Expression and Ca2+ dependency of plasma membrane K+ channels of tobacco suspension cells adapted to salt stress

Naoki Kasukabe, Megumi Watanabe-Sugimoto, Ken Matsuoka, Eiji Okuma, Ichiro Obi, Yoshimasa Nakamura, Yasuaki Shimoishi, Yoshiyuki Murata, Tadaaki Kakutani

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The expression of plasma membrane K+ channels of NaCl-adapted tobacco suspension cells and effects of extracellular Ca2+ on plasma membrane K+ channels were investigated. Reverse transcription-PCR (RT-PCR) analysis showed that expression of TORK1, which encodes a K+ channel, was much lower in NaCl-adapted cells than in NaCl-unadapted cells. The magnitude of the outward K+ currents of NaCl-adapted as well as NaCl-unadapted cells decreased with increasing extracellular Ca2+ but there is no significant difference in Ca2+ dependency of the K + current. These analyses suggest that reduction of the number of K+ channels might cause NaCl adaptation of cells through the decrease of outward K+ currents.

Original languageEnglish
Pages (from-to)1674-1677
Number of pages4
JournalPlant and Cell Physiology
Volume47
Issue number12
DOIs
Publication statusPublished - Dec 2006

Keywords

  • Extracellular Ca
  • K channel
  • Nicotiana tabacum
  • Plasma membrane
  • Salt adaptation

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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