Expression analysis of pediatric solid tumor cell lines using oligonucleotide microarrays.

Daniel H. Wai, Karl Ludwig Schaefer, Alexander Schramm, Eberhard Korsching, Frans Van Valen, Toshihumi Ozaki, Werner Boecker, Lothar Schweigerer, Barbara Dockhorn-Dworniczak, Christopher Poremba

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

We identified patterns of differentially-expressed genes in cell lines derived from several pediatric solid tumors. Affymetrix Human Cancer G110 Arrays, carrying 1,700 cancer-associated genes, were applied to a panel of 11 cell lines originating from Ewing tumors (ETs), neuroblastomas, and malignant melanoma of soft parts. Hierarchical clustering clearly differentiated these 3 entities and revealed groups of 75, 102, and 36 gene probe-sets exhibiting tumor-type specific up-regulation in these cell lines, respectively. Whereas ET lines demonstrated increased expression of microtubule-associated protein tau (MAPT), protein phosphatase 1 regulatory subunit 1A (PPP1R1A), NIMA (never in mitosis gene a)-related kinase 2 (NEK2), and cyclin D1 (CCND1), neuroblastoma samples exhibited high expression of wingless-type mouse mammary tumor virus integration site family member 11 (WNT11), Drosophila frizzled homolog 2 (FZD2), and adenomatous polyposis coli (APC) which are involved in regulating free beta-catenin levels. These genes likely maintain tumor-specific characteristics and participate in key downstream regulatory mechanisms. We also correlated the expression levels of up-regulated genes in ETs with their chromosomal localization and compared these data to the comparative genomic hybridization profiles of the cell lines. We demonstrate that gains of genetic material contribute essentially to differential gene expression.

Original languageEnglish
Pages (from-to)441-451
Number of pages11
JournalInternational Journal of Oncology
Volume20
Issue number3
Publication statusPublished - Mar 2002
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wai, D. H., Schaefer, K. L., Schramm, A., Korsching, E., Van Valen, F., Ozaki, T., Boecker, W., Schweigerer, L., Dockhorn-Dworniczak, B., & Poremba, C. (2002). Expression analysis of pediatric solid tumor cell lines using oligonucleotide microarrays. International Journal of Oncology, 20(3), 441-451.