Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells

Possible involvement of peroxidase isozymes in aluminum ion stress

Bunichi Ezaki, Shinobu Tsugita, Hideaki Matsumoto

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

To clarify the mechanism of aluminum (Al) toxicity and Al tolerance, we isolated a new clone (pAL201) from a tobacco cDNA library. Northern blot hybridization analysis indicated that the expression of pAL201 is induced by Al treatment and phosphate (Pi) starvation. The complete cDNA sequence suggested that this clone encodes a moderately anionic peroxidase (EC 1.11.1.7). Analysis by isoelectric focussing indicated that a moderately anionic peroxidase (approximately pI 6.7) and two cationic peroxidases (pI 9.2 and 9.7) in the soluble fraction are activated by Al treatment and Pi starvation, while two moderately anionic isozymes are repressed by these stresses. We suppose that Al ion stress can control the activity of some peroxidase isozymes, one of which is probably induced by enhanced gene expression of pAL201. There is a possibility that some of these isozymes have some functions in Al ion stress.

Original languageEnglish
Pages (from-to)21-28
Number of pages8
JournalPhysiologia Plantarum
Volume96
Issue number1
Publication statusPublished - Jan 1996

Fingerprint

Aluminum
Peroxidase
Isoenzymes
Tobacco
aluminum
isozymes
peroxidase
tobacco
Ions
ions
Starvation
cells
Clone Cells
starvation
Peroxidases
clones
Isoelectric Focusing
Gene Library
Northern Blotting
peroxidases

Keywords

  • Aluminum (Al) ion stress
  • Gene expression
  • Metal toxicity
  • Nicotiana tabacum cultured cells
  • Peroxidase
  • Phosphate (P) starvation

ASJC Scopus subject areas

  • Plant Science

Cite this

Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells : Possible involvement of peroxidase isozymes in aluminum ion stress. / Ezaki, Bunichi; Tsugita, Shinobu; Matsumoto, Hideaki.

In: Physiologia Plantarum, Vol. 96, No. 1, 01.1996, p. 21-28.

Research output: Contribution to journalArticle

@article{6ce53b3110884ff3ba18c820d31d6b68,
title = "Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells: Possible involvement of peroxidase isozymes in aluminum ion stress",
abstract = "To clarify the mechanism of aluminum (Al) toxicity and Al tolerance, we isolated a new clone (pAL201) from a tobacco cDNA library. Northern blot hybridization analysis indicated that the expression of pAL201 is induced by Al treatment and phosphate (Pi) starvation. The complete cDNA sequence suggested that this clone encodes a moderately anionic peroxidase (EC 1.11.1.7). Analysis by isoelectric focussing indicated that a moderately anionic peroxidase (approximately pI 6.7) and two cationic peroxidases (pI 9.2 and 9.7) in the soluble fraction are activated by Al treatment and Pi starvation, while two moderately anionic isozymes are repressed by these stresses. We suppose that Al ion stress can control the activity of some peroxidase isozymes, one of which is probably induced by enhanced gene expression of pAL201. There is a possibility that some of these isozymes have some functions in Al ion stress.",
keywords = "Aluminum (Al) ion stress, Gene expression, Metal toxicity, Nicotiana tabacum cultured cells, Peroxidase, Phosphate (P) starvation",
author = "Bunichi Ezaki and Shinobu Tsugita and Hideaki Matsumoto",
year = "1996",
month = "1",
language = "English",
volume = "96",
pages = "21--28",
journal = "Physiologia Plantarum",
issn = "0031-9317",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Expression of a moderately anionic peroxidase is induced by aluminum treatment in tobacco cells

T2 - Possible involvement of peroxidase isozymes in aluminum ion stress

AU - Ezaki, Bunichi

AU - Tsugita, Shinobu

AU - Matsumoto, Hideaki

PY - 1996/1

Y1 - 1996/1

N2 - To clarify the mechanism of aluminum (Al) toxicity and Al tolerance, we isolated a new clone (pAL201) from a tobacco cDNA library. Northern blot hybridization analysis indicated that the expression of pAL201 is induced by Al treatment and phosphate (Pi) starvation. The complete cDNA sequence suggested that this clone encodes a moderately anionic peroxidase (EC 1.11.1.7). Analysis by isoelectric focussing indicated that a moderately anionic peroxidase (approximately pI 6.7) and two cationic peroxidases (pI 9.2 and 9.7) in the soluble fraction are activated by Al treatment and Pi starvation, while two moderately anionic isozymes are repressed by these stresses. We suppose that Al ion stress can control the activity of some peroxidase isozymes, one of which is probably induced by enhanced gene expression of pAL201. There is a possibility that some of these isozymes have some functions in Al ion stress.

AB - To clarify the mechanism of aluminum (Al) toxicity and Al tolerance, we isolated a new clone (pAL201) from a tobacco cDNA library. Northern blot hybridization analysis indicated that the expression of pAL201 is induced by Al treatment and phosphate (Pi) starvation. The complete cDNA sequence suggested that this clone encodes a moderately anionic peroxidase (EC 1.11.1.7). Analysis by isoelectric focussing indicated that a moderately anionic peroxidase (approximately pI 6.7) and two cationic peroxidases (pI 9.2 and 9.7) in the soluble fraction are activated by Al treatment and Pi starvation, while two moderately anionic isozymes are repressed by these stresses. We suppose that Al ion stress can control the activity of some peroxidase isozymes, one of which is probably induced by enhanced gene expression of pAL201. There is a possibility that some of these isozymes have some functions in Al ion stress.

KW - Aluminum (Al) ion stress

KW - Gene expression

KW - Metal toxicity

KW - Nicotiana tabacum cultured cells

KW - Peroxidase

KW - Phosphate (P) starvation

UR - http://www.scopus.com/inward/record.url?scp=0030019256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030019256&partnerID=8YFLogxK

M3 - Article

VL - 96

SP - 21

EP - 28

JO - Physiologia Plantarum

JF - Physiologia Plantarum

SN - 0031-9317

IS - 1

ER -