Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus

Takehiko Mima, Kazuyoshi Goto, Yumiko Yamamoto, Keiko Maeda, Taku Shirakawa, Shunsuke Matsui, Yumi Murata, Takaki Koide, Hiroshi Tokumitsu, Osamu Matsushita

Research output: Contribution to journalArticle

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Abstract

Vibrio alginolyticus is an opportunistic pathogen in both humans and marine animals. Collagenase encoded by colA is considered to be one of the virulence factors. Expression of colA is regulated by multiple environmental factors, e.g., temperature, growth phase, and substrate. To elucidate the mechanism of regulation of colA expression, transposon mutagenesis was performed. VarS, a sensor histidine kinase of the two-component regulatory system, was demonstrated to regulate the expression of colA. VarA, a cognate response regulator of VarS, was also identified and shown to be involved in the regulation of colA expression. In vitro phosphorylation assays showed that phosphorylated VarS acted as a phosphoryl group donor to VarA. A site-directed mutagenesis study showed that the His300, Asp718 and His874 residues in VarS were essential for the phosphorylation of VarS, and the Asp54 residue in VarA was likely to receive the phosphoryl group from VarS. The results demonstrate that the VarS/VarA two-component regulatory system regulates the expression of collagenase in V. alginolyticus.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalJournal of Membrane Biology
DOIs
Publication statusAccepted/In press - Oct 9 2017

Fingerprint

Vibrio alginolyticus
Colicins
Collagenases
Phosphorylation
Virulence Factors
Site-Directed Mutagenesis
Mutagenesis
Temperature
Growth

Keywords

  • Collagenase
  • Two-component regulatory system
  • Vibrio alginolyticus

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

Cite this

Expression of Collagenase is Regulated by the VarS/VarA Two-Component Regulatory System in Vibrio alginolyticus. / Mima, Takehiko; Goto, Kazuyoshi; Yamamoto, Yumiko; Maeda, Keiko; Shirakawa, Taku; Matsui, Shunsuke; Murata, Yumi; Koide, Takaki; Tokumitsu, Hiroshi; Matsushita, Osamu.

In: Journal of Membrane Biology, 09.10.2017, p. 1-13.

Research output: Contribution to journalArticle

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AU - Shirakawa, Taku

AU - Matsui, Shunsuke

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