Abstract
Objective: To characterise a natural antisense transcript of connective tissue growth factor. Materials and Methods: RNA from several cell lines was analysed by RNase protection assay to detect antisense transcripts. To characterise the regulatory aspect of the transcribed area, chimeras were constructed in which the firefly luciferase gene was fused with the corresponding segment of ctgf/ccn2, and the gene expression was monitored. Results: A natural antisense transcript complementary to the 3′-untranslated region of ctgf/ccn2 mRNA in cultured human tumour cells was detected. The luciferase gene fused with the full-length antisense 3′-untranslated region showed strikingly low levels of luciferase expression compared with the control. Based on a series of deletion analyses, the major repressive element was located in the middle portion within the 3′-untranslated region, which corresponded to the antisense transcribed area. Conclusion: These results suggest that controlled expression of antisense RNAs against the 3′-untranslated region of ctgf/ccn2 mRNA may be involved in the determination of phenotypes in certain oral malignancies.
| Original language | English |
|---|---|
| Pages (from-to) | 172-179 |
| Number of pages | 8 |
| Journal | Asian Journal of Oral and Maxillofacial Surgery |
| Volume | 18 |
| Issue number | 3 |
| Publication status | Published - 2006 |
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Keywords
- 3′-Untranslated regions
- Antisense
- Connective tissue growth factor
- RNA
ASJC Scopus subject areas
- Surgery
Cite this
Expression and regulation of an antisense RNA transcript of the human connective tissue growth factor gene in human tumour cells. / Kondo, Seiji; Kubota, Satoshi; Kawaki, Harumi; Moritani, Norifumi; Kagawa, Toshimasa; Ueno, Takaaki; Sugahara, Toshio; Takigawa, Masaharu.
In: Asian Journal of Oral and Maxillofacial Surgery, Vol. 18, No. 3, 2006, p. 172-179.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression and regulation of an antisense RNA transcript of the human connective tissue growth factor gene in human tumour cells
AU - Kondo, Seiji
AU - Kubota, Satoshi
AU - Kawaki, Harumi
AU - Moritani, Norifumi
AU - Kagawa, Toshimasa
AU - Ueno, Takaaki
AU - Sugahara, Toshio
AU - Takigawa, Masaharu
PY - 2006
Y1 - 2006
N2 - Objective: To characterise a natural antisense transcript of connective tissue growth factor. Materials and Methods: RNA from several cell lines was analysed by RNase protection assay to detect antisense transcripts. To characterise the regulatory aspect of the transcribed area, chimeras were constructed in which the firefly luciferase gene was fused with the corresponding segment of ctgf/ccn2, and the gene expression was monitored. Results: A natural antisense transcript complementary to the 3′-untranslated region of ctgf/ccn2 mRNA in cultured human tumour cells was detected. The luciferase gene fused with the full-length antisense 3′-untranslated region showed strikingly low levels of luciferase expression compared with the control. Based on a series of deletion analyses, the major repressive element was located in the middle portion within the 3′-untranslated region, which corresponded to the antisense transcribed area. Conclusion: These results suggest that controlled expression of antisense RNAs against the 3′-untranslated region of ctgf/ccn2 mRNA may be involved in the determination of phenotypes in certain oral malignancies.
AB - Objective: To characterise a natural antisense transcript of connective tissue growth factor. Materials and Methods: RNA from several cell lines was analysed by RNase protection assay to detect antisense transcripts. To characterise the regulatory aspect of the transcribed area, chimeras were constructed in which the firefly luciferase gene was fused with the corresponding segment of ctgf/ccn2, and the gene expression was monitored. Results: A natural antisense transcript complementary to the 3′-untranslated region of ctgf/ccn2 mRNA in cultured human tumour cells was detected. The luciferase gene fused with the full-length antisense 3′-untranslated region showed strikingly low levels of luciferase expression compared with the control. Based on a series of deletion analyses, the major repressive element was located in the middle portion within the 3′-untranslated region, which corresponded to the antisense transcribed area. Conclusion: These results suggest that controlled expression of antisense RNAs against the 3′-untranslated region of ctgf/ccn2 mRNA may be involved in the determination of phenotypes in certain oral malignancies.
KW - 3′-Untranslated regions
KW - Antisense
KW - Connective tissue growth factor
KW - RNA
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UR - http://www.scopus.com/inward/citedby.url?scp=33846151439&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33846151439
VL - 18
SP - 172
EP - 179
JO - Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
JF - Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology
SN - 2212-5558
IS - 3
ER -