Expression of c-fos gene inhibits proteoglycan synthesis in transfected chondrocyte

Michiko Tsuji, Shin Ichi Funahashi, Masaharu Takigawa, Motoharu Seiki, Katsuyuki Fujii, Takeshi Yoshida

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when compared to a control transfectant. A significant increase in transcription of MMP-3 with the suppressed transcription of aggrecan and TIMP-1 were also observed in the c-fos transfectants. Moreover, analysis of the effect of AP-1 proteins on the collagenase and TIMP-1 promoters in gastric carcinoma KKLS cells revealed that c-Fos combined with any of the Jun-related proteins failed to stimulate the TIMP-1 promoter, though collagenase promoter was effectively activated by any Fos/Jun-related protein heterocomplex. These findings indicate that the c-fos expression may govern the cartilage metabolism and hence may play an important role in the pathogenesis of joint destruction in arthritis.

Original languageEnglish
Pages (from-to)222-226
Number of pages5
JournalFEBS Letters
Volume381
Issue number3
DOIs
Publication statusPublished - Mar 4 1996

Keywords

  • Activating protein-1
  • Chondrocyte
  • Matrix metalloproteinase-3
  • Proteoglycan
  • Tissue inhibitor of metalloproteinase-1
  • c-fos

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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