eiki
AU - Sugahara, Toshio
AU - Yamaai, Tomoichiro
PY - 2004
Y1 - 2004
N2 - Objective: To identify the location of human β-defensin-2 and human α-defensins in odontogenic keratocysts and radicular cysts. Patients and Methods: Immunohistochemical staining of tissue sections from enucleation specimens of 14 patients with odontogenic keratocysts and 19 patients with radicular cysts, and from normal oral mucosa from 2 healthy volunteers was done using mouse monoclonal antibodies against human cytokeratin 5/6 and rabbit polyclonal antibodies against human β-defensin-2. Results: Human β-defensin-2 immunoreactivity was evident in 10 of 14 odontogenic keratocysts (72%), and in 7 of 19 radicular cysts (37%). Signals for human β-defensin-2 were detected in the cytoplasm of keratinocytes in the parakeratinised and granular layers in both types of cysts. The localisation of human β-defensin-2 did not differ between cysts and normal mucosa. Human α-defensin immunoreactivity was detected in both types of cysts, but not in keratinocytes. Signals for human α-defensins were detected in the cytoplasm of neutrophils in the lamina propria. Conclusion: Many cysts generate human β-defensin-2 and human α-defensins suggesting that similar defence mechanisms utilising defensins exist in both types of cysts, as well as in normal oral mucosa.
AB - Objective: To identify the location of human β-defensin-2 and human α-defensins in odontogenic keratocysts and radicular cysts. Patients and Methods: Immunohistochemical staining of tissue sections from enucleation specimens of 14 patients with odontogenic keratocysts and 19 patients with radicular cysts, and from normal oral mucosa from 2 healthy volunteers was done using mouse monoclonal antibodies against human cytokeratin 5/6 and rabbit polyclonal antibodies against human β-defensin-2. Results: Human β-defensin-2 immunoreactivity was evident in 10 of 14 odontogenic keratocysts (72%), and in 7 of 19 radicular cysts (37%). Signals for human β-defensin-2 were detected in the cytoplasm of keratinocytes in the parakeratinised and granular layers in both types of cysts. The localisation of human β-defensin-2 did not differ between cysts and normal mucosa. Human α-defensin immunoreactivity was detected in both types of cysts, but not in keratinocytes. Signals for human α-defensins were detected in the cytoplasm of neutrophils in the lamina propria. Conclusion: Many cysts generate human β-defensin-2 and human α-defensins suggesting that similar defence mechanisms utilising defensins exist in both types of cysts, as well as in normal oral mucosa.
KW - Alpha-Defensins
KW - Beta-defensin-2
KW - Odontogenic cysts
KW - Radicular cyst
UR - http://www.scopus.com/inward/record.url?scp=12344262007&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12344262007&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:12344262007
VL - 16
SP - 242
EP - 247
JO - Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
JF - Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
SN - 2212-5558
IS - 4
ER -