Expression of H-related antigen on human megakaryocytes and megakaryocytic leukemic cells

M. Okumura; Y. Morishima; M. Ogura; Y. Morishita; M. Tanimoto; R. Ohno; H. Saito

Expression of H-related antigen on human megakaryocytes and megakaryocytic leukemic cells

M. Okumura, Y. Morishima, M. Ogura, Y. Morishita, M. Tanimoto, R. Ohno, H. Saito

Graduate School of Medicine Dentistry and Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A mouse monoclonal antibody (MoAb), MG-2, was produced by immunizing a characterized human megakaryoblastic cell line, MEG-01. Since MG-2 reacted with erythrocytes of all ABH blood groups except O(h) (O Bombay), and since anti-H MoAb inhibited MG-2 binding to MEG-01 cells, MG-2 is considered to recognize a molecule closely related to blood group antigen H. MG-2 reacted more strongly with normal smaller sized megakaryocytes than with larger sized ones, and not with platelets. The expression of the intrinsic H-related antigen on MEG-01 cells decreased concomitant to megakaryocytic differentiation induced by phorbol esters. This H-related antigen was expressed on leukemia cells with the megakaryocytic features from blast crisis of chronic myelogenous leukemia and acute megakaryoblastic leukemia.

Original language	English
Pages (from-to)	151-158
Number of pages	8
Journal	International journal of hematology
Volume	54
Issue number	2
Publication status	Published - 1991

ASJC Scopus subject areas

Hematology

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title = "Expression of H-related antigen on human megakaryocytes and megakaryocytic leukemic cells",

abstract = "A mouse monoclonal antibody (MoAb), MG-2, was produced by immunizing a characterized human megakaryoblastic cell line, MEG-01. Since MG-2 reacted with erythrocytes of all ABH blood groups except O(h) (O Bombay), and since anti-H MoAb inhibited MG-2 binding to MEG-01 cells, MG-2 is considered to recognize a molecule closely related to blood group antigen H. MG-2 reacted more strongly with normal smaller sized megakaryocytes than with larger sized ones, and not with platelets. The expression of the intrinsic H-related antigen on MEG-01 cells decreased concomitant to megakaryocytic differentiation induced by phorbol esters. This H-related antigen was expressed on leukemia cells with the megakaryocytic features from blast crisis of chronic myelogenous leukemia and acute megakaryoblastic leukemia.",

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year = "1991",

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T1 - Expression of H-related antigen on human megakaryocytes and megakaryocytic leukemic cells

AU - Okumura, M.

AU - Morishima, Y.

AU - Ogura, M.

AU - Morishita, Y.

AU - Tanimoto, M.

AU - Ohno, R.

AU - Saito, H.

PY - 1991

Y1 - 1991

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AB - A mouse monoclonal antibody (MoAb), MG-2, was produced by immunizing a characterized human megakaryoblastic cell line, MEG-01. Since MG-2 reacted with erythrocytes of all ABH blood groups except O(h) (O Bombay), and since anti-H MoAb inhibited MG-2 binding to MEG-01 cells, MG-2 is considered to recognize a molecule closely related to blood group antigen H. MG-2 reacted more strongly with normal smaller sized megakaryocytes than with larger sized ones, and not with platelets. The expression of the intrinsic H-related antigen on MEG-01 cells decreased concomitant to megakaryocytic differentiation induced by phorbol esters. This H-related antigen was expressed on leukemia cells with the megakaryocytic features from blast crisis of chronic myelogenous leukemia and acute megakaryoblastic leukemia.

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Expression of H-related antigen on human megakaryocytes and megakaryocytic leukemic cells

Abstract

ASJC Scopus subject areas

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