Expression of functional acetylcholine receptor from cloned cDNAs

Masayoshi Mishina, Tomohiro Kurosaki, Takamasa Tobimatsu, Yuuki Morimoto, Masaharu Noda, Tokuo Yamamoto, Mineko Terao, Jon Lindstrom, Tomoyuki Takahashi, Motoy Kuno, Shosaku Numa

Research output: Contribution to journalArticle

239 Citations (Scopus)

Abstract

The cloned cDNAs encoding the four subunits of the Torpedo californica acetylcholine receptor, each carried by a simian virus 40 vector, direct the synthesis of the functional receptor in a combined expression system consisting of COS monkey cells and Xenopus oocytes. Our results suggest that all four subunits are required to elicit a normal nicotinic response to acetylcholine, whereas only the α-subunit is indispensable for α-bungarotoxin binding activity.

Original languageEnglish
Pages (from-to)604-608
Number of pages5
JournalNature
Volume307
Issue number5952
DOIs
Publication statusPublished - 1984
Externally publishedYes

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Torpedo
Bungarotoxins
Simian virus 40
COS Cells
Cholinergic Receptors
Xenopus
Acetylcholine
Oocytes
Haplorhini
Complementary DNA

ASJC Scopus subject areas

  • General

Cite this

Mishina, M., Kurosaki, T., Tobimatsu, T., Morimoto, Y., Noda, M., Yamamoto, T., ... Numa, S. (1984). Expression of functional acetylcholine receptor from cloned cDNAs. Nature, 307(5952), 604-608. https://doi.org/10.1038/307604a0

Expression of functional acetylcholine receptor from cloned cDNAs. / Mishina, Masayoshi; Kurosaki, Tomohiro; Tobimatsu, Takamasa; Morimoto, Yuuki; Noda, Masaharu; Yamamoto, Tokuo; Terao, Mineko; Lindstrom, Jon; Takahashi, Tomoyuki; Kuno, Motoy; Numa, Shosaku.

In: Nature, Vol. 307, No. 5952, 1984, p. 604-608.

Research output: Contribution to journalArticle

Mishina, M, Kurosaki, T, Tobimatsu, T, Morimoto, Y, Noda, M, Yamamoto, T, Terao, M, Lindstrom, J, Takahashi, T, Kuno, M & Numa, S 1984, 'Expression of functional acetylcholine receptor from cloned cDNAs', Nature, vol. 307, no. 5952, pp. 604-608. https://doi.org/10.1038/307604a0
Mishina M, Kurosaki T, Tobimatsu T, Morimoto Y, Noda M, Yamamoto T et al. Expression of functional acetylcholine receptor from cloned cDNAs. Nature. 1984;307(5952):604-608. https://doi.org/10.1038/307604a0
Mishina, Masayoshi ; Kurosaki, Tomohiro ; Tobimatsu, Takamasa ; Morimoto, Yuuki ; Noda, Masaharu ; Yamamoto, Tokuo ; Terao, Mineko ; Lindstrom, Jon ; Takahashi, Tomoyuki ; Kuno, Motoy ; Numa, Shosaku. / Expression of functional acetylcholine receptor from cloned cDNAs. In: Nature. 1984 ; Vol. 307, No. 5952. pp. 604-608.
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