Expression of aluminum-induced genes in transgenic Arabidopsis plants can ameliorate aluminum stress and/or oxidative stress

B. Ezaki, R. C. Gardner, Y. Ezaki, H. Matsumoto

Research output: Contribution to journalArticle

296 Citations (Scopus)

Abstract

To examine the biological role of Al-stress-induced genes, nine genes derived from Arabidopsis, tobacco (Nicotiana tabacum L.), wheat (Triticum aestivum L.), and yeast (Saccharomyces cerevisiae) were expressed in Arabidopsis ecotype Landsberg. Lines containing eight of these genes were phenotypically normal and were tested in root elongation assays for their sensitivity to Al, Cd, Cu, Na, Zn, and to oxidative stresses. An Arabidopsis blue-copper-binding protein gene (AtBCB), a tobacco glutathione S-transferase gene (parB), a tobacco peroxidase gene (NtPox), and a tobacco GDP-dissociation inhibitor gene (NtGDI1) conferred a degree of resistance to Al. Two of these genes, AtBCB and parB, and a peroxidase gene from Arabidopsis (AtPox) also showed increased resistance to oxidative stress induced by diamide, while parB conferred resistance to Cu and Na. Al content of Al-treated root tips was reduced in the four Al-resistant plant lines compared with wild-type Ler-0, as judged by morin staining. All four Al-resistant lines also showed reduced staining of roots with 2',7'-dichloro fluorescein diacetate (H2DCFDA), an indicator of oxidative stress. We conclude that Al-induced genes can serve to protect against Al toxicity, and also provide genetic evidence for a link between Al stress and oxidative stress in plants.

Original languageEnglish
Pages (from-to)657-665
Number of pages9
JournalPlant Physiology
Volume122
Issue number3
Publication statusPublished - 2000

Fingerprint

Genetically Modified Plants
Aluminum
Arabidopsis
aluminum
Oxidative Stress
oxidative stress
genetically modified organisms
Genes
Tobacco
genes
tobacco
Peroxidase
Triticum
peroxidase
Guanine Nucleotide Dissociation Inhibitors
Staining and Labeling
Diamide
Ecotype
Meristem
fluorescein

ASJC Scopus subject areas

  • Plant Science

Cite this

Expression of aluminum-induced genes in transgenic Arabidopsis plants can ameliorate aluminum stress and/or oxidative stress. / Ezaki, B.; Gardner, R. C.; Ezaki, Y.; Matsumoto, H.

In: Plant Physiology, Vol. 122, No. 3, 2000, p. 657-665.

Research output: Contribution to journalArticle

Ezaki, B. ; Gardner, R. C. ; Ezaki, Y. ; Matsumoto, H. / Expression of aluminum-induced genes in transgenic Arabidopsis plants can ameliorate aluminum stress and/or oxidative stress. In: Plant Physiology. 2000 ; Vol. 122, No. 3. pp. 657-665.
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