Expression of cDNA encoding human basic fibroblast growth factor in E.coli

Makoto Iwane, Tsutomu Kurokawa, Reiko Sasada, Masaharu Seno, Shizue Nakagawa, Koichi Igarashi

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The cDNA encoding human basic fibroblast growth factor was expressed in E.coli under the control of trp promoter. Bacterially synthesized hbFGF was highly purified using a heparin affinity HPLC column. By this chromatography, hbFGF was eluted as four distinct forms, which were indistinguishable by SDS polyacrylamide gel electrophoresis, amino acid composition, and partial terminal sequence analysis. These molecules stimulated the growth of fibroblasts and endothelial cells although their specific activities varied. The angiogenesis activity of these molecules was also confirmed.

Original languageEnglish
Pages (from-to)470-477
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume146
Issue number2
DOIs
Publication statusPublished - Jul 31 1987
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 2
Escherichia coli
Sequence Analysis
Heparin
Chromatography
Polyacrylamide Gel Electrophoresis
Endothelial Cells
Complementary DNA
Fibroblasts
High Pressure Liquid Chromatography
Amino Acids
Molecules
Endothelial cells
Growth
Electrophoresis
Chemical analysis
polyacrylamide gels

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression of cDNA encoding human basic fibroblast growth factor in E.coli. / Iwane, Makoto; Kurokawa, Tsutomu; Sasada, Reiko; Seno, Masaharu; Nakagawa, Shizue; Igarashi, Koichi.

In: Biochemical and Biophysical Research Communications, Vol. 146, No. 2, 31.07.1987, p. 470-477.

Research output: Contribution to journalArticle

Iwane, Makoto ; Kurokawa, Tsutomu ; Sasada, Reiko ; Seno, Masaharu ; Nakagawa, Shizue ; Igarashi, Koichi. / Expression of cDNA encoding human basic fibroblast growth factor in E.coli. In: Biochemical and Biophysical Research Communications. 1987 ; Vol. 146, No. 2. pp. 470-477.
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