Expression of a 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene in peach (Prunus persica L.) fruit in response to treatment with carbon dioxide and 1-methylcyclopropene

Possible role of ethylene

Francis M. Mathooko, Yuki Tsunashima, Yasutaka Kubo, Akitsugu Inaba

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In this study we investigated the effect of exogenous ethylene treatment on ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) oxidase activity and expression of an ACC oxidase (PP-ACO1) gene previously cloned (Mathooko et al., 2001) in peach (Prunus persica L.) fruit. We also investigated the mode of action of CO2 and 1-methycyclopropene (1-MCP) in the regulation of ethylene biosynthesis during peach fruit ripening. Fruits were treated with various concentrations of ethylene (0.1, 1, 10, 100, 500, 1000 ppm) and also with CO2 and 1-MCP in the presence or absence of 500 ppm ethylene. Ethylene stimulated ethylene production at concentrations of 100 ppm and above while ACC oxidase activity was stimulated in a concentration-dependent manner. PP-ACO1 was slightly constitutively expressed and exogenous ethylene stimulated accumulation of its mRNA transcript in a concentration-dependent manner up to 100 ppm after which the level remained constant. CO2 and 1-MCP inhibited the ethylenestimulated ethylene production, ACC oxidase activity and accumulation of PP-ACO1 transcripts by about 50%. These results indicate that ethylene plays a key role in the regulation of ethylene production and ACC oxidase activity and its gene expression in peach fruit. Further the results indicate that CO2 and 1-MCP regulate ethylene biosynthesis in peach fruit during ripening, at least in part, by antagonizing ethylene action.

Original languageEnglish
Pages (from-to)497-502
Number of pages6
JournalAfrican Journal of Biotechnology
Volume3
Issue number10
Publication statusPublished - Oct 2004

Fingerprint

Prunus persica
1-methylcyclopropene
Carbon Dioxide
peaches
ethylene
Fruit
ethylene production
carbon dioxide
fruits
Genes
genes
ripening
1-aminocyclopropane-1-carboxylic acid oxidase
mechanism of action
gene expression

Keywords

  • 1-methylcyclopropene
  • ACC oxidase
  • Carbon dioxide
  • Ehylene
  • Peach
  • Prunus persica

ASJC Scopus subject areas

  • Biotechnology

Cite this

@article{953b5396c9124949afbbbca5207576ce,
title = "Expression of a 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene in peach (Prunus persica L.) fruit in response to treatment with carbon dioxide and 1-methylcyclopropene: Possible role of ethylene",
abstract = "In this study we investigated the effect of exogenous ethylene treatment on ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) oxidase activity and expression of an ACC oxidase (PP-ACO1) gene previously cloned (Mathooko et al., 2001) in peach (Prunus persica L.) fruit. We also investigated the mode of action of CO2 and 1-methycyclopropene (1-MCP) in the regulation of ethylene biosynthesis during peach fruit ripening. Fruits were treated with various concentrations of ethylene (0.1, 1, 10, 100, 500, 1000 ppm) and also with CO2 and 1-MCP in the presence or absence of 500 ppm ethylene. Ethylene stimulated ethylene production at concentrations of 100 ppm and above while ACC oxidase activity was stimulated in a concentration-dependent manner. PP-ACO1 was slightly constitutively expressed and exogenous ethylene stimulated accumulation of its mRNA transcript in a concentration-dependent manner up to 100 ppm after which the level remained constant. CO2 and 1-MCP inhibited the ethylenestimulated ethylene production, ACC oxidase activity and accumulation of PP-ACO1 transcripts by about 50{\%}. These results indicate that ethylene plays a key role in the regulation of ethylene production and ACC oxidase activity and its gene expression in peach fruit. Further the results indicate that CO2 and 1-MCP regulate ethylene biosynthesis in peach fruit during ripening, at least in part, by antagonizing ethylene action.",
keywords = "1-methylcyclopropene, ACC oxidase, Carbon dioxide, Ehylene, Peach, Prunus persica",
author = "Mathooko, {Francis M.} and Yuki Tsunashima and Yasutaka Kubo and Akitsugu Inaba",
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T1 - Expression of a 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene in peach (Prunus persica L.) fruit in response to treatment with carbon dioxide and 1-methylcyclopropene

T2 - Possible role of ethylene

AU - Mathooko, Francis M.

AU - Tsunashima, Yuki

AU - Kubo, Yasutaka

AU - Inaba, Akitsugu

PY - 2004/10

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N2 - In this study we investigated the effect of exogenous ethylene treatment on ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) oxidase activity and expression of an ACC oxidase (PP-ACO1) gene previously cloned (Mathooko et al., 2001) in peach (Prunus persica L.) fruit. We also investigated the mode of action of CO2 and 1-methycyclopropene (1-MCP) in the regulation of ethylene biosynthesis during peach fruit ripening. Fruits were treated with various concentrations of ethylene (0.1, 1, 10, 100, 500, 1000 ppm) and also with CO2 and 1-MCP in the presence or absence of 500 ppm ethylene. Ethylene stimulated ethylene production at concentrations of 100 ppm and above while ACC oxidase activity was stimulated in a concentration-dependent manner. PP-ACO1 was slightly constitutively expressed and exogenous ethylene stimulated accumulation of its mRNA transcript in a concentration-dependent manner up to 100 ppm after which the level remained constant. CO2 and 1-MCP inhibited the ethylenestimulated ethylene production, ACC oxidase activity and accumulation of PP-ACO1 transcripts by about 50%. These results indicate that ethylene plays a key role in the regulation of ethylene production and ACC oxidase activity and its gene expression in peach fruit. Further the results indicate that CO2 and 1-MCP regulate ethylene biosynthesis in peach fruit during ripening, at least in part, by antagonizing ethylene action.

AB - In this study we investigated the effect of exogenous ethylene treatment on ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) oxidase activity and expression of an ACC oxidase (PP-ACO1) gene previously cloned (Mathooko et al., 2001) in peach (Prunus persica L.) fruit. We also investigated the mode of action of CO2 and 1-methycyclopropene (1-MCP) in the regulation of ethylene biosynthesis during peach fruit ripening. Fruits were treated with various concentrations of ethylene (0.1, 1, 10, 100, 500, 1000 ppm) and also with CO2 and 1-MCP in the presence or absence of 500 ppm ethylene. Ethylene stimulated ethylene production at concentrations of 100 ppm and above while ACC oxidase activity was stimulated in a concentration-dependent manner. PP-ACO1 was slightly constitutively expressed and exogenous ethylene stimulated accumulation of its mRNA transcript in a concentration-dependent manner up to 100 ppm after which the level remained constant. CO2 and 1-MCP inhibited the ethylenestimulated ethylene production, ACC oxidase activity and accumulation of PP-ACO1 transcripts by about 50%. These results indicate that ethylene plays a key role in the regulation of ethylene production and ACC oxidase activity and its gene expression in peach fruit. Further the results indicate that CO2 and 1-MCP regulate ethylene biosynthesis in peach fruit during ripening, at least in part, by antagonizing ethylene action.

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