Expression of adenovirus-mediated E. coli lacZ gene in skeletal muscles and spinal motor neurons of transgenic mice with a mutant superoxide dismutase gene

Hitoshi Warita, Koji Abe, Yasuhiro Setoguchi, Yasuto Itoyama

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A replication-defective recombinant adenoviral vector containing E. coli lacZ gene was injected into the right biceps brachii muscles of transgenic mice carrying mutant human Cu/Zn superoxide dismutase (SOD1) gene and non- transgenic wild-type mice at 27 weeks of age. Although the transgenic mice showed remarkable neurogenic muscular changes and a marked motor neuron loss in the anterior horn of spinal cord, the lacZ gene was widely expressed in all the injected muscles of transgenic mice as well as of wild-type mice at 7 days after the injection. In one transgenic and two wild-type mice, the lacZ gene expression was first detected in a few motor neurons of right lower cervical cord (C5-C6). These results demonstrate that an adenovirus-mediated foreign gene is transferred and expressed in skeletal muscles both of normal and transgenic mice model for familial amyotrophic lateral sclerosis (FALS), and also, in the spinal motor neurons, may be transferred by retrograde transport from innervated muscles.

Original languageEnglish
Pages (from-to)153-156
Number of pages4
JournalNeuroscience Letters
Volume246
Issue number3
DOIs
Publication statusPublished - Apr 10 1998
Externally publishedYes

Fingerprint

Lac Operon
Motor Neurons
Adenoviridae
Transgenic Mice
Superoxide Dismutase
Skeletal Muscle
Escherichia coli
Genes
Muscles
Gene Expression
Injections

Keywords

  • Familial amyotrophic lateral sclerosis
  • Gene transfer
  • lacZ gene
  • Superoxide dismutase
  • Transgenic mouse

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of adenovirus-mediated E. coli lacZ gene in skeletal muscles and spinal motor neurons of transgenic mice with a mutant superoxide dismutase gene. / Warita, Hitoshi; Abe, Koji; Setoguchi, Yasuhiro; Itoyama, Yasuto.

In: Neuroscience Letters, Vol. 246, No. 3, 10.04.1998, p. 153-156.

Research output: Contribution to journalArticle

@article{c4f6ff6e431d473e81ca7b0ea16c44a3,
title = "Expression of adenovirus-mediated E. coli lacZ gene in skeletal muscles and spinal motor neurons of transgenic mice with a mutant superoxide dismutase gene",
abstract = "A replication-defective recombinant adenoviral vector containing E. coli lacZ gene was injected into the right biceps brachii muscles of transgenic mice carrying mutant human Cu/Zn superoxide dismutase (SOD1) gene and non- transgenic wild-type mice at 27 weeks of age. Although the transgenic mice showed remarkable neurogenic muscular changes and a marked motor neuron loss in the anterior horn of spinal cord, the lacZ gene was widely expressed in all the injected muscles of transgenic mice as well as of wild-type mice at 7 days after the injection. In one transgenic and two wild-type mice, the lacZ gene expression was first detected in a few motor neurons of right lower cervical cord (C5-C6). These results demonstrate that an adenovirus-mediated foreign gene is transferred and expressed in skeletal muscles both of normal and transgenic mice model for familial amyotrophic lateral sclerosis (FALS), and also, in the spinal motor neurons, may be transferred by retrograde transport from innervated muscles.",
keywords = "Familial amyotrophic lateral sclerosis, Gene transfer, lacZ gene, Superoxide dismutase, Transgenic mouse",
author = "Hitoshi Warita and Koji Abe and Yasuhiro Setoguchi and Yasuto Itoyama",
year = "1998",
month = "4",
day = "10",
doi = "10.1016/S0304-3940(98)00245-6",
language = "English",
volume = "246",
pages = "153--156",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Expression of adenovirus-mediated E. coli lacZ gene in skeletal muscles and spinal motor neurons of transgenic mice with a mutant superoxide dismutase gene

AU - Warita, Hitoshi

AU - Abe, Koji

AU - Setoguchi, Yasuhiro

AU - Itoyama, Yasuto

PY - 1998/4/10

Y1 - 1998/4/10

N2 - A replication-defective recombinant adenoviral vector containing E. coli lacZ gene was injected into the right biceps brachii muscles of transgenic mice carrying mutant human Cu/Zn superoxide dismutase (SOD1) gene and non- transgenic wild-type mice at 27 weeks of age. Although the transgenic mice showed remarkable neurogenic muscular changes and a marked motor neuron loss in the anterior horn of spinal cord, the lacZ gene was widely expressed in all the injected muscles of transgenic mice as well as of wild-type mice at 7 days after the injection. In one transgenic and two wild-type mice, the lacZ gene expression was first detected in a few motor neurons of right lower cervical cord (C5-C6). These results demonstrate that an adenovirus-mediated foreign gene is transferred and expressed in skeletal muscles both of normal and transgenic mice model for familial amyotrophic lateral sclerosis (FALS), and also, in the spinal motor neurons, may be transferred by retrograde transport from innervated muscles.

AB - A replication-defective recombinant adenoviral vector containing E. coli lacZ gene was injected into the right biceps brachii muscles of transgenic mice carrying mutant human Cu/Zn superoxide dismutase (SOD1) gene and non- transgenic wild-type mice at 27 weeks of age. Although the transgenic mice showed remarkable neurogenic muscular changes and a marked motor neuron loss in the anterior horn of spinal cord, the lacZ gene was widely expressed in all the injected muscles of transgenic mice as well as of wild-type mice at 7 days after the injection. In one transgenic and two wild-type mice, the lacZ gene expression was first detected in a few motor neurons of right lower cervical cord (C5-C6). These results demonstrate that an adenovirus-mediated foreign gene is transferred and expressed in skeletal muscles both of normal and transgenic mice model for familial amyotrophic lateral sclerosis (FALS), and also, in the spinal motor neurons, may be transferred by retrograde transport from innervated muscles.

KW - Familial amyotrophic lateral sclerosis

KW - Gene transfer

KW - lacZ gene

KW - Superoxide dismutase

KW - Transgenic mouse

UR - http://www.scopus.com/inward/record.url?scp=0032502573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032502573&partnerID=8YFLogxK

U2 - 10.1016/S0304-3940(98)00245-6

DO - 10.1016/S0304-3940(98)00245-6

M3 - Article

VL - 246

SP - 153

EP - 156

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -