Abstract
In this study we investigated the effect of exogenous ethylene treatment on ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) oxidase activity and expression of an ACC oxidase (PP-ACO1) gene previously cloned (Mathooko et al., 2001) in peach (Prunus persica L.) fruit. We also investigated the mode of action of CO2 and 1-methycyclopropene (1-MCP) in the regulation of ethylene biosynthesis during peach fruit ripening. Fruits were treated with various concentrations of ethylene (0.1, 1, 10, 100, 500, 1000 ppm) and also with CO2 and 1-MCP in the presence or absence of 500 ppm ethylene. Ethylene stimulated ethylene production at concentrations of 100 ppm and above while ACC oxidase activity was stimulated in a concentration-dependent manner. PP-ACO1 was slightly constitutively expressed and exogenous ethylene stimulated accumulation of its mRNA transcript in a concentration-dependent manner up to 100 ppm after which the level remained constant. CO2 and 1-MCP inhibited the ethylenestimulated ethylene production, ACC oxidase activity and accumulation of PP-ACO1 transcripts by about 50%. These results indicate that ethylene plays a key role in the regulation of ethylene production and ACC oxidase activity and its gene expression in peach fruit. Further the results indicate that CO2 and 1-MCP regulate ethylene biosynthesis in peach fruit during ripening, at least in part, by antagonizing ethylene action.
| Original language | English |
|---|---|
| Pages (from-to) | 497-502 |
| Number of pages | 6 |
| Journal | African Journal of Biotechnology |
| Volume | 3 |
| Issue number | 10 |
| Publication status | Published - Oct 2004 |
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Keywords
- 1-methylcyclopropene
- ACC oxidase
- Carbon dioxide
- Ehylene
- Peach
- Prunus persica
ASJC Scopus subject areas
- Biotechnology
Cite this
Expression of a 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene in peach (Prunus persica L.) fruit in response to treatment with carbon dioxide and 1-methylcyclopropene : Possible role of ethylene. / Mathooko, Francis M.; Tsunashima, Yuki; Kubo, Yasutaka; Inaba, Akitsugu.
In: African Journal of Biotechnology, Vol. 3, No. 10, 10.2004, p. 497-502.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression of a 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene in peach (Prunus persica L.) fruit in response to treatment with carbon dioxide and 1-methylcyclopropene
T2 - Possible role of ethylene
AU - Mathooko, Francis M.
AU - Tsunashima, Yuki
AU - Kubo, Yasutaka
AU - Inaba, Akitsugu
PY - 2004/10
Y1 - 2004/10
N2 - In this study we investigated the effect of exogenous ethylene treatment on ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) oxidase activity and expression of an ACC oxidase (PP-ACO1) gene previously cloned (Mathooko et al., 2001) in peach (Prunus persica L.) fruit. We also investigated the mode of action of CO2 and 1-methycyclopropene (1-MCP) in the regulation of ethylene biosynthesis during peach fruit ripening. Fruits were treated with various concentrations of ethylene (0.1, 1, 10, 100, 500, 1000 ppm) and also with CO2 and 1-MCP in the presence or absence of 500 ppm ethylene. Ethylene stimulated ethylene production at concentrations of 100 ppm and above while ACC oxidase activity was stimulated in a concentration-dependent manner. PP-ACO1 was slightly constitutively expressed and exogenous ethylene stimulated accumulation of its mRNA transcript in a concentration-dependent manner up to 100 ppm after which the level remained constant. CO2 and 1-MCP inhibited the ethylenestimulated ethylene production, ACC oxidase activity and accumulation of PP-ACO1 transcripts by about 50%. These results indicate that ethylene plays a key role in the regulation of ethylene production and ACC oxidase activity and its gene expression in peach fruit. Further the results indicate that CO2 and 1-MCP regulate ethylene biosynthesis in peach fruit during ripening, at least in part, by antagonizing ethylene action.
AB - In this study we investigated the effect of exogenous ethylene treatment on ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) oxidase activity and expression of an ACC oxidase (PP-ACO1) gene previously cloned (Mathooko et al., 2001) in peach (Prunus persica L.) fruit. We also investigated the mode of action of CO2 and 1-methycyclopropene (1-MCP) in the regulation of ethylene biosynthesis during peach fruit ripening. Fruits were treated with various concentrations of ethylene (0.1, 1, 10, 100, 500, 1000 ppm) and also with CO2 and 1-MCP in the presence or absence of 500 ppm ethylene. Ethylene stimulated ethylene production at concentrations of 100 ppm and above while ACC oxidase activity was stimulated in a concentration-dependent manner. PP-ACO1 was slightly constitutively expressed and exogenous ethylene stimulated accumulation of its mRNA transcript in a concentration-dependent manner up to 100 ppm after which the level remained constant. CO2 and 1-MCP inhibited the ethylenestimulated ethylene production, ACC oxidase activity and accumulation of PP-ACO1 transcripts by about 50%. These results indicate that ethylene plays a key role in the regulation of ethylene production and ACC oxidase activity and its gene expression in peach fruit. Further the results indicate that CO2 and 1-MCP regulate ethylene biosynthesis in peach fruit during ripening, at least in part, by antagonizing ethylene action.
KW - 1-methylcyclopropene
KW - ACC oxidase
KW - Carbon dioxide
KW - Ehylene
KW - Peach
KW - Prunus persica
UR - http://www.scopus.com/inward/record.url?scp=15444372738&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=15444372738&partnerID=8YFLogxK
M3 - Article
VL - 3
SP - 497
EP - 502
JO - African Journal of Biotechnology
JF - African Journal of Biotechnology
SN - 1684-5315
IS - 10
ER -