Expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) during fracture healing

Eiji Nakada, T. Nakanishi, A. Kawai, K. Asaumi, T. Yamaai, M. Asano, Takashi Nishida, S. Mitani, H. Inoue, Masaharu Takigawa

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Localization and expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) during fracture healing in mouse ribs were investigated. In situ hybridization demonstrated that CTGF/Hcs24 mRNA was remarkably expressed, especially in hypertrophic chondrocytes and proliferating chondrocytes, in the regions of regenerating cartilage on days 8 and 14 after fracture. CTGF/Hcs24 mRNA was also expressed in proliferating periosteal cells in the vicinity of the fracture sites on days 2 and 8, and in cells in fibrous tissue around the callus on day 8. Northern blot analysis showed that expression of CTGF/Hcs24 mRNA was 3.9 times higher on day 2 of fracture healing than that on day 0. On day 8, it reached a peak of 8.6 times higher than that on day 0. It then declined to a lower level. Immunostaining showed that CTGF/Hcs24 was localized in hypertrophic chondrocytes and proliferating chondrocytes in the regions of regenerating cartilage, and in active osteoblasts in the regions of intramembranous ossification. Although CTGF/Hcs24 was abundant in the proliferating and differentiating cells (on days 8 and 14), immunostaining decreased as the cells differentiated to form bone (on day 20). CTGF/Hcs24 was also detected in cells in fibrous tissue, vascular endothelial cells in the callus, and periosteal cells around the fracture sites. These results suggest that CTGF/Hcs24 plays some role in fracture healing.

Original languageEnglish
Pages (from-to)441-447
Number of pages7
JournalBone
Volume31
Issue number4
DOIs
Publication statusPublished - Oct 1 2002

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Connective Tissue Growth Factor
Fracture Healing
Chondrocytes
Genes
Bony Callus
Messenger RNA
Cartilage
Ribs
Osteoblasts
Osteogenesis
Northern Blotting
In Situ Hybridization

Keywords

  • Connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24)
  • Endochondral ossification
  • Fracture healing
  • Immunostaining
  • In situ hybridization
  • Intramembranous ossification

ASJC Scopus subject areas

  • Physiology
  • Hematology

Cite this

Expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) during fracture healing. / Nakada, Eiji; Nakanishi, T.; Kawai, A.; Asaumi, K.; Yamaai, T.; Asano, M.; Nishida, Takashi; Mitani, S.; Inoue, H.; Takigawa, Masaharu.

In: Bone, Vol. 31, No. 4, 01.10.2002, p. 441-447.

Research output: Contribution to journalArticle

Nakada, Eiji ; Nakanishi, T. ; Kawai, A. ; Asaumi, K. ; Yamaai, T. ; Asano, M. ; Nishida, Takashi ; Mitani, S. ; Inoue, H. ; Takigawa, Masaharu. / Expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) during fracture healing. In: Bone. 2002 ; Vol. 31, No. 4. pp. 441-447.
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AU - Yamaai, T.

AU - Asano, M.

AU - Nishida, Takashi

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AU - Inoue, H.

AU - Takigawa, Masaharu

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AB - Localization and expression of connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) during fracture healing in mouse ribs were investigated. In situ hybridization demonstrated that CTGF/Hcs24 mRNA was remarkably expressed, especially in hypertrophic chondrocytes and proliferating chondrocytes, in the regions of regenerating cartilage on days 8 and 14 after fracture. CTGF/Hcs24 mRNA was also expressed in proliferating periosteal cells in the vicinity of the fracture sites on days 2 and 8, and in cells in fibrous tissue around the callus on day 8. Northern blot analysis showed that expression of CTGF/Hcs24 mRNA was 3.9 times higher on day 2 of fracture healing than that on day 0. On day 8, it reached a peak of 8.6 times higher than that on day 0. It then declined to a lower level. Immunostaining showed that CTGF/Hcs24 was localized in hypertrophic chondrocytes and proliferating chondrocytes in the regions of regenerating cartilage, and in active osteoblasts in the regions of intramembranous ossification. Although CTGF/Hcs24 was abundant in the proliferating and differentiating cells (on days 8 and 14), immunostaining decreased as the cells differentiated to form bone (on day 20). CTGF/Hcs24 was also detected in cells in fibrous tissue, vascular endothelial cells in the callus, and periosteal cells around the fracture sites. These results suggest that CTGF/Hcs24 plays some role in fracture healing.

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