Abstract
Amphiphysin (amph) I is a SH3 domain-containing 128kDa protein, concentrated in nerve terminals. The protein plays an essential role in clathrin-mediated endocytosis through an interaction with GTPase dynamin. Western blot analyses using anti-amph I specific antibody and anti-dynamin monoclonal antibody clearly detected their specific expressions in the testicular tissues from patients with testicular cancer(N=2), cryptorchidism (N=2), or azoospermia (N=3). At the same time, we investigated relationship between the expression levels of these proteins and Johnsen's score, a biopsy score indicating spermatogenesis in the human testis. A significant positive correlation between amph I expression and Johnsen's score was observed, but not between dynamin expression and Johnsen's score. The appropriate expression of amph I appears to play an important role in spermatogenesis by controlling the environment milieu within the seminiferous tubules through clathrin-mediated endocytosis.
| Original language | English |
|---|---|
| Pages (from-to) | 33-39 |
| Number of pages | 7 |
| Journal | Japanese Journal of Fertility and Sterility |
| Volume | 45 |
| Issue number | 2 |
| Publication status | Published - Jan 1 2000 |
Keywords
- Amphiphysin
- Dynamin
- Endocytosis
- Male infertility
- Spermatogenesis
ASJC Scopus subject areas
- Obstetrics and Gynaecology