Expression and localization of cFLIP, an anti-apoptotic factor, in the bovine corpus luteum

Takuo Hojo, Mohamad Omar Al-Zi'Abi, Junichi Komiyama, Noboru Manabe, Tomas J. Acosta, Kiyoshi Okuda

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11 Citations (Scopus)

Abstract

The objective of the present study was to investigate the potential mechanisms regulating cellular FLICE-like inhibitory protein (cFLIP), an anti-apoptotic factor, in the bovine corpus luteum (CL). Expression of cFLIP mRNA was highest at the developing stage and then decreased significantly during the mid, late and regressed stages (P<0.05). Western blot analysis revealed that expression of the long isoform of cFLIP (cFLIPL) protein was high during the early and developing luteal stages, remained steady during the mid and late luteal stages and then decreased significantly (P<0.05) by the regressed stage. However, the expression levels of the short isoform of cFLIP (cFLIPS) remained low during the early, developing and mid luteal stages. Immunostaining of cFLIP was strongest in the cytoplasm of luteal and non-luteal cells, including endothelial and immune cells, remained high during the early, developing and mid luteal stages and then decreased significantly (P<0.05) in the late and regressed luteal stages. Immunostaining of cFLIP was observed only in macrophage-like cells in the regressing CL. However, cultured mid luteal cells had a higher percentage of cFLIP-positive cells and a lower percentage of TUNEL-positive cells than luteal cells treated with tumor necrosis factor α (TNF)/interferon γ (IFNG; P<0.01). These results indicate downregulation of cFLIP during structural luteal regression, suggesting that cFLIP plays a survival role in the bovine CL.

Original languageEnglish
Pages (from-to)230-235
Number of pages6
JournalJournal of Reproduction and Development
Volume56
Issue number2
DOIs
Publication statusPublished - Apr 1 2010

Keywords

  • Anti-apoptotic factor
  • Apoptosis
  • Bovine
  • Cellular FLICE-like inhibitory protein (cFLIP)
  • Corpus luteum

ASJC Scopus subject areas

  • Animal Science and Zoology

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    Hojo, T., Omar Al-Zi'Abi, M., Komiyama, J., Manabe, N., Acosta, T. J., & Okuda, K. (2010). Expression and localization of cFLIP, an anti-apoptotic factor, in the bovine corpus luteum. Journal of Reproduction and Development, 56(2), 230-235. https://doi.org/10.1262/jrd.09-185S