Expression and Interaction Analysis of Arabidopsis Skp1-Related Genes

Naoki Takahashi, Hirofumi Kuroda, Takashi Kuromori, Takashi Hirayama, Motoaki Seki, Kazuo Shinozaki, Hiroaki Shimada, Minami Matsui

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Specific protein degradation has been observed in several aspects of development and differentiation in many organisms. One example of such proteolysis is regulated by protein polyubiquitination that is promoted by the SCF complex consisting of Skp1, cullin, and an F-box protein. We examined the activities of the Arabidopsis Skp1-related proteins (ASKs). Among 19 annotated ASK genes, we isolated 16 of the corresponding cDNAs (ASK1, 2, 3, 4, 7, 8, 9, 10, 11, 12, 13, 14, 16, 17, 18, 19), and examined their gene products for interactions with 24 representatives of F-box proteins carrying various classes of the C-terminal domains using the yeast two-hybrid system. As a result, we found diverse binding specificities: ASK1, ASK2, ASK11 and ASK12 interacted well with COI1, FKF1, UFO-like protein, LRR-containing F-box proteins, and other F-box proteins with unknown C-terminal motifs. We also observed specific interaction between F-box proteins and ASK3, ASK9, ASK13, ASK14, ASK16 and ASK18. In contrast, we detected no interaction between any of the 12 ASK proteins and F-box proteins containing CRFA, CRFB or CRFC domains. Both histochemical and RT-PCR analysis of eight ASK genes expression revealed unique expression patterns for the respective genes.

Original languageEnglish
Pages (from-to)83-91
Number of pages9
JournalPlant and Cell Physiology
Volume45
Issue number1
DOIs
Publication statusPublished - Jan 2004
Externally publishedYes

Fingerprint

F-Box Proteins
S-Phase Kinase-Associated Proteins
F-box proteins
Arabidopsis Proteins
Arabidopsis
Genes
genes
proteins
Proteolysis
SKP Cullin F-Box Protein Ligases
Two-Hybrid System Techniques
Proteins
two hybrid system techniques
Complementary DNA
protein degradation
proteolysis
Gene Expression
Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction
yeasts

Keywords

  • Arabidopsis thaliana
  • ASK
  • F-box protein
  • GUS
  • Protein degradation
  • Yeast two-hybrid system

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Takahashi, N., Kuroda, H., Kuromori, T., Hirayama, T., Seki, M., Shinozaki, K., ... Matsui, M. (2004). Expression and Interaction Analysis of Arabidopsis Skp1-Related Genes. Plant and Cell Physiology, 45(1), 83-91. https://doi.org/10.1093/pcp/pch009

Expression and Interaction Analysis of Arabidopsis Skp1-Related Genes. / Takahashi, Naoki; Kuroda, Hirofumi; Kuromori, Takashi; Hirayama, Takashi; Seki, Motoaki; Shinozaki, Kazuo; Shimada, Hiroaki; Matsui, Minami.

In: Plant and Cell Physiology, Vol. 45, No. 1, 01.2004, p. 83-91.

Research output: Contribution to journalArticle

Takahashi, N, Kuroda, H, Kuromori, T, Hirayama, T, Seki, M, Shinozaki, K, Shimada, H & Matsui, M 2004, 'Expression and Interaction Analysis of Arabidopsis Skp1-Related Genes', Plant and Cell Physiology, vol. 45, no. 1, pp. 83-91. https://doi.org/10.1093/pcp/pch009
Takahashi, Naoki ; Kuroda, Hirofumi ; Kuromori, Takashi ; Hirayama, Takashi ; Seki, Motoaki ; Shinozaki, Kazuo ; Shimada, Hiroaki ; Matsui, Minami. / Expression and Interaction Analysis of Arabidopsis Skp1-Related Genes. In: Plant and Cell Physiology. 2004 ; Vol. 45, No. 1. pp. 83-91.
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