Expression and intracellular distribution of the G protein-coupled receptor 30 in rat hippocampal formation

KenIchi Matsuda, Hirotaka Sakamoto, Hiroko Mori, Koji Hosokawa, Akeo Kawamura, Minoru Itose, Mayumi Nishi, Eric R. Prossnitz, Mitsuhiro Kawata

Research output: Contribution to journalArticle

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Abstract

Although the expression and distribution of nuclear estrogen receptors in the hippocampus has been described, it has been proposed that the nuclear receptors may not explain all aspects of estrogen function in the hippocampus. Recently, a G protein-coupled receptor for estrogen, GPR30, was identified as a membrane-localized estrogen receptor in several cancer cell lines. In this study, we examined the expression and intracellular distribution of GPR30 in the rat hippocampal formation. We found expression of GPR30 in pyramidal cells of CA1-3 and granule cells of the dentate gyrus at both mRNA and protein levels. Specific markers for intracellular organelles and immunoelectron microscopy revealed that GPR30 was mainly localized to the Golgi apparatus and partially in the endoplasmic reticulum of the neuron but could not detect the protein at the cell surface. Expression levels were not different among male, female in proestrus and female in estrus at the adult stage, but were higher in newborn male than newborn female.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalNeuroscience Letters
Volume441
Issue number1
DOIs
Publication statusPublished - Aug 15 2008
Externally publishedYes

Fingerprint

Hippocampus
Estrogen Receptors
Estrogens
Proestrus
Immunoelectron Microscopy
Pyramidal Cells
Dentate Gyrus
Estrus
Golgi Apparatus
Cytoplasmic and Nuclear Receptors
G-Protein-Coupled Receptors
Endoplasmic Reticulum
Organelles
Membrane Proteins
Neurons
Cell Line
Messenger RNA
Membranes
rat GPR30 protein
Neoplasms

Keywords

  • Estrogen
  • GPCR
  • Hippocampus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression and intracellular distribution of the G protein-coupled receptor 30 in rat hippocampal formation. / Matsuda, KenIchi; Sakamoto, Hirotaka; Mori, Hiroko; Hosokawa, Koji; Kawamura, Akeo; Itose, Minoru; Nishi, Mayumi; Prossnitz, Eric R.; Kawata, Mitsuhiro.

In: Neuroscience Letters, Vol. 441, No. 1, 15.08.2008, p. 94-99.

Research output: Contribution to journalArticle

Matsuda, K, Sakamoto, H, Mori, H, Hosokawa, K, Kawamura, A, Itose, M, Nishi, M, Prossnitz, ER & Kawata, M 2008, 'Expression and intracellular distribution of the G protein-coupled receptor 30 in rat hippocampal formation', Neuroscience Letters, vol. 441, no. 1, pp. 94-99. https://doi.org/10.1016/j.neulet.2008.05.108
Matsuda, KenIchi ; Sakamoto, Hirotaka ; Mori, Hiroko ; Hosokawa, Koji ; Kawamura, Akeo ; Itose, Minoru ; Nishi, Mayumi ; Prossnitz, Eric R. ; Kawata, Mitsuhiro. / Expression and intracellular distribution of the G protein-coupled receptor 30 in rat hippocampal formation. In: Neuroscience Letters. 2008 ; Vol. 441, No. 1. pp. 94-99.
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