Expression and regulation of an antisense RNA transcript of the human connective tissue growth factor gene in human tumour cells

Seiji Kondo, Satoshi Kubota, Harumi Kawaki, Norifumi Moritani, Toshimasa Kagawa, Takaaki Ueno, Toshio Sugahara, Masaharu Takigawa

Research output: Contribution to journalArticle

Abstract

Objective: To characterise a natural antisense transcript of connective tissue growth factor. Materials and Methods: RNA from several cell lines was analysed by RNase protection assay to detect antisense transcripts. To characterise the regulatory aspect of the transcribed area, chimeras were constructed in which the firefly luciferase gene was fused with the corresponding segment of ctgf/ccn2, and the gene expression was monitored. Results: A natural antisense transcript complementary to the 3′-untranslated region of ctgf/ccn2 mRNA in cultured human tumour cells was detected. The luciferase gene fused with the full-length antisense 3′-untranslated region showed strikingly low levels of luciferase expression compared with the control. Based on a series of deletion analyses, the major repressive element was located in the middle portion within the 3′-untranslated region, which corresponded to the antisense transcribed area. Conclusion: These results suggest that controlled expression of antisense RNAs against the 3′-untranslated region of ctgf/ccn2 mRNA may be involved in the determination of phenotypes in certain oral malignancies.

Original languageEnglish
Pages (from-to)172-179
Number of pages8
JournalAsian Journal of Oral and Maxillofacial Surgery
Volume18
Issue number3
Publication statusPublished - 2006

Fingerprint

Antisense RNA
3' Untranslated Regions
Luciferases
Genes
Neoplasms
Cultured Tumor Cells
Connective Tissue Growth Factor
Firefly Luciferases
Messenger RNA
Ribonucleases
RNA
Phenotype
Gene Expression
Cell Line
human CTGF protein

Keywords

  • 3′-Untranslated regions
  • Antisense
  • Connective tissue growth factor
  • RNA

ASJC Scopus subject areas

  • Surgery

Cite this

Expression and regulation of an antisense RNA transcript of the human connective tissue growth factor gene in human tumour cells. / Kondo, Seiji; Kubota, Satoshi; Kawaki, Harumi; Moritani, Norifumi; Kagawa, Toshimasa; Ueno, Takaaki; Sugahara, Toshio; Takigawa, Masaharu.

In: Asian Journal of Oral and Maxillofacial Surgery, Vol. 18, No. 3, 2006, p. 172-179.

Research output: Contribution to journalArticle

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AU - Kondo, Seiji

AU - Kubota, Satoshi

AU - Kawaki, Harumi

AU - Moritani, Norifumi

AU - Kagawa, Toshimasa

AU - Ueno, Takaaki

AU - Sugahara, Toshio

AU - Takigawa, Masaharu

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AB - Objective: To characterise a natural antisense transcript of connective tissue growth factor. Materials and Methods: RNA from several cell lines was analysed by RNase protection assay to detect antisense transcripts. To characterise the regulatory aspect of the transcribed area, chimeras were constructed in which the firefly luciferase gene was fused with the corresponding segment of ctgf/ccn2, and the gene expression was monitored. Results: A natural antisense transcript complementary to the 3′-untranslated region of ctgf/ccn2 mRNA in cultured human tumour cells was detected. The luciferase gene fused with the full-length antisense 3′-untranslated region showed strikingly low levels of luciferase expression compared with the control. Based on a series of deletion analyses, the major repressive element was located in the middle portion within the 3′-untranslated region, which corresponded to the antisense transcribed area. Conclusion: These results suggest that controlled expression of antisense RNAs against the 3′-untranslated region of ctgf/ccn2 mRNA may be involved in the determination of phenotypes in certain oral malignancies.

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