Abstract
We have characterized transcripts for three potassium channel homologs in the AKT/KAT subfamily (Shaker type) from the common ice plant (Mesembryanthemum crystallinum), with a focus on their expression during salt stress (up to 500 mM NaCl). Mkt1 and 2, Arabidopsis AKT homologs, and Kmt1, a KAT homolog, are members of small gene families with two to three isoforms each. Mkt1 is root specific; Mkt2 is found in leaves, flowers, and seed capsules; and Kmt1 is expressed in leaves and seed capsules. Mkt1 is present in all cells of the root, and in leaves a highly conserved isoform is detected present in all cells with highest abundance in the vasculature. MKT1 for which antibodies were made is localized to the plasma membrane. Following salt stress, MKT1 (transcripts and protein) is drastically down-regulated, Mkt2 transcripts do not change significantly, and Kmt1 is strongly and transiently (maximum at 6 h) up-regulated in leaves and stems. The detection and stress-dependent behavior of abundant transcripts representing subfamilies of potassium channels provides information about tissue specificity and the complex regulation of genes encoding potassium uptake systems in a halophytic plant.
| Original language | English |
|---|---|
| Pages (from-to) | 604-614 |
| Number of pages | 11 |
| Journal | Plant Physiology |
| Volume | 125 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2001 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Plant Science
Cite this
Expression and stress-dependent induction of potassium channel transcripts in the common ice plant. / Su, H.; Golldack, D.; Katsuhara, Maki; Zhao, C.; Bohnert, H. J.
In: Plant Physiology, Vol. 125, No. 2, 2001, p. 604-614.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression and stress-dependent induction of potassium channel transcripts in the common ice plant
AU - Su, H.
AU - Golldack, D.
AU - Katsuhara, Maki
AU - Zhao, C.
AU - Bohnert, H. J.
PY - 2001
Y1 - 2001
N2 - We have characterized transcripts for three potassium channel homologs in the AKT/KAT subfamily (Shaker type) from the common ice plant (Mesembryanthemum crystallinum), with a focus on their expression during salt stress (up to 500 mM NaCl). Mkt1 and 2, Arabidopsis AKT homologs, and Kmt1, a KAT homolog, are members of small gene families with two to three isoforms each. Mkt1 is root specific; Mkt2 is found in leaves, flowers, and seed capsules; and Kmt1 is expressed in leaves and seed capsules. Mkt1 is present in all cells of the root, and in leaves a highly conserved isoform is detected present in all cells with highest abundance in the vasculature. MKT1 for which antibodies were made is localized to the plasma membrane. Following salt stress, MKT1 (transcripts and protein) is drastically down-regulated, Mkt2 transcripts do not change significantly, and Kmt1 is strongly and transiently (maximum at 6 h) up-regulated in leaves and stems. The detection and stress-dependent behavior of abundant transcripts representing subfamilies of potassium channels provides information about tissue specificity and the complex regulation of genes encoding potassium uptake systems in a halophytic plant.
AB - We have characterized transcripts for three potassium channel homologs in the AKT/KAT subfamily (Shaker type) from the common ice plant (Mesembryanthemum crystallinum), with a focus on their expression during salt stress (up to 500 mM NaCl). Mkt1 and 2, Arabidopsis AKT homologs, and Kmt1, a KAT homolog, are members of small gene families with two to three isoforms each. Mkt1 is root specific; Mkt2 is found in leaves, flowers, and seed capsules; and Kmt1 is expressed in leaves and seed capsules. Mkt1 is present in all cells of the root, and in leaves a highly conserved isoform is detected present in all cells with highest abundance in the vasculature. MKT1 for which antibodies were made is localized to the plasma membrane. Following salt stress, MKT1 (transcripts and protein) is drastically down-regulated, Mkt2 transcripts do not change significantly, and Kmt1 is strongly and transiently (maximum at 6 h) up-regulated in leaves and stems. The detection and stress-dependent behavior of abundant transcripts representing subfamilies of potassium channels provides information about tissue specificity and the complex regulation of genes encoding potassium uptake systems in a halophytic plant.
UR - http://www.scopus.com/inward/record.url?scp=0035117740&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035117740&partnerID=8YFLogxK
U2 - 10.1104/pp.125.2.604
DO - 10.1104/pp.125.2.604
M3 - Article
C2 - 11161018
AN - SCOPUS:0035117740
VL - 125
SP - 604
EP - 614
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 2
ER -