Abstract
In this study we investigated the effect of exogenous ethylene treatment on ethylene production, 1-aminocyclopropane-1-carboxylate (ACC) oxidase activity and expression of an ACC oxidase (PP-ACO1) gene previously cloned (Mathooko et al., 2001) in peach (Prunus persica L.) fruit. We also investigated the mode of action of CO2 and 1-methycyclopropene (1-MCP) in the regulation of ethylene biosynthesis during peach fruit ripening. Fruits were treated with various concentrations of ethylene (0.1, 1, 10, 100, 500, 1000 ppm) and also with CO2 and 1-MCP in the presence or absence of 500 ppm ethylene. Ethylene stimulated ethylene production at concentrations of 100 ppm and above while ACC oxidase activity was stimulated in a concentration-dependent manner. PP-ACO1 was slightly constitutively expressed and exogenous ethylene stimulated accumulation of its mRNA transcript in a concentration-dependent manner up to 100 ppm after which the level remained constant. CO2 and 1-MCP inhibited the ethylenestimulated ethylene production, ACC oxidase activity and accumulation of PP-ACO1 transcripts by about 50%. These results indicate that ethylene plays a key role in the regulation of ethylene production and ACC oxidase activity and its gene expression in peach fruit. Further the results indicate that CO2 and 1-MCP regulate ethylene biosynthesis in peach fruit during ripening, at least in part, by antagonizing ethylene action.
Original language | English |
---|---|
Pages (from-to) | 497-502 |
Number of pages | 6 |
Journal | African Journal of Biotechnology |
Volume | 3 |
Issue number | 10 |
DOIs | |
Publication status | Published - Oct 2004 |
Keywords
- 1-methylcyclopropene
- ACC oxidase
- Carbon dioxide
- Ehylene
- Peach
- Prunus persica
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Molecular Biology
- Genetics
- Agronomy and Crop Science