Expression and localization of messenger RNA and protein for monocyte chemoattractant protein-1 in human malignant glioma

H. Takeshima, J. I. Kuratsu, M. Takeya, Teizo Yoshimura, Y. Ushio

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Expression of monocyte chemoattractant protein-1 (MCP-1) in human glioma cell lines and surgical specimens was studied by Northern blot analysis, reverse-transcription polymerase chain reaction, in situ hybridization, and immunohistochemistry. The samples tested consisted of 11 human glioma cell lines and eight specimens of human malignant glioma (seven from glioblastomas and one from a malignant ependymoma). Messenger ribonucleic acid (mRNA) of MCP-1 was detected by either Northern blot or reverse-transcription polymerase chain reaction analysis in all cell lines and tumor specimens examined. In vivo expression of MCP-1 mRNA and protein was found predominantly in glioma cells with large and pleomorphic nuclei rather than in areas of small nucleated glioma cells. Adjacent brain tissue did not produce a significant level of MCP-1 mRNA or protein. Tumor vessels with endothelial proliferation expressed a moderate level of MCP-1 protein. Macrophages were found among the glioma cells, and the degree of macrophage infiltration was grossly correlated with the level of MCP-1 expression. The study results suggest that MCP-1 produced by the glioma cells may mediate macrophage infiltration into the glioma tissue.

Original languageEnglish
Pages (from-to)1056-1062
Number of pages7
JournalJournal of Neurosurgery
Volume80
Issue number6
Publication statusPublished - 1994
Externally publishedYes

Fingerprint

Glioma
Chemokine CCL2
Messenger RNA
Proteins
Macrophages
RNA
Northern Blotting
Reverse Transcription
Cell Line
Ependymoma
Polymerase Chain Reaction
human CCL2 protein
Glioblastoma
Tumor Cell Line
In Situ Hybridization
Immunohistochemistry
Brain
Neoplasms

Keywords

  • cytokine
  • gene expression
  • glioma
  • macrophage infiltration
  • monocyte chemoattractant protein-1

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Expression and localization of messenger RNA and protein for monocyte chemoattractant protein-1 in human malignant glioma. / Takeshima, H.; Kuratsu, J. I.; Takeya, M.; Yoshimura, Teizo; Ushio, Y.

In: Journal of Neurosurgery, Vol. 80, No. 6, 1994, p. 1056-1062.

Research output: Contribution to journalArticle

@article{24f3d9529fd2477d92e070d20ef5d2c6,
title = "Expression and localization of messenger RNA and protein for monocyte chemoattractant protein-1 in human malignant glioma",
abstract = "Expression of monocyte chemoattractant protein-1 (MCP-1) in human glioma cell lines and surgical specimens was studied by Northern blot analysis, reverse-transcription polymerase chain reaction, in situ hybridization, and immunohistochemistry. The samples tested consisted of 11 human glioma cell lines and eight specimens of human malignant glioma (seven from glioblastomas and one from a malignant ependymoma). Messenger ribonucleic acid (mRNA) of MCP-1 was detected by either Northern blot or reverse-transcription polymerase chain reaction analysis in all cell lines and tumor specimens examined. In vivo expression of MCP-1 mRNA and protein was found predominantly in glioma cells with large and pleomorphic nuclei rather than in areas of small nucleated glioma cells. Adjacent brain tissue did not produce a significant level of MCP-1 mRNA or protein. Tumor vessels with endothelial proliferation expressed a moderate level of MCP-1 protein. Macrophages were found among the glioma cells, and the degree of macrophage infiltration was grossly correlated with the level of MCP-1 expression. The study results suggest that MCP-1 produced by the glioma cells may mediate macrophage infiltration into the glioma tissue.",
keywords = "cytokine, gene expression, glioma, macrophage infiltration, monocyte chemoattractant protein-1",
author = "H. Takeshima and Kuratsu, {J. I.} and M. Takeya and Teizo Yoshimura and Y. Ushio",
year = "1994",
language = "English",
volume = "80",
pages = "1056--1062",
journal = "Journal of Neurosurgery",
issn = "0022-3085",
publisher = "American Association of Neurological Surgeons",
number = "6",

}

TY - JOUR

T1 - Expression and localization of messenger RNA and protein for monocyte chemoattractant protein-1 in human malignant glioma

AU - Takeshima, H.

AU - Kuratsu, J. I.

AU - Takeya, M.

AU - Yoshimura, Teizo

AU - Ushio, Y.

PY - 1994

Y1 - 1994

N2 - Expression of monocyte chemoattractant protein-1 (MCP-1) in human glioma cell lines and surgical specimens was studied by Northern blot analysis, reverse-transcription polymerase chain reaction, in situ hybridization, and immunohistochemistry. The samples tested consisted of 11 human glioma cell lines and eight specimens of human malignant glioma (seven from glioblastomas and one from a malignant ependymoma). Messenger ribonucleic acid (mRNA) of MCP-1 was detected by either Northern blot or reverse-transcription polymerase chain reaction analysis in all cell lines and tumor specimens examined. In vivo expression of MCP-1 mRNA and protein was found predominantly in glioma cells with large and pleomorphic nuclei rather than in areas of small nucleated glioma cells. Adjacent brain tissue did not produce a significant level of MCP-1 mRNA or protein. Tumor vessels with endothelial proliferation expressed a moderate level of MCP-1 protein. Macrophages were found among the glioma cells, and the degree of macrophage infiltration was grossly correlated with the level of MCP-1 expression. The study results suggest that MCP-1 produced by the glioma cells may mediate macrophage infiltration into the glioma tissue.

AB - Expression of monocyte chemoattractant protein-1 (MCP-1) in human glioma cell lines and surgical specimens was studied by Northern blot analysis, reverse-transcription polymerase chain reaction, in situ hybridization, and immunohistochemistry. The samples tested consisted of 11 human glioma cell lines and eight specimens of human malignant glioma (seven from glioblastomas and one from a malignant ependymoma). Messenger ribonucleic acid (mRNA) of MCP-1 was detected by either Northern blot or reverse-transcription polymerase chain reaction analysis in all cell lines and tumor specimens examined. In vivo expression of MCP-1 mRNA and protein was found predominantly in glioma cells with large and pleomorphic nuclei rather than in areas of small nucleated glioma cells. Adjacent brain tissue did not produce a significant level of MCP-1 mRNA or protein. Tumor vessels with endothelial proliferation expressed a moderate level of MCP-1 protein. Macrophages were found among the glioma cells, and the degree of macrophage infiltration was grossly correlated with the level of MCP-1 expression. The study results suggest that MCP-1 produced by the glioma cells may mediate macrophage infiltration into the glioma tissue.

KW - cytokine

KW - gene expression

KW - glioma

KW - macrophage infiltration

KW - monocyte chemoattractant protein-1

UR - http://www.scopus.com/inward/record.url?scp=0028227974&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028227974&partnerID=8YFLogxK

M3 - Article

C2 - 8189261

AN - SCOPUS:0028227974

VL - 80

SP - 1056

EP - 1062

JO - Journal of Neurosurgery

JF - Journal of Neurosurgery

SN - 0022-3085

IS - 6

ER -