Expression of amphiphysin I in Sertoli cells and its implication in spermatogenesis

Masami Watanabe, Kimiko Tsutsui, Osamu Hosoya, Ken Tsutsui, Hiromi Kumon, Akira Tokunaga

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Amphiphysin I is a protein concentrated in nerve terminals and involved in the endocytosis of synaptic vesicle membrane. We show here that amphiphysin I is expressed in the rat testis, localized exclusively in the Sertoli cells. In the postnatal testicular development, expression of amphiphysin I was not evident at birth, but became significant at postnatal day 15 (P15), coinciding with the onset of spermatogenesis. The expression level of amphiphysin I increased 10-fold between P15 and P25 to reach the adult level. In adult testes reversibly damaged by ethane dimethane sulphonate administration, expression of amphiphysin I did not change following the damage, whereas the protein was transiently converted into its phosphorylated form. The increase in levels of phosphorylated amphiphysin I was closely associated with the severe histological damage to germ cells. The present findings suggest that amphiphysin I in Sertoli cells is involved in spermatogenesis, probably through endocytic processes.

Original languageEnglish
Pages (from-to)739-745
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume287
Issue number3
DOIs
Publication statusPublished - Sep 28 2001

Keywords

  • Amphiphysin I
  • Ethane dimethane sulphonate
  • Phosphorylation
  • Sertoli cells
  • Spermatogenesis
  • Testis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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