Expression and stress-dependent induction of potassium channel transcripts in the common ice plant

H. Su, D. Golldack, Maki Katsuhara, C. Zhao, H. J. Bohnert

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

We have characterized transcripts for three potassium channel homologs in the AKT/KAT subfamily (Shaker type) from the common ice plant (Mesembryanthemum crystallinum), with a focus on their expression during salt stress (up to 500 mM NaCl). Mkt1 and 2, Arabidopsis AKT homologs, and Kmt1, a KAT homolog, are members of small gene families with two to three isoforms each. Mkt1 is root specific; Mkt2 is found in leaves, flowers, and seed capsules; and Kmt1 is expressed in leaves and seed capsules. Mkt1 is present in all cells of the root, and in leaves a highly conserved isoform is detected present in all cells with highest abundance in the vasculature. MKT1 for which antibodies were made is localized to the plasma membrane. Following salt stress, MKT1 (transcripts and protein) is drastically down-regulated, Mkt2 transcripts do not change significantly, and Kmt1 is strongly and transiently (maximum at 6 h) up-regulated in leaves and stems. The detection and stress-dependent behavior of abundant transcripts representing subfamilies of potassium channels provides information about tissue specificity and the complex regulation of genes encoding potassium uptake systems in a halophytic plant.

Original languageEnglish
Pages (from-to)604-614
Number of pages11
JournalPlant Physiology
Volume125
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Mesembryanthemum
Mesembryanthemum crystallinum
Cotyledon
Potassium Channels
potassium channels
Capsules
Protein Isoforms
Salt-Tolerant Plants
Salts
Organ Specificity
Arabidopsis
Genes
salt stress
leaves
Potassium
Cell Membrane
Antibodies
seeds
Proteins
plasma membrane

ASJC Scopus subject areas

  • Plant Science

Cite this

Expression and stress-dependent induction of potassium channel transcripts in the common ice plant. / Su, H.; Golldack, D.; Katsuhara, Maki; Zhao, C.; Bohnert, H. J.

In: Plant Physiology, Vol. 125, No. 2, 2001, p. 604-614.

Research output: Contribution to journalArticle

Su, H. ; Golldack, D. ; Katsuhara, Maki ; Zhao, C. ; Bohnert, H. J. / Expression and stress-dependent induction of potassium channel transcripts in the common ice plant. In: Plant Physiology. 2001 ; Vol. 125, No. 2. pp. 604-614.
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