Expression of G protein-coupled receptor 30 in the spinal somatosensory system

Keiko Takanami, Hirotaka Sakamoto, Ken Ichi Matsuda, Koji Hosokawa, Mayumi Nishi, Eric R. Prossnitz, Mitsuhiro Kawata

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Estrogens were originally identified as the primary sex steroid hormones in females and regulators of reproductive function and sexual behavior, but it has long been suggested that estrogens also have local effects on the somatosensory system at the spinal cord level. It is well known that the effects of estrogens are mediated by nuclear estrogen receptors (ERs) through genomic action, but recently a membrane-bound G protein-coupled receptor, GPR30, was identified as a non-genomic estrogen receptor. In this study we investigated the presence and localization of GPR30 in the rat spinal cord and dorsal root ganglion (DRG) in comparison with ERα. Using immunohistochemistry and in situ hybridization, we showed the expression of GPR30 in DRG neurons in male and female rats at mRNA and protein levels without specific sexual difference. A dense accumulation of GPR30 immunoreactivity was observed in the outer layer of the spinal dorsal horn, and selective spinal dorsal rhizotomy revealed that GPR30 was transported from the DRG to terminals located in the spinal dorsal horn. GPR30 expression was downregulated in DRG neurons of ovariectomized female rats. The spinal somatosensory system might be modulated by estradiol via putative membrane ER, GPR30-mediated mechanism.

Original languageEnglish
Pages (from-to)17-28
Number of pages12
JournalBrain Research
Volume1310
DOIs
Publication statusPublished - Jan 15 2010
Externally publishedYes

Fingerprint

Spinal Ganglia
G-Protein-Coupled Receptors
Estrogen Receptors
Estrogens
Spinal Cord
Rhizotomy
Neurons
Reproductive Behavior
Membranes
Spinal Nerve Roots
Gonadal Steroid Hormones
Sexual Behavior
In Situ Hybridization
Estradiol
Down-Regulation
Immunohistochemistry
Messenger RNA
Proteins
Spinal Cord Dorsal Horn

Keywords

  • Dorsal root ganglion
  • Estrogen
  • Estrogen receptor α
  • GPR30
  • Somatosensory neuron system
  • Spinal cord

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Developmental Biology
  • Molecular Biology

Cite this

Takanami, K., Sakamoto, H., Matsuda, K. I., Hosokawa, K., Nishi, M., Prossnitz, E. R., & Kawata, M. (2010). Expression of G protein-coupled receptor 30 in the spinal somatosensory system. Brain Research, 1310, 17-28. https://doi.org/10.1016/j.brainres.2009.11.004

Expression of G protein-coupled receptor 30 in the spinal somatosensory system. / Takanami, Keiko; Sakamoto, Hirotaka; Matsuda, Ken Ichi; Hosokawa, Koji; Nishi, Mayumi; Prossnitz, Eric R.; Kawata, Mitsuhiro.

In: Brain Research, Vol. 1310, 15.01.2010, p. 17-28.

Research output: Contribution to journalArticle

Takanami, K, Sakamoto, H, Matsuda, KI, Hosokawa, K, Nishi, M, Prossnitz, ER & Kawata, M 2010, 'Expression of G protein-coupled receptor 30 in the spinal somatosensory system', Brain Research, vol. 1310, pp. 17-28. https://doi.org/10.1016/j.brainres.2009.11.004
Takanami, Keiko ; Sakamoto, Hirotaka ; Matsuda, Ken Ichi ; Hosokawa, Koji ; Nishi, Mayumi ; Prossnitz, Eric R. ; Kawata, Mitsuhiro. / Expression of G protein-coupled receptor 30 in the spinal somatosensory system. In: Brain Research. 2010 ; Vol. 1310. pp. 17-28.
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