Amphiphysin (amph) I is a SH3 domain-containing 128kDa protein, concentrated in nerve terminals. The protein plays an essential role in clathrin-mediated endocytosis through an interaction with GTPase dynamin. Western blot analyses using anti-amph I specific antibody and anti-dynamin monoclonal antibody clearly detected their specific expressions in the testicular tissues from patients with testicular cancer(N=2), cryptorchidism (N=2), or azoospermia (N=3). At the same time, we investigated relationship between the expression levels of these proteins and Johnsen's score, a biopsy score indicating spermatogenesis in the human testis. A significant positive correlation between amph I expression and Johnsen's score was observed, but not between dynamin expression and Johnsen's score. The appropriate expression of amph I appears to play an important role in spermatogenesis by controlling the environment milieu within the seminiferous tubules through clathrin-mediated endocytosis.
|Number of pages||7|
|Journal||Japanese Journal of Fertility and Sterility|
|Publication status||Published - 2000|
- Male infertility
ASJC Scopus subject areas
- Obstetrics and Gynaecology