Expression and Potential Role of Peroxisome Proliferator-Activated Receptor γ in the Placenta of Diabetic Pregnancy

N. Suwaki, Hisashi Masuyama, A. Masumoto, N. Takamoto, Y. Hiramatsu

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Peroxisome proliferator-activated receptor γ (PPARγ) is expressed predominantly in adipose tissue and is known to be involved in adipocyte differentiation and insulin sensitivity. Recent reports indicated that PPARγ-deficient mice were embryonic lethal due to abnormal placental development, suggesting that PPARγ plays an important role in normal development of placenta. On the other hand, expression of vascular endothelial growth factor (VEGF), the other important factor in placental development, has been demonstrated to be regulated by PPARγ in vascular smooth muscle cells. Also, diabetic pregnancy is often associated with defective placental functions. In order to investigate physiological roles of PPARγ and VEGF in placental development during diabetic pregnancy, we examined the expressions of PPARγ and VEGF in placentas, which were obtained from normal and streptozotocin-induced diabetic pregnant mouse, and studied in vitro effects of hyperglycemic condition and PPARγ ligands (rosiglitazone and 15-deoxy-delta(12,14)prostaglandin J2) on trophoblasts using human choriocarcinoma cell lines. In diabetic mouse placentas (n = 5), expressions of PPARγ and VEGF proteins significantly increased as compared with these in normal placenta (n = 3 or 4). In vitro studies indicated that hyperglycemic condition (42 mM) significantly enhanced the PPARγ expression and hCG production, and significantly suppressed cell proliferation, however these effects were attenuated by PPARγ ligands that accompanied with increased VEGF production. These data suggest that the PPARγ pathway might be involved in the impairment of placental development induced by high glucose conditions, and that VEGF might play some roles in this pathway.

Original languageEnglish
Pages (from-to)315-323
Number of pages9
JournalPlacenta
Volume28
Issue number4
DOIs
Publication statusPublished - Apr 2007

Fingerprint

Pregnancy in Diabetics
Peroxisome Proliferator-Activated Receptors
Placenta
Vascular Endothelial Growth Factor A
Placentation
rosiglitazone
Ligands
Pregnancy
Choriocarcinoma
Trophoblasts
Streptozocin
Vascular Smooth Muscle
Adipocytes
Smooth Muscle Myocytes
Insulin Resistance
Adipose Tissue

Keywords

  • Diabetes
  • Peroxisome proliferator-activated receptor γ
  • Placenta
  • Pregnancy
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Expression and Potential Role of Peroxisome Proliferator-Activated Receptor γ in the Placenta of Diabetic Pregnancy. / Suwaki, N.; Masuyama, Hisashi; Masumoto, A.; Takamoto, N.; Hiramatsu, Y.

In: Placenta, Vol. 28, No. 4, 04.2007, p. 315-323.

Research output: Contribution to journalArticle

Suwaki, N. ; Masuyama, Hisashi ; Masumoto, A. ; Takamoto, N. ; Hiramatsu, Y. / Expression and Potential Role of Peroxisome Proliferator-Activated Receptor γ in the Placenta of Diabetic Pregnancy. In: Placenta. 2007 ; Vol. 28, No. 4. pp. 315-323.
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