Abstract
Amphiphysin (amph) I is a SH3 domain-containing 128kDa protein, concentrated in nerve terminals. The protein plays an essential role in clathrin-mediated endocytosis through an interaction with GTPase dynamin. Western blot analyses using anti-amph I specific antibody and anti-dynamin monoclonal antibody clearly detected their specific expressions in the testicular tissues from patients with testicular cancer(N=2), cryptorchidism (N=2), or azoospermia (N=3). At the same time, we investigated relationship between the expression levels of these proteins and Johnsen's score, a biopsy score indicating spermatogenesis in the human testis. A significant positive correlation between amph I expression and Johnsen's score was observed, but not between dynamin expression and Johnsen's score. The appropriate expression of amph I appears to play an important role in spermatogenesis by controlling the environment milieu within the seminiferous tubules through clathrin-mediated endocytosis.
Original language | English |
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Pages (from-to) | 33-39 |
Number of pages | 7 |
Journal | Japanese Journal of Fertility and Sterility |
Volume | 45 |
Issue number | 2 |
Publication status | Published - Jan 1 2000 |
Keywords
- Amphiphysin
- Dynamin
- Endocytosis
- Male infertility
- Spermatogenesis
ASJC Scopus subject areas
- Obstetrics and Gynaecology