Abstract
Amphiphysin (amph) I is a SH3 domain-containing 128kDa protein, concentrated in nerve terminals. The protein plays an essential role in clathrin-mediated endocytosis through an interaction with GTPase dynamin. Western blot analyses using anti-amph I specific antibody and anti-dynamin monoclonal antibody clearly detected their specific expressions in the testicular tissues from patients with testicular cancer(N=2), cryptorchidism (N=2), or azoospermia (N=3). At the same time, we investigated relationship between the expression levels of these proteins and Johnsen's score, a biopsy score indicating spermatogenesis in the human testis. A significant positive correlation between amph I expression and Johnsen's score was observed, but not between dynamin expression and Johnsen's score. The appropriate expression of amph I appears to play an important role in spermatogenesis by controlling the environment milieu within the seminiferous tubules through clathrin-mediated endocytosis.
| Original language | English |
|---|---|
| Pages (from-to) | 33-39 |
| Number of pages | 7 |
| Journal | Japanese Journal of Fertility and Sterility |
| Volume | 45 |
| Issue number | 2 |
| Publication status | Published - 2000 |
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Keywords
- Amphiphysin
- Dynamin
- Endocytosis
- Male infertility
- Spermatogenesis
ASJC Scopus subject areas
- Obstetrics and Gynaecology
Cite this
Expression of amphiphysin I, a nerve terminal-enriched protein, in the human testis. / Watanabe, Masami.
In: Japanese Journal of Fertility and Sterility, Vol. 45, No. 2, 2000, p. 33-39.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of amphiphysin I, a nerve terminal-enriched protein, in the human testis
AU - Watanabe, Masami
PY - 2000
Y1 - 2000
N2 - Amphiphysin (amph) I is a SH3 domain-containing 128kDa protein, concentrated in nerve terminals. The protein plays an essential role in clathrin-mediated endocytosis through an interaction with GTPase dynamin. Western blot analyses using anti-amph I specific antibody and anti-dynamin monoclonal antibody clearly detected their specific expressions in the testicular tissues from patients with testicular cancer(N=2), cryptorchidism (N=2), or azoospermia (N=3). At the same time, we investigated relationship between the expression levels of these proteins and Johnsen's score, a biopsy score indicating spermatogenesis in the human testis. A significant positive correlation between amph I expression and Johnsen's score was observed, but not between dynamin expression and Johnsen's score. The appropriate expression of amph I appears to play an important role in spermatogenesis by controlling the environment milieu within the seminiferous tubules through clathrin-mediated endocytosis.
AB - Amphiphysin (amph) I is a SH3 domain-containing 128kDa protein, concentrated in nerve terminals. The protein plays an essential role in clathrin-mediated endocytosis through an interaction with GTPase dynamin. Western blot analyses using anti-amph I specific antibody and anti-dynamin monoclonal antibody clearly detected their specific expressions in the testicular tissues from patients with testicular cancer(N=2), cryptorchidism (N=2), or azoospermia (N=3). At the same time, we investigated relationship between the expression levels of these proteins and Johnsen's score, a biopsy score indicating spermatogenesis in the human testis. A significant positive correlation between amph I expression and Johnsen's score was observed, but not between dynamin expression and Johnsen's score. The appropriate expression of amph I appears to play an important role in spermatogenesis by controlling the environment milieu within the seminiferous tubules through clathrin-mediated endocytosis.
KW - Amphiphysin
KW - Dynamin
KW - Endocytosis
KW - Male infertility
KW - Spermatogenesis
UR - http://www.scopus.com/inward/record.url?scp=0034098579&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034098579&partnerID=8YFLogxK
M3 - Article
VL - 45
SP - 33
EP - 39
JO - Japanese Journal of Fertility and Sterility
JF - Japanese Journal of Fertility and Sterility
SN - 0029-0629
IS - 2
ER -