Expression and correlation analysis of β-adrenoceptors, VEGF, MMP-9 and caspase-3 in different phases of infantile hemangioma

Weili Yuan, Jingjian Wei, Keisuke Nakano, Mihoko Tomida, Toshiyuki Kawakami, Zhengyu Liu, Lei Xue, Xukai Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: To investigate the expression and correlation analysis of β-adrenoceptors (AR), VEGF, MMP-9 and caspase-3 in the different phases of infantile hemangioma (IH) and to find out the possible mechanism of β-AR antagonist propranolol treatment for IH. Methods: 52 fresh operative IH specimens were collected and were divided into 2 groups: 32 patients of proliferating phase and 20 patients of involuting phase. Ten specimens of normal skin were also collected as the control group. The expression of β-AR, VEGF, MMP-9 and caspase-3 were detected by immunohistochemistry. The integral optical density and positive area rate of β-AR, VEGF, MMP-9 and caspase-3 were examined using a microscopic image analysis system. The correlations among the above markers were also analyzed using Pearson correlation coefficients. Results: β1-AR and β2-AR were both strongly expressed in the proliferating and involuting hemangioma tissue. There was a significant difference (P

Original languageEnglish
Pages (from-to)51-58
Number of pages8
JournalJournal of Hard Tissue Biology
Volume22
Issue number1
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Density (optical)
Hemangioma
Matrix Metalloproteinases
Caspase 3
Adrenergic Receptors
Image analysis
Vascular Endothelial Growth Factor A
Skin
Tissue
Propranolol
Immunohistochemistry
Control Groups

Keywords

  • β-adrenoceptors
  • Caspase-3
  • Infantile hemangioma
  • MMP-9
  • VEGF

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

Expression and correlation analysis of β-adrenoceptors, VEGF, MMP-9 and caspase-3 in different phases of infantile hemangioma. / Yuan, Weili; Wei, Jingjian; Nakano, Keisuke; Tomida, Mihoko; Kawakami, Toshiyuki; Liu, Zhengyu; Xue, Lei; Wang, Xukai.

In: Journal of Hard Tissue Biology, Vol. 22, No. 1, 2013, p. 51-58.

Research output: Contribution to journalArticle

Yuan, Weili ; Wei, Jingjian ; Nakano, Keisuke ; Tomida, Mihoko ; Kawakami, Toshiyuki ; Liu, Zhengyu ; Xue, Lei ; Wang, Xukai. / Expression and correlation analysis of β-adrenoceptors, VEGF, MMP-9 and caspase-3 in different phases of infantile hemangioma. In: Journal of Hard Tissue Biology. 2013 ; Vol. 22, No. 1. pp. 51-58.
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AU - Kawakami, Toshiyuki

AU - Liu, Zhengyu

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