Expression and correlation analysis of β-adrenoceptors, VEGF, MMP-9 and caspase-3 in different phases of infantile hemangioma

Weili Yuan, Jingjian Wei, Keisuke Nakano, Mihoko Tomida, Toshiyuki Kawakami, Zhengyu Liu, Lei Xue, Xukai Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: To investigate the expression and correlation analysis of β-adrenoceptors (AR), VEGF, MMP-9 and caspase-3 in the different phases of infantile hemangioma (IH) and to find out the possible mechanism of β-AR antagonist propranolol treatment for IH. Methods: 52 fresh operative IH specimens were collected and were divided into 2 groups: 32 patients of proliferating phase and 20 patients of involuting phase. Ten specimens of normal skin were also collected as the control group. The expression of β-AR, VEGF, MMP-9 and caspase-3 were detected by immunohistochemistry. The integral optical density and positive area rate of β-AR, VEGF, MMP-9 and caspase-3 were examined using a microscopic image analysis system. The correlations among the above markers were also analyzed using Pearson correlation coefficients. Results: β1-AR and β2-AR were both strongly expressed in the proliferating and involuting hemangioma tissue. There was a significant difference (P<0.01) for the expression of β1-AR and β2-AR between the hemangioma tissue and the normal skin. VEGF and MMP-9 were strongly expressed in the proliferating hemangioma and weakly expressed in the involuting hemangioma, and there was a significant difference (P<0.01) for the expression of VEGF and MMP-9 between the proliferating hemangioma and involuting hemangioma or between the proliferating hemangioma and the normal skin. There was a significantly positive correlation between the expression of β-AR and the expression of VEGF and MMP-9 in the proliferating phase of IH. There was a significant difference (P<0.01) for the expression of caspase-3 between the proliferating hemangioma and involuting hemangioma or between the involuting hemangioma and the normal skin. There was a significantly negative correlation between the expression of β-AR, VEGF and MMP-9 and the expression of caspase-3 in the proliferating phase of IH. Conclusions: β-AR, VEGF, MMP-9 and caspase-3 have an effect on the pathogenesis of hemangioma. β-AR antagonist propranolol might treat IH by the mechanism of regulating the β-AR/VEGF/MMP-9/caspase-3 pathway, inhibit the proliferation of hemangioma, promote the apoptosis of hemangioma, and eventually bring about the regression of IH.

Original languageEnglish
Pages (from-to)51-58
Number of pages8
JournalJournal of Hard Tissue Biology
Volume22
Issue number1
DOIs
Publication statusPublished - Mar 5 2013
Externally publishedYes

Keywords

  • Caspase-3
  • Infantile hemangioma
  • MMP-9
  • VEGF
  • β-adrenoceptors

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry
  • Biomaterials
  • Orthopedics and Sports Medicine
  • Dentistry(all)
  • Cell Biology

Fingerprint Dive into the research topics of 'Expression and correlation analysis of β-adrenoceptors, VEGF, MMP-9 and caspase-3 in different phases of infantile hemangioma'. Together they form a unique fingerprint.

  • Cite this