Expression and localization of 25-Dx, a membrane-associated putative progesterone-binding protein, in the developing Purkinje cell

Hirotaka Sakamoto, K. Ukena, H. Takemori, M. Okamoto, M. Kawata, K. Tsutsui

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Neurosteroids are synthesized de novo in the brain and the cerebellar Purkinje cell is a major site for neurosteroid formation. We have demonstrated that the rat Purkinje cell actively produces progesterone de novo from cholesterol only during neonatal life and progesterone promotes dendritic growth, spinogenesis and synaptogenesis via its nuclear receptor in this neuron. On the other hand, 25-Dx, a putative membrane progesterone receptor, has been identified in the rat liver. In this study, we therefore investigated the expression and localization of 25-Dx in the Purkinje cell to understand the mode of progesterone actions in this neuron. Reverse transcription-PCR and Western immunoblot analyses revealed the expressions of 25-Dx mRNA and 25-Dx-like protein in the rat cerebellum, which increased during neonatal life. By immunocytochemistry, the expression of 25-Dx-like protein was localized in the Purkinje cell and external granule cell layer. At the ultrastructural level, we further found that 25-Dx-like immunoreactivity was associated with membrane structures of the endoplasmic reticulum and Golgi apparatus in the Purkinje cell. These results indicate that the Purkinje cell expresses the putative membrane progesterone receptor, 25-Dx during neonatal life. Progesterone may promote dendritic growth, spinogenesis and synaptogenesis via 25-Dx as well as its nuclear receptor in the Purkinje cell in the neonate.

Original languageEnglish
Pages (from-to)325-334
Number of pages10
JournalNeuroscience
Volume126
Issue number2
DOIs
Publication statusPublished - 2004
Externally publishedYes

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Progesterone-Binding Globulin
Purkinje Cells
Membranes
Progesterone
Progesterone Receptors
Cytoplasmic and Nuclear Receptors
Neurotransmitter Agents
Neurons
Golgi Apparatus
Growth
Endoplasmic Reticulum
Cerebellum
Reverse Transcription
Proteins
Western Blotting
Immunohistochemistry
Cholesterol
Polymerase Chain Reaction
Messenger RNA

Keywords

  • ABC
  • avidin-biotin-peroxidase complex
  • cerebellum
  • DAB
  • development
  • diaminobenzidine
  • inner zone-specific antigen
  • IZAg
  • neurosteroids
  • OD
  • optical density
  • PB
  • PBS
  • PFA
  • phosphate buffer
  • phosphate-buffered saline
  • progesterone
  • progesterone receptor

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression and localization of 25-Dx, a membrane-associated putative progesterone-binding protein, in the developing Purkinje cell. / Sakamoto, Hirotaka; Ukena, K.; Takemori, H.; Okamoto, M.; Kawata, M.; Tsutsui, K.

In: Neuroscience, Vol. 126, No. 2, 2004, p. 325-334.

Research output: Contribution to journalArticle

Sakamoto, Hirotaka ; Ukena, K. ; Takemori, H. ; Okamoto, M. ; Kawata, M. ; Tsutsui, K. / Expression and localization of 25-Dx, a membrane-associated putative progesterone-binding protein, in the developing Purkinje cell. In: Neuroscience. 2004 ; Vol. 126, No. 2. pp. 325-334.
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