Expression of c-fos gene inhibits proteoglycan synthesis in transfected chondrocyte

Michiko Tsuji, Shin Ichi Funahashi, Masaharu Takigawa, Motoharu Seiki, Katsuyuki Fujii, Takeshi Yoshida

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when compared to a control transfectant. A significant increase in transcription of MMP-3 with the suppressed transcription of aggrecan and TIMP-1 were also observed in the c-fos transfectants. Moreover, analysis of the effect of AP-1 proteins on the collagenase and TIMP-1 promoters in gastric carcinoma KKLS cells revealed that c-Fos combined with any of the Jun-related proteins failed to stimulate the TIMP-1 promoter, though collagenase promoter was effectively activated by any Fos/Jun-related protein heterocomplex. These findings indicate that the c-fos expression may govern the cartilage metabolism and hence may play an important role in the pathogenesis of joint destruction in arthritis.

Original languageEnglish
Pages (from-to)222-226
Number of pages5
JournalFEBS Letters
Volume381
Issue number3
DOIs
Publication statusPublished - Mar 4 1996

Fingerprint

fos Genes
Tissue Inhibitor of Metalloproteinase-1
Proteoglycans
Chondrocytes
Genes
Cartilage
Transcription
Metabolism
Aggrecans
Proteins
Transcription Factor AP-1
Collagenases
Matrix Metalloproteinases
Sulfates
Arthritis
Stomach
Joints
Carcinoma
Messenger RNA
DNA

Keywords

  • Activating protein-1
  • c-fos
  • Chondrocyte
  • Matrix metalloproteinase-3
  • Proteoglycan
  • Tissue inhibitor of metalloproteinase-1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Expression of c-fos gene inhibits proteoglycan synthesis in transfected chondrocyte. / Tsuji, Michiko; Funahashi, Shin Ichi; Takigawa, Masaharu; Seiki, Motoharu; Fujii, Katsuyuki; Yoshida, Takeshi.

In: FEBS Letters, Vol. 381, No. 3, 04.03.1996, p. 222-226.

Research output: Contribution to journalArticle

Tsuji, Michiko ; Funahashi, Shin Ichi ; Takigawa, Masaharu ; Seiki, Motoharu ; Fujii, Katsuyuki ; Yoshida, Takeshi. / Expression of c-fos gene inhibits proteoglycan synthesis in transfected chondrocyte. In: FEBS Letters. 1996 ; Vol. 381, No. 3. pp. 222-226.
@article{6c197a12aa6c482ca41acfa2703f0595,
title = "Expression of c-fos gene inhibits proteoglycan synthesis in transfected chondrocyte",
abstract = "The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when compared to a control transfectant. A significant increase in transcription of MMP-3 with the suppressed transcription of aggrecan and TIMP-1 were also observed in the c-fos transfectants. Moreover, analysis of the effect of AP-1 proteins on the collagenase and TIMP-1 promoters in gastric carcinoma KKLS cells revealed that c-Fos combined with any of the Jun-related proteins failed to stimulate the TIMP-1 promoter, though collagenase promoter was effectively activated by any Fos/Jun-related protein heterocomplex. These findings indicate that the c-fos expression may govern the cartilage metabolism and hence may play an important role in the pathogenesis of joint destruction in arthritis.",
keywords = "Activating protein-1, c-fos, Chondrocyte, Matrix metalloproteinase-3, Proteoglycan, Tissue inhibitor of metalloproteinase-1",
author = "Michiko Tsuji and Funahashi, {Shin Ichi} and Masaharu Takigawa and Motoharu Seiki and Katsuyuki Fujii and Takeshi Yoshida",
year = "1996",
month = "3",
day = "4",
doi = "10.1016/0014-5793(96)00118-4",
language = "English",
volume = "381",
pages = "222--226",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Expression of c-fos gene inhibits proteoglycan synthesis in transfected chondrocyte

AU - Tsuji, Michiko

AU - Funahashi, Shin Ichi

AU - Takigawa, Masaharu

AU - Seiki, Motoharu

AU - Fujii, Katsuyuki

AU - Yoshida, Takeshi

PY - 1996/3/4

Y1 - 1996/3/4

N2 - The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when compared to a control transfectant. A significant increase in transcription of MMP-3 with the suppressed transcription of aggrecan and TIMP-1 were also observed in the c-fos transfectants. Moreover, analysis of the effect of AP-1 proteins on the collagenase and TIMP-1 promoters in gastric carcinoma KKLS cells revealed that c-Fos combined with any of the Jun-related proteins failed to stimulate the TIMP-1 promoter, though collagenase promoter was effectively activated by any Fos/Jun-related protein heterocomplex. These findings indicate that the c-fos expression may govern the cartilage metabolism and hence may play an important role in the pathogenesis of joint destruction in arthritis.

AB - The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when compared to a control transfectant. A significant increase in transcription of MMP-3 with the suppressed transcription of aggrecan and TIMP-1 were also observed in the c-fos transfectants. Moreover, analysis of the effect of AP-1 proteins on the collagenase and TIMP-1 promoters in gastric carcinoma KKLS cells revealed that c-Fos combined with any of the Jun-related proteins failed to stimulate the TIMP-1 promoter, though collagenase promoter was effectively activated by any Fos/Jun-related protein heterocomplex. These findings indicate that the c-fos expression may govern the cartilage metabolism and hence may play an important role in the pathogenesis of joint destruction in arthritis.

KW - Activating protein-1

KW - c-fos

KW - Chondrocyte

KW - Matrix metalloproteinase-3

KW - Proteoglycan

KW - Tissue inhibitor of metalloproteinase-1

UR - http://www.scopus.com/inward/record.url?scp=0029925577&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029925577&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(96)00118-4

DO - 10.1016/0014-5793(96)00118-4

M3 - Article

C2 - 8601460

AN - SCOPUS:0029925577

VL - 381

SP - 222

EP - 226

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 3

ER -