Expression of functional acetylcholine receptor from cloned cDNAs

Masayoshi Mishina, Tomohiro Kurosaki, Takamasa Tobimatsu, Yuuki Morimoto, Masaharu Noda, Tokuo Yamamoto, Mineko Terao, Jon Lindstrom, Tomoyuki Takahashi, Motoy Kuno, Shosaku Numa

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Abstract

The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.

Original languageEnglish
Pages (from-to)604-608
Number of pages5
JournalNature
Volume307
Issue number5952
DOIs
Publication statusPublished - 1984
Externally publishedYes

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Mishina, M., Kurosaki, T., Tobimatsu, T., Morimoto, Y., Noda, M., Yamamoto, T., Terao, M., Lindstrom, J., Takahashi, T., Kuno, M., & Numa, S. (1984). Expression of functional acetylcholine receptor from cloned cDNAs. Nature, 307(5952), 604-608. https://doi.org/10.1038/307604a0