Abstract
A mouse monoclonal antibody (MoAb), MG-2, was produced by immunizing a characterized human megakaryoblastic cell line, MEG-01. Since MG-2 reacted with erythrocytes of all ABH blood groups except O(h) (O Bombay), and since anti-H MoAb inhibited MG-2 binding to MEG-01 cells, MG-2 is considered to recognize a molecule closely related to blood group antigen H. MG-2 reacted more strongly with normal smaller sized megakaryocytes than with larger sized ones, and not with platelets. The expression of the intrinsic H-related antigen on MEG-01 cells decreased concomitant to megakaryocytic differentiation induced by phorbol esters. This H-related antigen was expressed on leukemia cells with the megakaryocytic features from blast crisis of chronic myelogenous leukemia and acute megakaryoblastic leukemia.
| Original language | English |
|---|---|
| Pages (from-to) | 151-158 |
| Number of pages | 8 |
| Journal | International Journal of Hematology |
| Volume | 54 |
| Issue number | 2 |
| Publication status | Published - 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Hematology
Cite this
Expression of H-related antigen on human megakaryocytes and megakaryocytic leukemic cells. / Okumura, M.; Morishima, Y.; Ogura, M.; Morishita, Y.; Tanimoto, M.; Ohno, R.; Saito, H.
In: International Journal of Hematology, Vol. 54, No. 2, 1991, p. 151-158.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of H-related antigen on human megakaryocytes and megakaryocytic leukemic cells
AU - Okumura, M.
AU - Morishima, Y.
AU - Ogura, M.
AU - Morishita, Y.
AU - Tanimoto, M.
AU - Ohno, R.
AU - Saito, H.
PY - 1991
Y1 - 1991
N2 - A mouse monoclonal antibody (MoAb), MG-2, was produced by immunizing a characterized human megakaryoblastic cell line, MEG-01. Since MG-2 reacted with erythrocytes of all ABH blood groups except O(h) (O Bombay), and since anti-H MoAb inhibited MG-2 binding to MEG-01 cells, MG-2 is considered to recognize a molecule closely related to blood group antigen H. MG-2 reacted more strongly with normal smaller sized megakaryocytes than with larger sized ones, and not with platelets. The expression of the intrinsic H-related antigen on MEG-01 cells decreased concomitant to megakaryocytic differentiation induced by phorbol esters. This H-related antigen was expressed on leukemia cells with the megakaryocytic features from blast crisis of chronic myelogenous leukemia and acute megakaryoblastic leukemia.
AB - A mouse monoclonal antibody (MoAb), MG-2, was produced by immunizing a characterized human megakaryoblastic cell line, MEG-01. Since MG-2 reacted with erythrocytes of all ABH blood groups except O(h) (O Bombay), and since anti-H MoAb inhibited MG-2 binding to MEG-01 cells, MG-2 is considered to recognize a molecule closely related to blood group antigen H. MG-2 reacted more strongly with normal smaller sized megakaryocytes than with larger sized ones, and not with platelets. The expression of the intrinsic H-related antigen on MEG-01 cells decreased concomitant to megakaryocytic differentiation induced by phorbol esters. This H-related antigen was expressed on leukemia cells with the megakaryocytic features from blast crisis of chronic myelogenous leukemia and acute megakaryoblastic leukemia.
UR - http://www.scopus.com/inward/record.url?scp=0025758827&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025758827&partnerID=8YFLogxK
M3 - Article
VL - 54
SP - 151
EP - 158
JO - International Journal of Hematology
JF - International Journal of Hematology
SN - 0925-5710
IS - 2
ER -