Expression of amphiphysin I, a nerve terminal-enriched protein, in the human testis

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Abstract

Amphiphysin (amph) I is a SH3 domain-containing 128kDa protein, concentrated in nerve terminals. The protein plays an essential role in clathrin-mediated endocytosis through an interaction with GTPase dynamin. Western blot analyses using anti-amph I specific antibody and anti-dynamin monoclonal antibody clearly detected their specific expressions in the testicular tissues from patients with testicular cancer(N=2), cryptorchidism (N=2), or azoospermia (N=3). At the same time, we investigated relationship between the expression levels of these proteins and Johnsen's score, a biopsy score indicating spermatogenesis in the human testis. A significant positive correlation between amph I expression and Johnsen's score was observed, but not between dynamin expression and Johnsen's score. The appropriate expression of amph I appears to play an important role in spermatogenesis by controlling the environment milieu within the seminiferous tubules through clathrin-mediated endocytosis.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalJapanese Journal of Fertility and Sterility
Volume45
Issue number2
Publication statusPublished - Jan 1 2000

Keywords

  • Amphiphysin
  • Dynamin
  • Endocytosis
  • Male infertility
  • Spermatogenesis

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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