Expression characteristics of CS-ACS1, CS-ACS2 and CS-ACS3, three members of the 1-aminocyclopropane-1-carboxylate synthase gene family in cucumber (Cucumis sativus L.) fruit under carbon dioxide stress

Francis M. Mathooko, Mercy W. Mwaniki, Akira Nakatsuka, Shinjiro Shiomi, Yasutaka Kubo, Akitsugu Inaba, Reinosuke Nakamura

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Abstract

We investigated the expression pattern of three 1-aminocyclopropane-1-carboxylate (ACC) synthase genes, CS-ACS1, CS-ACS2 and CS-ACS3 in cucumber (Cucumis sativus L.) fruit under CO2 stress. CO2 stress-induced ethylene production paralleled the accumulation of only CSACS1 transcripts which disappeared upon withdrawal of CO2. Cycloheximide inhibited the CO2 stress-induced ethylene production but superinduced the accumulation of CS-ACS1 transcript. At higher concentrations, cycloheximide also induced the accumulation of CS-ACS2 and CS-ACS3 transcripts. In the presence of CO2 and cycloheximide, the accumulation of CS-ACS2 transcript occurred within 1 h, disappeared after 3 h and increased greatly upon withdrawal of CO2. Inhibitors of protein kinase and types 1 and 2A protein phosphatases which inhibited and stimulated, respectively, CO2 stress-induced ethylene production had little effect on the expression of these genes. The results presented here identify CS-ACS1 as the main ACC synthase gene responsible for the increased ethylene biosynthesis in cucumber fruit under CO2 stress and suggest that this gene is a primary response gene and its expression is under negative control since it is expressed by treatment with cycloheximide. The results further suggest that the regulation of CO2 stress-induced ethylene biosynthesis by reversible protein phosphorylation does not result from enhanced ACC synthase transcription.

Original languageEnglish
Pages (from-to)164-172
Number of pages9
JournalPlant and Cell Physiology
Volume40
Issue number2
DOIs
Publication statusPublished - Jan 1 1999

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Keywords

  • ACC synthase
  • CO stress
  • Cucumber
  • Cucumis sativus
  • Ethylene biosynthesis
  • Gene expression
  • Signal transduction

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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