Expression in yeast of a fusion gene composed of the promoter of a heat-shock gene from arabidopsis and a bacterial gene for β-glucuronidase

Taku Takahashi, Naoto Yabe, Yoshibumi Komeda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Production of a functional β-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.

Original languageEnglish
Pages (from-to)161-164
Number of pages4
JournalPlant and Cell Physiology
Volume34
Issue number1
Publication statusPublished - Jan 1993
Externally publishedYes

Fingerprint

Arabidopsis
Bacterial Genes
gene fusion
Gene Fusion
Glucuronidase
heat shock
Promoter
Yeast
yeast
heat stress
Shock
Fusion
Fusion reactions
Hot Temperature
Genes
Heat
Yeasts
promoter regions
yeasts
Gene

Keywords

  • β-Glucuronidase
  • Arabidopsis thaliana
  • CAMP
  • Heat shock
  • Saccharomyces cerevisiae
  • Schizosaccharomyces pombe

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Expression in yeast of a fusion gene composed of the promoter of a heat-shock gene from arabidopsis and a bacterial gene for β-glucuronidase. / Takahashi, Taku; Yabe, Naoto; Komeda, Yoshibumi.

In: Plant and Cell Physiology, Vol. 34, No. 1, 01.1993, p. 161-164.

Research output: Contribution to journalArticle

@article{9cc8a377367b46fcb0ef358cd7a6a8f3,
title = "Expression in yeast of a fusion gene composed of the promoter of a heat-shock gene from arabidopsis and a bacterial gene for β-glucuronidase",
abstract = "Production of a functional β-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.",
keywords = "β-Glucuronidase, Arabidopsis thaliana, CAMP, Heat shock, Saccharomyces cerevisiae, Schizosaccharomyces pombe",
author = "Taku Takahashi and Naoto Yabe and Yoshibumi Komeda",
year = "1993",
month = "1",
language = "English",
volume = "34",
pages = "161--164",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Expression in yeast of a fusion gene composed of the promoter of a heat-shock gene from arabidopsis and a bacterial gene for β-glucuronidase

AU - Takahashi, Taku

AU - Yabe, Naoto

AU - Komeda, Yoshibumi

PY - 1993/1

Y1 - 1993/1

N2 - Production of a functional β-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.

AB - Production of a functional β-glucuronidase (GUS) protein was induced by exposure of exponentially growing yeast cells to heat shock after transformation of the GUS gene under the control of the promoter of the heat-shock gene, HSP18.2, from Arabidopsis. Yeast cyr and bcy mutations appeared to have essentially no effect.

KW - β-Glucuronidase

KW - Arabidopsis thaliana

KW - CAMP

KW - Heat shock

KW - Saccharomyces cerevisiae

KW - Schizosaccharomyces pombe

UR - http://www.scopus.com/inward/record.url?scp=0027341712&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027341712&partnerID=8YFLogxK

M3 - Article

C2 - 8025819

AN - SCOPUS:0027341712

VL - 34

SP - 161

EP - 164

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 1

ER -