Expression, crystallization and preliminary crystallographic data analysis of filamin a repeats 14-16

Adeleke Halilu Aguda, Amos Malle Sakwe, Lars Rask, Robert Charles Robinson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Human filamin A is a 280 kDa protein involved in actin-filament cross-linking. It is structurally divided into an actin-binding headpiece (ABD) and a rod domain containing 24 immunoglobulin-like (Ig) repeats. A fragment of human filamin A (Ig repeats 14-16) was cloned and expressed in Escherichia coli and the purified protein was crystallized in 1.6 M ammonium sulfate, 2% PEG 1000 and 100 mM HEPES pH 7.5. The crystals diffracted to 1.95 Å and belong to space group P212121, with unit-cell parameters a = 50.63, b = 52.10, c = 98.46 Å, α = β = γ = 90°.

Original languageEnglish
Pages (from-to)291-293
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume63
Issue number4
DOIs
Publication statusPublished - Dec 1 2007
Externally publishedYes

    Fingerprint

Keywords

  • ABP-280
  • Calponin homology
  • Cytoskeleton
  • Filamin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics
  • Medicine(all)

Cite this