Expression of functional Na+/H+ antiporters of Helicobacter pylori in antiporter-deficient Echerichia coli mutants

Hiroki Inoue, Tatsuya Sakurai, Satoshi Ujike, Tomofusa Tsuchiya, Hiroshi Murakami, Hiroshi Kanazawa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

An open reading frame with a sequence homologous to Escherichia coli Na+/H+ antiporter A (ENhaA) was found in the total genomic sequence of Helicobacter Pylori, a pathogenic bacterium of gastric inflammation, and was named HNhaA. The primary sequences and the hydropathy profiles of ENhaA and HNhaA were very homologous except for one additional region found in HNhaA. This sequence has about 40 hydrophilic amino acid residues inserted at the position next to residue 235 of ENhaA which corresponds to residue 245 of HNhaA. HNhaA was expressed in E. coli mutants deficient in Na+/H+ antiporters and complemented the salt-sensitive phenotype of the mutants. Membrane vesicles prepared from these transformants of HNhaA using mutants deficient in the antiporters had the antiporter activities. Surprisingly, the antiporter activity in the transformant membranes was high at acidic and neutral pH, while ENhaA did not function at these pHs. A hydrophilic region around residue 235 in ENhaA and the additional hydrophilic region of about 40 residues in the same region found in HNhaA might be responsible for this difference in activity by acting as putative pH sensors.

Original languageEnglish
Pages (from-to)11-16
Number of pages6
JournalFEBS Letters
Volume443
Issue number1
DOIs
Publication statusPublished - Jan 22 1999

Fingerprint

Antiporters
Sodium-Hydrogen Antiporter
Helicobacter pylori
Escherichia coli
pH sensors
Membranes
Sequence Homology
Open Reading Frames
Stomach
Bacteria
Salts
Inflammation
Phenotype
Amino Acids

Keywords

  • Escherichia coli
  • Helicobacter pylori
  • Na/H antiporter

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression of functional Na+/H+ antiporters of Helicobacter pylori in antiporter-deficient Echerichia coli mutants. / Inoue, Hiroki; Sakurai, Tatsuya; Ujike, Satoshi; Tsuchiya, Tomofusa; Murakami, Hiroshi; Kanazawa, Hiroshi.

In: FEBS Letters, Vol. 443, No. 1, 22.01.1999, p. 11-16.

Research output: Contribution to journalArticle

Inoue, Hiroki ; Sakurai, Tatsuya ; Ujike, Satoshi ; Tsuchiya, Tomofusa ; Murakami, Hiroshi ; Kanazawa, Hiroshi. / Expression of functional Na+/H+ antiporters of Helicobacter pylori in antiporter-deficient Echerichia coli mutants. In: FEBS Letters. 1999 ; Vol. 443, No. 1. pp. 11-16.
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