Abstract
FKHRL1 (FOXO3a), a member of the Forkhead family of genes, has been considered to be involved in the development of breast tumors; however, the in vivo expression and activation status of FKHRL1 in breast tumors still remains unclear. We immunohistochemically demonstrated the expression and intracellular localization of FKHRL1 in human breast tumors by the novel anti-FKHRL1 antibody which is available for formalin-fixed paraffin-embedded specimens. In a total of 51 cases of benign tumors, FKHRL1 was diffusely expressed in all cases, and its intracellular localization was revealed to be cytoplasmic (inactive form) in 94% of cases of intraductal papillomas (16/17) and 91% cases of fibroadenomas (31/34), with a similar pattern to normal glandular epithelium. In invasive ductal carcinomas, 83% of the cases (93/112) diffusely expressed FKHRL1; however, unlike benign tumors, 71% of the cases (66/93) showed the nuclear-targeted, active form of FKHRL1. Moreover, activated FKHRL1 was predominantly observed in scirrhous (29/36, 81% of the cases) and papillotubular (30/38, 79% of the cases) subtypes, compared to the solid-tubular subtype (7/19, 37% of the cases). Furthermore, the cases with nuclear-targeted FKHRL1 showed a tendency to have lymph nodal metastasis with statistical significance (P <0.0001). Thus, the activation of FKHRL1 seems to be recognized as one of the specific features of invasive ductal carcinoma of the breast.
| Original language | English |
|---|---|
| Pages (from-to) | 197-205 |
| Number of pages | 9 |
| Journal | Acta Medica Okayama |
| Volume | 58 |
| Issue number | 4 |
| Publication status | Published - Aug 2004 |
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Keywords
- Breast tumors
- FKHRL1
- Intracellular localization
ASJC Scopus subject areas
- Medicine(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Expression and intracellular localization of FKHRL1 in mammary gland neoplasms. / Jin, Gui Shan; Kondo, Eisaku; Miyake, Takayoshi; Shibata, Masao; Takashima, Takako; Liu, Yi Xuan; Hayashi, Kazuhiko; Akagi, Tadaatsu; Yoshino, Tadashi.
In: Acta Medica Okayama, Vol. 58, No. 4, 08.2004, p. 197-205.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Expression and intracellular localization of FKHRL1 in mammary gland neoplasms
AU - Jin, Gui Shan
AU - Kondo, Eisaku
AU - Miyake, Takayoshi
AU - Shibata, Masao
AU - Takashima, Takako
AU - Liu, Yi Xuan
AU - Hayashi, Kazuhiko
AU - Akagi, Tadaatsu
AU - Yoshino, Tadashi
PY - 2004/8
Y1 - 2004/8
N2 - FKHRL1 (FOXO3a), a member of the Forkhead family of genes, has been considered to be involved in the development of breast tumors; however, the in vivo expression and activation status of FKHRL1 in breast tumors still remains unclear. We immunohistochemically demonstrated the expression and intracellular localization of FKHRL1 in human breast tumors by the novel anti-FKHRL1 antibody which is available for formalin-fixed paraffin-embedded specimens. In a total of 51 cases of benign tumors, FKHRL1 was diffusely expressed in all cases, and its intracellular localization was revealed to be cytoplasmic (inactive form) in 94% of cases of intraductal papillomas (16/17) and 91% cases of fibroadenomas (31/34), with a similar pattern to normal glandular epithelium. In invasive ductal carcinomas, 83% of the cases (93/112) diffusely expressed FKHRL1; however, unlike benign tumors, 71% of the cases (66/93) showed the nuclear-targeted, active form of FKHRL1. Moreover, activated FKHRL1 was predominantly observed in scirrhous (29/36, 81% of the cases) and papillotubular (30/38, 79% of the cases) subtypes, compared to the solid-tubular subtype (7/19, 37% of the cases). Furthermore, the cases with nuclear-targeted FKHRL1 showed a tendency to have lymph nodal metastasis with statistical significance (P <0.0001). Thus, the activation of FKHRL1 seems to be recognized as one of the specific features of invasive ductal carcinoma of the breast.
AB - FKHRL1 (FOXO3a), a member of the Forkhead family of genes, has been considered to be involved in the development of breast tumors; however, the in vivo expression and activation status of FKHRL1 in breast tumors still remains unclear. We immunohistochemically demonstrated the expression and intracellular localization of FKHRL1 in human breast tumors by the novel anti-FKHRL1 antibody which is available for formalin-fixed paraffin-embedded specimens. In a total of 51 cases of benign tumors, FKHRL1 was diffusely expressed in all cases, and its intracellular localization was revealed to be cytoplasmic (inactive form) in 94% of cases of intraductal papillomas (16/17) and 91% cases of fibroadenomas (31/34), with a similar pattern to normal glandular epithelium. In invasive ductal carcinomas, 83% of the cases (93/112) diffusely expressed FKHRL1; however, unlike benign tumors, 71% of the cases (66/93) showed the nuclear-targeted, active form of FKHRL1. Moreover, activated FKHRL1 was predominantly observed in scirrhous (29/36, 81% of the cases) and papillotubular (30/38, 79% of the cases) subtypes, compared to the solid-tubular subtype (7/19, 37% of the cases). Furthermore, the cases with nuclear-targeted FKHRL1 showed a tendency to have lymph nodal metastasis with statistical significance (P <0.0001). Thus, the activation of FKHRL1 seems to be recognized as one of the specific features of invasive ductal carcinoma of the breast.
KW - Breast tumors
KW - FKHRL1
KW - Intracellular localization
UR - http://www.scopus.com/inward/record.url?scp=4644354580&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4644354580&partnerID=8YFLogxK
M3 - Article
C2 - 15551757
AN - SCOPUS:4644354580
VL - 58
SP - 197
EP - 205
JO - Acta Medica Okayama
JF - Acta Medica Okayama
SN - 0386-300X
IS - 4
ER -