Expression of cDNA encoding human basic fibroblast growth factor in E.coli

Makoto Iwane, Tsutomu Kurokawa, Reiko Sasada, Masaharu Seno, Shizue Nakagawa, Koichi Igarashi

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Abstract

The cDNA encoding human basic fibroblast growth factor was expressed in E.coli under the control of trp promoter. Bacterially synthesized hbFGF was highly purified using a heparin affinity HPLC column. By this chromatography, hbFGF was eluted as four distinct forms, which were indistinguishable by SDS polyacrylamide gel electrophoresis, amino acid composition, and partial terminal sequence analysis. These molecules stimulated the growth of fibroblasts and endothelial cells although their specific activities varied. The angiogenesis activity of these molecules was also confirmed.

Original languageEnglish
Pages (from-to)470-477
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume146
Issue number2
DOIs
Publication statusPublished - Jul 31 1987

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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