Expression of caspase-3 and -9 relevant to cartilage destruction and chondrocyte apoptosis in human osteoarthritic cartilage.

M. Matsuo, Keiichiro Nishida, A. Yoshida, T. Murakami, H. Inoue

Research output: Contribution to journalArticle

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Abstract

To clarify the involvement of the caspase family in the pathway of NO-induced chondrocyte apoptosis, osteoarthritis (OA) cartilage obtained from 8 patients undergoing total hip arthroplasty were used for histopathological study. Cartilage samples taken from non-fibrillated areas of femoral head resected during surgery for femoral neck fracture were used for comparison. DNA fragmentation of chondrocytes was detected by the nick end-labeling (TUNEL) method. Apoptosis was further confirmed by transmission electron microscopy. The distributions of nitrotyrosine (NT), caspase-3, and -9 were examined immunohistochemically. The populations of apoptotic as well as NT-, caspase-3-, and -9-positive cells were quantified by counting the number of cells in the superficial, middle, and deep layers, respectively. The TUNEL-positive cells were observed primarily in superficial proliferating chondrocytes, clustering chondrocytes, and deep-layer chondrocytes of OA cartilage. Few positive cells were seen in the proliferating chondrocytes in the middle layer. Positive reactions for caspase-3 and -9 were observed in chondrocytes in similar areas. Histological OA grade showed significant correlations with the mean populations of apoptotic chondrocytes (% apoptosis) over the 3 areas. The populations of NT-positive cells (% NT) over the same areas also showed significant correlation with OA grade. Positivity for caspase-3 closely correlated with the OA grade, % apoptosis and %NT. It was concluded that caspase-3 and -9 could play a role in NO-induced chondrocyte apoptosis in OA cartilage.

Original languageEnglish
Pages (from-to)333-340
Number of pages8
JournalActa Medica Okayama
Volume55
Issue number6
Publication statusPublished - Dec 2001

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Caspase 9
Cartilage
Chondrocytes
Caspase 3
Apoptosis
Osteoarthritis
Cells
Arthroplasty
In Situ Nick-End Labeling
Caspases
Surgery
Labeling
Population
3-nitrotyrosine
Femoral Neck Fractures
Transmission electron microscopy
DNA Fragmentation
Thigh
DNA
Transmission Electron Microscopy

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Expression of caspase-3 and -9 relevant to cartilage destruction and chondrocyte apoptosis in human osteoarthritic cartilage. / Matsuo, M.; Nishida, Keiichiro; Yoshida, A.; Murakami, T.; Inoue, H.

In: Acta Medica Okayama, Vol. 55, No. 6, 12.2001, p. 333-340.

Research output: Contribution to journalArticle

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